|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
|
Fruiting bag in vitro
#22100565 - 08/16/15 09:47 PM (8 years, 5 months ago) |
|
|
So I have this grow bag
I inoculated 6 days ago, and i can see mycelium growing. My question is, what do I need to do in order to fruit in vitro? When it's fully colonized do I mix the rye berries with the manure? Do I need to somehow dunk it, or open the bag and mist?
Here's a picture of the colonization progress in my bag:
Edited by dirttoldfart (08/19/15 09:17 AM)
|
PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
|
|
You should break it up and mix it with a brick of coco coir, 5 quarts of water and 2 quarts of vermiculite inside of a 66 quart 'monotub'.
These links should help:
http://www.shroomery.org/forums/showflat.php/Number/11916595
http://www.shroomery.org/forums/showflat.php/Number/8231041/fpart/1/vc/1
|
dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
|
|
I'm honestly just looking to fruit it in the bag, if possible. I've seen some people do it with brf jars so I was thinking that it would probably work with this as well.
|
PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
|
|
You'll get about 40x more mushrooms if you spawn it, but you can fruit it inside the bag...
Just cut slits in the top when its finished colonizing and keep it misted in there.
Total waste IMO though!
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
Quote:
PirateSwazey said: You'll get about 40x more mushrooms if you spawn it, but you can fruit it inside the bag...
Just cut slits in the top when its finished colonizing and keep it misted in there.
Total waste IMO though! 
 if pirate posted more u'd all learn a lot more. but i guess if he was here more, he'd grow a lot less 
balance, u know. he's da man. listen to his wise words when u encounter them
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
|
|
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
^^^humility is the first sign of a great gower
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
|
|
Hmm, it sounds like this is the way to go then. Would this be good to use as a substrate for a monotub? Also, would I mix in the manure that's already in the top half of my grow bag or what I not want to include it?
http://out-grow.com/mushroom-growing-substrates-c-4/5050-horse-manure-and-straw-mix-mushroom-substrate-p-68.html
Thanks a lot for your help
|
KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
|
|
Quote:
dirttoldfart said: Hmm, it sounds like this is the way to go then. Would this be good to use as a substrate for a monotub? Also, would I mix in the manure that's already in the top half of my grow bag or what I not want to include it?
http://out-grow.com/mushroom-growing-substrates-c-4/5050-horse-manure-and-straw-mix-mushroom-substrate-p-68.html
Thanks a lot for your help
There's a lot of risk to a mono tub. What you can do instead is wait for your bag to colonize more of your grains and then you break up the grains and massage and mix them really well into the other substrate in your bag. Just massage the bag until it's really thoroughly mixed all up. It will look completely uncolonized at that point, but don't worry because colonization will be really fast after you break it up and mix it all up.
Then, when it FULLY recolonizes, just cut maybe 5 or 6 2-3 inch slits in the bag to give it more air and be sure to mist once or twice lightly a day.
You'll probably get better and bigger results if you cut the top of the bag off completely to about an inch or two above the top of your substrate after it re-colonizes and put it in a SGFC (lots of teks on this) and mist it once or twice a day. After your first harvest, fill the bag with water and let it soak up overnight then pour out the water in the morning.
A monotub is no sure thing, I guarantee you. The risk of contamination goes WAY UP when you do it for the first time. Your chances of having a successful grow are much greater keeping it in the bag ... contam risk is very low. Going to a mono tub you may get nothing at all especially since you have fully sterilized horse manure in that bag which has a tendency to contaminate because it was sterilized and not pasteurized. Sterilized is fine in the bag but when you take it out, the risk goes up. I think its possible the people advising a mono tub didn't check your link to see what kind of bag you bought and think it's al just grain, which it isn't.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (08/16/15 10:53 PM)
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Fruiting bag in vitro [Re: KauaiOrca]
#22100850 - 08/16/15 10:58 PM (8 years, 5 months ago) |
|
|
Quote:
A monotub is no sure thing, I guarantee you. The risk of contamination goes WAY UP when you do it for the first time.
although i agree with ur premise, practice makes perfect. as an aside, yea, sterilized, UNCOLONIZED manure is a risk in open air. sterilized, COLONIZED manure though, is no different than colonized grain spawn.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
|
Re: Fruiting bag in vitro [Re: KauaiOrca]
#22100887 - 08/16/15 10:59 PM (8 years, 5 months ago) |
|
|
Maybe a monotub will be a future project then, I don't need to grow a huge amount of mushrooms anyway. Thanks for the advice, I'll post updates as things move along.
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
if u don't need a lot, then yea, bulk ain't the name of that game.
but bulk growing is here to stay, sorry folks
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
|
|
Quote:
blindingleaf said: if u don't need a lot, then yea, bulk ain't the name of that game.
but bulk growing is here to stay, sorry folks 
Nothing wrong with bulk growing and it's hear to stay for a lot of people that want to grow big crops of mushrooms. I think there are some people, though, especially just starting out, that want the highest possible chance of having at least some success, maybe not hundreds and hundreds of mushrooms, but a few dozen? Invitro bags have a pretty high rate of success, especially if you put them in a SGFC and I'd bet that the number of people that try for a full blown mono tub grow on their very first effort that fail for one of many reasons (contams, don't get the humidity/FAE dialed in right, etc) is fairly high and then many get nothing out of the grow, lose a couple of months in the process and have to start over.
If he was to have at least a somewhat successful grow, he could also harvest some fairly clean pins, try agar and have a self sustaining system going right from the get go. I think what really makes this hobby fun is when you get some good agar plates going and/or some clean LC and you start doing the whole process without the risk of vendor MS syringes. Learning how to do that is definitely the key to the whole thing. Whether you go from there to bulk grows or other methods like PF Tek, Monotubs, Mudabottles, V-Tek, etc. depends on how much you want to grow and how important it is to have a stealthy (or not) set up. Muda Bottles are, for me, perfect because they're practically contamination bullet proof, you can grow small amounts and test a lot of cultures simultaneously in a really small space. If I were to grow a couple of monotubs I'd end up throwing out most of the harvest as they just produce so many fruits.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Fruiting bag in vitro [Re: KauaiOrca]
#22101539 - 08/17/15 05:33 AM (8 years, 5 months ago) |
|
|
Quote:
I think what really makes this hobby fun is when you get some good agar plates going and/or some clean LC and you start doing the whole process without the risk of vendor MS syringes. Learning how to do that is definitely the key to the whole thing. Whether you go from there to bulk grows or other methods like PF Tek, Monotubs, Mudabottles, V-Tek, etc. depends on how much you want to grow and how important it is to have a stealthy (or not) set up.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
am I the only one who thinks the bag already looks fucked?
|
Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 1 day
|
|
This is the way grown people discuss things. There's a lot of gold in this thread already. Good stuff.
-------------------- "He's not altogether dense, but he's not altogether there."
|
dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
|
|
What makes you say that? To me it looks just like a lot of the other poctures of colonizing bags i could find
|
KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
|
|
Quote:
dirttoldfart said: What makes you say that? To me it looks just like a lot of the other poctures of colonizing bags i could find
Looks fine to me. Some may not realize that bag has grains on the bottom and a dung substrate on top that hasn't been mixed up yet.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
Quote:
dirttoldfart said: What makes you say that? To me it looks just like a lot of the other poctures of colonizing bags i could find
the myc looks pretty bacterial to me, maybe even a bit mold in there. its white but it aint alright.
|
dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
|
|
Now I'm worried. Where are you seeing it? I can't see anything in there besides mycelium and grains.
|
|