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OfflineLlamaLicker
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Registered: 05/01/15
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Question on jar to jar transfers
    #22090891 - 08/14/15 11:58 AM (8 years, 5 months ago)

Hi there. I was wondering what the truth was behind jar to jar transfers. Specifically,  is there a certain number of generations that one can do this before it starts to degrade the yield or potency. As an example,  if a person used one colonized jar to inoculate ten others,  then used one of those to inoculate ten more and so on, how many iterations can this continue for before issues start to present themselves and what would those issues be? Thanks for your help.


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InvisibleMad Season
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Re: Question on jar to jar transfers [Re: LlamaLicker]
    #22091059 - 08/14/15 12:44 PM (8 years, 5 months ago)

The general rule of thumb is 3 expansions before senescence kicks in. Besides you'd want to keep your cultures young anyway. I've seen someone go as far as 7 before it all just stopped growing.

Agar however you can go like 20+ transfers because you're not shaking it up, and it's not expanding anywhere near as much.


Edited by Mad Season (08/14/15 12:45 PM)


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