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Offlinestarflight
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Registered: 04/30/15
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Red filament contamination on Pan Cyan mycelium?
    #22098681 - 08/16/15 12:42 PM (8 years, 5 months ago)

I have 10 agar plates that are part of a second-round isolation attempt of some panaeolus cyanescens mycelium and most are showing this reddish filament-like stuff in the middle of the plates.

Originally had a pan cyan monotub get contaminated very badly with pin mold and I used a washed fruit body from that tub to make 10 or 15 agar plates to isolate the mycelium from the mold. Ended up with a few plates that looked good. Using the cleanest looking plate from that set, I grabbed grain of rice sized pieces of mycelium from leading edge of growth and created 10 more agar plates.

Now this second round of agar plates is starting to show some reddish right in the middle. It was hard to capture the color and the filaments in the pictures. Some of the red looks like it is mycelium with a reddish color. The first picture looks like it shows a large dark spot in the middle but that is mainly shadow from the angle of my light. From the top, the worst plates just look lightly pink in the middle. The second pic is what most of my plates look like. This one has a tiny bit of red near the bottom middle of the myc.

Lipstick mold looks closest to what I see but it's not a perfect match. I'm hoping against hope that this could be mycelium metabolites... Anyone point me in the right direction?





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InvisibleMad Season
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Registered: 09/16/12
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Re: Red filament contamination on Pan Cyan mycelium? [Re: starflight]
    #22099251 - 08/16/15 03:50 PM (8 years, 5 months ago)

Red is never a good sign. However in the pics they look healthy


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InvisiblePirateSwazey
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Re: Red filament contamination on Pan Cyan mycelium? [Re: Mad Season]
    #22100616 - 08/16/15 10:03 PM (8 years, 5 months ago)

Just transfer from the other side it looks good.


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