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OfflineGrim767
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first monotub a fail ?
    #22092616 - 08/14/15 08:22 PM (8 years, 5 months ago)

Hey guys after searching the forum I've come to realize I have bacterial contam in monotub due to questionable spawn. I say questionable because in the 6 jars I used there was about 1% at bottom that was... for lack of a better word mushy and some what gel like. I decided to try to salvage what I though to be the healthy myc and spawn that leaving the bottoms of jar behind. After prepping coir/verm using Damions bucket tek I spawned to tub 10 days later it's about 95% coloni zed and colonization is slowed I lifted the lid a tad to check smell and smells like sweet apples... I'll include some pics but was wondering what you guys think should I just toss it or try to fruit it and mabe get flush?



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OfflinetaGyo
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Re: first monotub a fail ? [Re: Grim767]
    #22092630 - 08/14/15 08:26 PM (8 years, 5 months ago)

Bacterial will still pin. Give it time and fruit that.

If any color of the rainbow shows up toss immediately.


Edited by taGyo (08/14/15 08:26 PM)


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OfflineGrim767
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Re: first monotub a fail ? [Re: taGyo]
    #22092638 - 08/14/15 08:29 PM (8 years, 5 months ago)

Thanks for input man I thought for sure tub was goner glad to hear I could still get something from it


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflinetaGyo
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Re: first monotub a fail ? [Re: Grim767]
    #22092642 - 08/14/15 08:30 PM (8 years, 5 months ago)

Watch it closely though. I wouldn't fruit it near other shit either.

http://www.shroomery.org/forums/showflat.php/Number/17332777


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineGrim767
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Re: first monotub a fail ? [Re: taGyo]
    #22092648 - 08/14/15 08:32 PM (8 years, 5 months ago)

Yeah already have it isolated ready to nuke the shit out of it with bleach if goes south


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I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflineMdahmer
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Re: first monotub a fail ? [Re: Grim767]
    #22092733 - 08/14/15 08:58 PM (8 years, 5 months ago)

just for future reference you can take the lid off to get a nice clear picture it won't mess anything up


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OfflineGrim767
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Re: first monotub a fail ? [Re: Mdahmer]
    #22092742 - 08/14/15 09:01 PM (8 years, 5 months ago)

Good to know thanks


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I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflineGrim767
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Re: first monotub a fail ? [Re: Grim767]
    #22126122 - 08/22/15 09:35 AM (8 years, 5 months ago)

So went ahead and put it into fruiting conditions and 24 hours later have about 12 pins so far some pretty big lol just wanted to say thanks for the info thought I'd give you guys update. :smile:


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflineGrim767
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Re: first monotub a fail ? [Re: Grim767]
    #22142437 - 08/25/15 06:46 PM (8 years, 5 months ago)


Just an update


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I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflinePsilosoulful

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Re: first monotub a fail ? [Re: Grim767]
    #22142449 - 08/25/15 06:49 PM (8 years, 5 months ago)

Nice! Looks like you coulda had a better pinset if it was cased, but not bad. My other suggestion would be to readjust and dial in the polyfill better, I see some fuzzy feet on the bases of those mushies.


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Offlinetetherface
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Re: first monotub a fail ? [Re: Grim767]
    #22142500 - 08/25/15 06:59 PM (8 years, 5 months ago)

at least your getting fruits out of it bro :thumbup: i'm honestly surprised it did that well if there was sludge in the bottom of the spawn you used. Did you ever figure out the source of the bacteria?


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OfflinePsilosoulful

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Re: first monotub a fail ? [Re: tetherface]
    #22142524 - 08/25/15 07:03 PM (8 years, 5 months ago)

Dirty MS syringe


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Offlinetripdawg420
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Re: first monotub a fail ? [Re: tetherface]
    #22142526 - 08/25/15 07:04 PM (8 years, 5 months ago)

:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Offlinetetherface
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Re: first monotub a fail ? [Re: Psilosoulful]
    #22142545 - 08/25/15 07:07 PM (8 years, 5 months ago)

Quote:

Psilosoulful said:
Dirty MS syringe



that's what I figured OP do you have any other spawn colonizing?


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OfflineGrim767
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Re: first monotub a fail ? [Re: tetherface]
    #22142618 - 08/25/15 07:20 PM (8 years, 5 months ago)

Quote:

tetherface said:
at least your getting fruits out of it bro :thumbup: i'm honestly surprised it did that well if there was sludge in the bottom of the spawn you used. Did you ever figure out the source of the bacteria?



Well I Cut the sludge away when I spawned them. The pics don't do the pins et justice. I'm thinking overly wet WBS or too much spore solution or contaminated syringe though I was fairly careful making it SAB ect.


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflineGrim767
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Re: first monotub a fail ? [Re: Psilosoulful]
    #22142627 - 08/25/15 07:22 PM (8 years, 5 months ago)

Quote:

Psilosoulful said:
Nice! Looks like you coulda had a better pinset if it was cased, but not bad. My other suggestion would be to readjust and dial in the polyfill better, I see some fuzzy feet on the bases of those mushies.



Yeah not bad for first mono. I'll be sure to case next one. Do you think not enough FAE?


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflineGrim767
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Re: first monotub a fail ? [Re: tetherface]
    #22142634 - 08/25/15 07:24 PM (8 years, 5 months ago)

Quote:

tetherface said:
Quote:

Psilosoulful said:
Dirty MS syringe



that's what I figured OP do you have any other spawn colonizing?



Yeah a bunch using agar wedges


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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Offlinespacechildo
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Re: first monotub a fail ? [Re: Grim767]
    #22142640 - 08/25/15 07:26 PM (8 years, 5 months ago)

Quote:

Grim767 said:
Do you think not enough FAE?




I think so, considering they're looking a bit thin and you think you may have a contam in your sub and I'd loosen up the top holes.

your fruits can also be skinny because of the contamination but FAE will always help anyway!


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OfflineGrim767
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Re: first monotub a fail ? [Re: spacechildo]
    #22142644 - 08/25/15 07:27 PM (8 years, 5 months ago)

Alright will do thanks for advice


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Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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Offlinespacechildo
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Re: first monotub a fail ? [Re: Grim767]
    #22142648 - 08/25/15 07:28 PM (8 years, 5 months ago)

:thumbup:

good luck with your agar stuff man, got some pics of those new grain jars?


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OfflineGrim767
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Re: first monotub a fail ? [Re: spacechildo]
    #22142700 - 08/25/15 07:38 PM (8 years, 5 months ago)

Yeah the growth in some is going pretty slow think contamed :frown: others going good I'll take some pics for yeah gimme min.


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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Offlinetetherface
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Re: first monotub a fail ? [Re: Grim767]
    #22142713 - 08/25/15 07:41 PM (8 years, 5 months ago)

right on bro that's great your using agar to inoculate now:thumbup: clean spawn is a must to achieve the best results. I have a completely different understanding of "clean" spawn now vs when I just started. I used to think my spawn was clean but it wasn't :facepalm3: it was clean enough to use most of the time, but definitely wouldn't call it clean now.


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OfflineGrim767
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Re: first monotub a fail ? [Re: Grim767]
    #22142809 - 08/25/15 07:58 PM (8 years, 5 months ago)

Ok here's some pint jars I di with agar wedges my plan was once they were full colonized to spawn them to 3-4 quart wbs bags

Growth is stalled in some of the others and I'm pretty sure there's some sort of contamination. Here's some quart jars I did with some agar wedges
they seem to be doing alright.
And here's some I just inoculated with a MS syringe


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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Offlinetripdawg420
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Re: first monotub a fail ? [Re: Grim767]
    #22142836 - 08/25/15 08:02 PM (8 years, 5 months ago)

:takingnotes:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineGrim767
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Re: first monotub a fail ? [Re: tetherface]
    #22142848 - 08/25/15 08:03 PM (8 years, 5 months ago)

Quote:

tetherface said:
right on bro that's great your using agar to inoculate now:thumbup: clean spawn is a must to achieve the best results. I have a completely different understanding of "clean" spawn now vs when I just started. I used to think my spawn was clean but it wasn't :facepalm3: it was clean enough to use most of the time, but definitely wouldn't call it clean now.



Yeah I think my problems are coming from
A. Contamed syringes
B. WBS prep
C. Improper sterile technique
Though I'm using SAB I think I'm just not fluid enough with process and I must be stirring stuff up with the amount I'm moving around in there. Kinda frustrating that I'm still losing jars.


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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Offlinefastlearner
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Re: first monotub a fail ? [Re: tripdawg420]
    #22142852 - 08/25/15 08:04 PM (8 years, 5 months ago)

:stoned:


--------------------
I have no knowledge of any illegal activities that I may or may not have been involved in heard of saw or imagined. Any statements made by me have no basis in scientific facts or real life applications. I've never cultivated mushrooms or any other illegal substance and do not intend to do so ever. I read a book on it once but I'm dyslexic so I probably got shit backwards.


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OfflineGrim767
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Re: first monotub a fail ? [Re: fastlearner]
    #22152279 - 08/27/15 04:11 PM (8 years, 5 months ago)

Here's another update pinset was great considering no casing...


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflinePsilosoulful

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Re: first monotub a fail ? [Re: Grim767]
    #22152283 - 08/27/15 04:12 PM (8 years, 5 months ago)

:aweyeah:


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OfflinetaGyo
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Re: first monotub a fail ? [Re: Psilosoulful]
    #22155918 - 08/28/15 10:41 AM (8 years, 5 months ago)

:vibin:


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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineGrim767
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Re: first monotub a fail ? [Re: taGyo]
    #22171373 - 08/31/15 09:23 PM (8 years, 4 months ago)

So last update I got total of little over 7 oz off first flush which I think is phenomenal I think the tub is spent though I dunked and put back into fruiting conditions and have about 5 mushrooms coming up all in all I'm very glad I talked to you guys and didn't toss my tub lol


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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OfflinePsilosoulful

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Re: first monotub a fail ? [Re: Grim767]
    #22171406 - 08/31/15 09:30 PM (8 years, 4 months ago)

Good job!!! Enjoy those mushies man! :pipesmoke:


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OfflineGrim767
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Re: first monotub a fail ? [Re: Psilosoulful]
    #22171557 - 08/31/15 10:01 PM (8 years, 4 months ago)

Thanks I plan to :smile: couldn't have done it without help from this awesome community


--------------------
Trade List

I won't bow to something that I've never seen,
Can't believe in something that doesn't believe in me,
I'm not blood of your blood,
I'm no son of your god,
I have no faith in your faith,
Still I find salvation.


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Invisibletahoe
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Re: first monotub a fail ? [Re: Grim767]
    #22172364 - 09/01/15 02:36 AM (8 years, 4 months ago)

I would take a spray bottle and mist down that fluffy mycelium.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

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I need to proofread


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