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OfflineDinkinFlicka84
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Transfered agar too soon, white dot everywhere. Help?
    #22098971 - 08/16/15 02:27 PM (8 years, 5 months ago)

I used MS and inoculated my agar plates 7 days ago. It had been a while since my last agar experience, so I was a little rusty with the procedure regarding transfers or cleaning up the mycelium.

I used 2 drops of MS (from a sponsor, not homemade) from 3 different strains, so the likelihood of all 3 being bad MS is slim.

Despite only 2 drops of MS, I saw 3-7 white colonizing dots, however I don't know if they're contaminant or the right mycelium. There area also 1-2 large green colonizing spots, which are obviously contaminated, but I figured it was just a mold spore growing on my agar. Now I feel that it was the MS drop, which is why it's so much bigger than the little dots.

Any thoughts? Also, where should I go from here? I made some cloned agar as well, just to make sure I'm growing something in my plates to get started on a grow. Thanks.

I posted all of my dishes just to see if someone might pick a few out sections within a plate that a


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OfflineMdahmer
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: DinkinFlicka84]
    #22098986 - 08/16/15 02:32 PM (8 years, 5 months ago)

it looks like all of that is contam mold man.

what is your SAB procedure? like how do you set it up and all


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Invisibleblindingleaf
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: Mdahmer]
    #22099031 - 08/16/15 02:46 PM (8 years, 5 months ago)

u pulled from the right ones dude.  good choice.
just keep an eye on the transfers and the area around them.


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OfflineChakatron
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: blindingleaf]
    #22099081 - 08/16/15 03:03 PM (8 years, 5 months ago)

Trich :cannotunsee:


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OfflineDinkinFlicka84
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: blindingleaf]
    #22100543 - 08/16/15 09:35 PM (8 years, 5 months ago)

How can you tell that I pulled from the right ones? I just want to know so I can see for my self next time.

The plates with multiple white spots are the original plates that I used MS to inoculate. Oddly enough though, is that I don't see any actual MS mycelium. I put 2 drops per section down, but I only see a scatter plot of white dots that are most likely mold.

I'll keep you guys updated on what happens with pics. Thanks for the advice.


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Re: Transfered agar too soon, white dot everywhere. Help? [Re: DinkinFlicka84]
    #22100553 - 08/16/15 09:39 PM (8 years, 5 months ago)

Spores start as little white dots, that's what you transferred. You'll find out if it's for sure cube myc on those next plates you made.


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OfflineMdahmer
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: PirateSwazey]
    #22100559 - 08/16/15 09:44 PM (8 years, 5 months ago)

he used a syringe though im pretty sure. if he scraped a print it might have all those satellites but if he dropped spore solution on a plate in one or two spots then all those white dots showed up everywhere looking cottony i would think they were mold colonies unless one happened to be right where the drop was put.

but yea just watch the plates you transferred to hopefully you got something good


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Re: Transfered agar too soon, white dot everywhere. Help? [Re: Mdahmer]
    #22100564 - 08/16/15 09:46 PM (8 years, 5 months ago)

Spores from a syringe do that too


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InvisibleInocuole
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: PirateSwazey]
    #22100620 - 08/16/15 10:04 PM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Spores from a syringe do that too



:noargument:


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OfflineMdahmer
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: Inocuole]
    #22100670 - 08/16/15 10:23 PM (8 years, 5 months ago)

i have to admit to never using a spore syringe to my agar so maybe i should have stated that lol.

it just seems like if you put a drop in one spot it all the spores would be right there. unless you like tilted the plate and let it run all around i guess.


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InvisibleInocuole
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: Mdahmer]
    #22101124 - 08/17/15 12:17 AM (8 years, 5 months ago)

Unless you know to make a divot for your drop to land in, and even if you do, it still kind of micro-splashes and little bits of spores end up everywhere.


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InvisibleMad Season
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: Inocuole]
    #22101135 - 08/17/15 12:23 AM (8 years, 5 months ago)

Just put a drop on a loop and swipe it, or tilt it so it makes a spiral, but doesn't touch the walls. I like it when it grows in different areas. Much better than everything, contams included, germinating in 1 spot.


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Invisibletahoe
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: PirateSwazey]
    #22101359 - 08/17/15 02:36 AM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Spores from a syringe do that too



Especially when 2 drops are used.
One drop is usually too much liquid for agar.
What I do if I find myself going from syringe to agar is I press the plunger just enough to get a tiny ball of water forming on the end of the needle. Not big enough to fall off the needle as a drop, but just enough to be able to drag it across the agar and pull it off the needle.


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OfflineDinkinFlicka84
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: tahoe]
    #22104743 - 08/17/15 10:04 PM (8 years, 5 months ago)

That seems like a great idea. I have some inoculating loops that I've never used before, so I'm excited to see the difference.

Now, if ALL of the little fuzzy dots that have been getting significantly bigger, are only mold spores, then where are the cube spores? I'm inexperienced in agar as I'm sure is obvious, so I just read as much as possible.

The only issue now is when I get conflicting views on whether or not MS syringes can make a splash that would cash multiple inoculation points.

Now, I just opened 6 tubs where my bulk was colonizing for 14 days and 1/6 was completely triched out, and 2/6 had these mold-ish looking spots on the surface. Any idea what I'm dealing with? Please look closely because it's hard to notice at first.




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Edited by DinkinFlicka84 (08/17/15 10:07 PM)


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Invisibletahoe
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: DinkinFlicka84]
    #22105010 - 08/18/15 12:08 AM (8 years, 5 months ago)

It also doesn't seem like a good idea to have two different strains on the same petri. Seems like it would be easy to mix things up. Next thing you know you are donating what you think are burma spores to fsr, but really are b+ or chitlin


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineDinkinFlicka84
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Re: Transfered agar too soon, white dot everywhere. Help? [Re: tahoe]
    #22107461 - 08/18/15 04:29 PM (8 years, 5 months ago)

Any idea what this mold is? Or if it is even mold? I'm deciding to salt it and put it in a tub, or to leave it alone and put in my GH.




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