|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
|
Experimenting with the contaminated jars
#22099600 - 08/16/15 05:20 PM (8 years, 5 months ago) |
|
|
Nearing full colonization on my first PF tek, B+ cubensis in BRF. 2 jars have a strong mycelium, but appear to have a dark brown contamination. Instead of just tossing these in the garbage, im viewing this as an opportunity to experiment with these two cakes.
I plan to construct a mini, one-cake fc for one when it seems ready.
For the other jar, I plan on mixing it with a larger substrate as you would for a monotub.
Any thoughts on this? I probably wont eat the fruit unless it comes out looking immaculate, but this could be a valuable low risk learning experience. Also is there any reason not to collect spore prints if it works out? I was thinking i might not want more of the same genetics that have proven themselves susceptible to contam but idk.
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
Re: Experimenting with the contaminated jars [Re: SoulButter]
#22100254 - 08/16/15 08:04 PM (8 years, 5 months ago) |
|
|
Pics of the cakes in question please. Fruiting and/or spawning to bulk contaminated cakes severely ups the chance that contamination will be bred which is totally against the goal(s) of the mushroom cultivator. Any fruits generated from a contaminated substrate will be fine to eat as long as the contamination it's self ISN'T growing upon the fruitbody.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Experimenting with the contaminated jars [Re: hamloaf]
#22100280 - 08/16/15 08:12 PM (8 years, 5 months ago) |
|
|
Everytime i read one of your posts like that i feel like i'm reading a book
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
|
Re: Experimenting with the contaminated jars [Re: hamloaf]
#22100287 - 08/16/15 08:14 PM (8 years, 5 months ago) |
|
|
The only thing I could do was remove the area surrounding the contamination when mixing into the coir. Mixed one colonized brf cake into a coir/Verm "minibulk" substrate. It is now incubating. The other contam cake is still untouched.
|
Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 6 months
|
Re: Experimenting with the contaminated jars [Re: SoulButter]
#22100451 - 08/16/15 09:08 PM (8 years, 5 months ago) |
|
|
i can't say for sure but that first jar doesn't look like its cube myc. i really wouldnt mess with that but of course to each his own.
what did it smell like?
also you should not have foil underneath your lid. if you have your dry verm barrier that is the only protection you need. anything extra covering it just invites problems. except maybe micropore tape ive heard but its still unnecessary
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
Re: Experimenting with the contaminated jars [Re: Mdahmer] 1
#22100973 - 08/16/15 11:17 PM (8 years, 5 months ago) |
|
|
You are correct. Those holes in the lid not only serve as inoculation points they also serve as the pathway for your gases to exchange. The reason that covering your gas exchange holes invites problems is because when gas exchange holes are covered gasses are not able to exchange. When gasses aren't allowed to exchange anaerobic environments are created within the media vessel and substrate. Anaerobic environments thwart and/or stall mushroom mycelial growth and create an environment that favors the growth of mold and bacteria.
That being said, foil UNDER the jar lids on the PF tek? That's a new one.
|
Splinta
Stranger

Registered: 06/17/15
Posts: 94
Last seen: 8 years, 9 days
|
Re: Experimenting with the contaminated jars [Re: hamloaf]
#22101107 - 08/17/15 12:10 AM (8 years, 5 months ago) |
|
|
Ok yes foil underneath the lid of a jar is a new one and slightly funny But looking at it from a noob point of view I see where he was trying to go a little above and beyond being sterile I commend you for trying hard SoulButter But everything is done the way it is for a purpose Best info I can give is to try to follow the tek to the T step by step Sounds silly but we all mess up when we cut corners
|
SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
|
Re: Experimenting with the contaminated jars [Re: Splinta]
#22101479 - 08/17/15 04:54 AM (8 years, 5 months ago) |
|
|
This is how I read to do it. There are two layers of foil, one that I inoculated through, and one to cover up the inoculation holes. So there's no jar lid but probably no gas exchange as well. It just smelled like live mushrooms.. And yes, I have the dry verm layer on each jar, though interestingly enough, it was half colonized with the white mycelium (that I hope is cubensis??).
|
SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
|
Re: Experimenting with the contaminated jars [Re: SoulButter]
#22101708 - 08/17/15 07:14 AM (8 years, 5 months ago) |
|
|
Also my healthy myc jars are sealed up like this too, and almost fully colonized. Should I get some gas exchange going this late in? We were going to fruit in a week or two
|
Peble
The engineer

Registered: 04/04/11
Posts: 64
|
Re: Experimenting with the contaminated jars [Re: SoulButter]
#22102019 - 08/17/15 09:36 AM (8 years, 5 months ago) |
|
|
Just be careful brotha. I recently decided to just throw contams out. You don't know what's growing in there and letting it birth bacteria and mold in your house is not worth it on your lungs and can cause future project contamination. Just read more teks and have patience my friend
-------------------- The happiest person is he who thinks the most interesting thoughts.
|
SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
|
Re: Experimenting with the contaminated jars [Re: Peble]
#22102135 - 08/17/15 10:13 AM (8 years, 5 months ago) |
|
|
Quote:
Peble said: Just be careful brotha. I recently decided to just throw contams out. You don't know what's growing in there and letting it birth bacteria and mold in your house is not worth it on your lungs and can cause future project contamination. Just read more teks and have patience my friend 
luckily im moving in a week or two. The next attempt will either be a Golden Teacher monotub, OR a B+ monotub with fresh spores hopefully. Still in the research phase trying to find the best method. I have wbs, coco coir, vermiculite, perlite, and brown rice flour to work with.
|
|