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OfflineShamanic Scientist
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Pics of my second grow (cubensis monotub)
    #22095690 - 08/15/15 05:14 PM (8 years, 5 months ago)

Here are a few pics from my second grow. I was able to harvest a few dried ounces from my first monotub despite not doing everything by-the-book. For my second time around, I am doing a standard monotub with spawn from rye grain jars inoculated with MS cubensis syringes. I noticed my first pins this morning, and these pics were taken this evening.

Critiques and questions are very welcome. Things will probably go pretty smoothly with this one. There's a tiny patch where I'm not seeing growth that you'll be able to see in the last pic below. Full disclosure: I put this tub into fruiting conditions a little earlier than I should have due to some time constraints I'm facing.

My goal with this grow, beyond a decent harvest, is to start cloning. My plan is to sterilize some 2-3% honey water in syringes, and clone a few of the earliest specimens. After I take tissue samples from each into separate syringes, I plan to expose the fruits to air and see which ones are the bluest as a proxy for potency, and use the syringes from those samples to start my next generation.







Edited by Shamanic Scientist (08/15/15 05:16 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Pics of my second grow (cubensis monotub) [Re: Shamanic Scientist]
    #22095726 - 08/15/15 05:22 PM (8 years, 5 months ago)

bluing doesn't correlate to potency
you should take clone tissue to agar first and use agar to inoculate any LC(sugar water)

nice tubs.


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OfflineShamanic Scientist
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Re: Pics of my second grow (cubensis monotub) [Re: bodhisatta]
    #22095739 - 08/15/15 05:27 PM (8 years, 5 months ago)

Is that just something known from experience? I thought the amount on bluing is an indicator of psilocybin content, so more blue should mean more psilocybin. Now, I also know that possible to have a potent shroom that doesn't have a lot of bluing, e.g. because the psilocybin:psilocin ratio is low, but why wouldn't a lot of bluing vs. very little bluing be a decent indicator? Is the psilocybin:psilocin ratio highly variable due to genetics?


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InvisibleMad Season
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Re: Pics of my second grow (cubensis monotub) [Re: Shamanic Scientist]
    #22095744 - 08/15/15 05:29 PM (8 years, 5 months ago)

His words from another thread:
Quote:

bodhisatta said:
mycelium bruises blue and there's plenty of studies that find almost no actives in the mycelium. variegatic acid and other non active indoles are probably at play.




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Re: Pics of my second grow (cubensis monotub) [Re: Shamanic Scientist]
    #22095753 - 08/15/15 05:32 PM (8 years, 5 months ago)



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OfflineShamanic Scientist
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Re: Pics of my second grow (cubensis monotub) [Re: bodhisatta]
    #22095765 - 08/15/15 05:35 PM (8 years, 5 months ago)

Cool, thanks for the advice. :cool:


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Re: Pics of my second grow (cubensis monotub) [Re: Shamanic Scientist]
    #22099732 - 08/16/15 05:55 PM (8 years, 5 months ago)

Second day after first pins emerged:







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Offlinetetherface
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Re: Pics of my second grow (cubensis monotub) [Re: Shamanic Scientist]
    #22100539 - 08/16/15 09:34 PM (8 years, 5 months ago)

lookin good bro definitely a beautiful 2nd grow! you should defiantly find a nice cluster and clone it although I've never heard of cloning with a syringe full of sugar based LC? i use 9er tek cloning(macerated tissue) and go to whatever pf jar GWLC or straight to grain normally i just go to a pf jar with 10cc of the 9er clone solution and about 4-5 days later i take my fully colonized cake and slurry it out to 20 quarts of oats shit works great for me i dunno what your plans are or how far along your sterile technique is but its defiantly something to think about heres the link  http://www.shroomery.org/forums/showflat.php/Number/10648149
keep up the good work :thumbup::thumbup::thumbup:


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Re: Pics of my second grow (cubensis monotub) [Re: tetherface]
    #22109989 - 08/19/15 07:15 AM (8 years, 5 months ago)

Here's what things look like this morning. My 2'' diameter holes where the polyfill is sticking through will give you a sense of scale. The veils on some of these guys look like they're starting to break. I'd be interested to here what people think about the size/morphology of these guys, and whether it says anything about how I might improve my conditions.





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