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OfflineDankbud420
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No growth of mycleium after 3 days?
    #22089258 - 08/13/15 09:22 PM (8 years, 5 months ago)

I odered a golden teacher spore syringe for sporestore.com (I know, bad mistake but didnt read the reviews till after I ordered). I got it in and it had plenty of spores in it, unlike what most reviews have said. I used the pf-tek method and pc cooked the jars for a hour and let them cool 6 or 7 hours. I didnt use a glove box when inoculation but I used my bathroom which I cleaned and sprayed with disinfectant 5 min before had. I used .5 cc in each jar hole (two holes in the jar) and flamed the needle untill cherry red before I inoculated each jar. After 3 days of setting on my dresser at about 72° I see no mycleium growth in the jars (I inoculated 11). Should I be worried? I dont see any contaminants either so I dont think thats the case. Any help is appreciated, thanks.


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OfflineBoomBoom
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22089280 - 08/13/15 09:32 PM (8 years, 5 months ago)

Reallllly need to look into making a sab man they are cheap and much better than winging it in open air in your doom room. I wouldn't worry until about six to seven days in, it could take that long depending on conditions strain and temp.


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Offlineblasteroid
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Re: No growth of mycleium after 3 days? [Re: BoomBoom]
    #22089325 - 08/13/15 09:46 PM (8 years, 5 months ago)

I've done a successful open air inoc. for 4 jars, took about a week and a half for spots of mycelium to start showing up. Just relax and go knock up a bunch more jars, that usually relieves my anxiety :smile:


Edited by blasteroid (08/13/15 09:47 PM)


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Invisiblewowimflabbergasted
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Re: No growth of mycleium after 3 days? [Re: blasteroid]
    #22089339 - 08/13/15 09:53 PM (8 years, 5 months ago)

Patience :levitate:


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Offlinebluegill
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Re: No growth of mycleium after 3 days? [Re: wowimflabbergasted]
    #22089811 - 08/14/15 01:42 AM (8 years, 5 months ago)

It's only been 3 days. If it grew that fast it would be cobweb mold. Be patient.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: bluegill]
    #22091900 - 08/14/15 04:19 PM (8 years, 5 months ago)

I checked again today and theres growth in one jar. Its a fuzzy dull white spot about the size of a quater. Its got 3 or 4 tiny black spots in it but I cant tell if its vermilculite or not.


Edited by Dankbud420 (08/14/15 06:14 PM)


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Offlineinked4life
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22092011 - 08/14/15 04:58 PM (8 years, 5 months ago)

usually takes at least 4 days before u see germination but sometimes it can take longer. depends on how old the spores are, conditions in your substrate and plenty of other things can account for the extra time.  This hobby requires patience, i give any jar a month to show signs and after that i chuck em. if the black spreads dont even open them in ur house. black mold is dangerous for ur project and your health.


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22092012 - 08/14/15 04:59 PM (8 years, 5 months ago)

Pics!


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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22092049 - 08/14/15 05:07 PM (8 years, 5 months ago)

just be patient plus at 72 degrees there going to go a little slower. try to get the temp up to the high 70s 77, 78 something like that just dont go above 80


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Re: No growth of mycleium after 3 days? [Re: Chimaira]
    #22092079 - 08/14/15 05:17 PM (8 years, 5 months ago)

Actually 65-75 is best. You won't see anything slowed down until the low 60s, or even lower.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22092219 - 08/14/15 06:12 PM (8 years, 5 months ago)

It wont let me upload pics from my gallery on my phone, how do I upload them?


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22098962 - 08/16/15 02:25 PM (8 years, 5 months ago)



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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22098968 - 08/16/15 02:26 PM (8 years, 5 months ago)



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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099096 - 08/16/15 03:07 PM (8 years, 5 months ago)

im pretty sure you got some mold growing there.

try to take some better pics but the fact that you had nothing, then a quarter size spot the next day and now this the day after tells me thats not cube myc.


you should build a SAB for sure even though its possible to get away without it for brf jars you will have better chances with one. and your bathroom is the dirtiest place in your house so thats not a good place to use even if you did spray it with lysol.

also you want to take the foil off the top of the jar after you take them out of the PC. your dry verm barrier works just fine for catching nasty's but if you keep foil on the lid the idea is it may cause your verm barrier to become damp and ineffective


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Offlineinked4life
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Re: No growth of mycleium after 3 days? [Re: Mdahmer]
    #22099190 - 08/16/15 03:30 PM (8 years, 5 months ago)

that dont look good sorry to say, dont look like it would smell good either. usually if growth is fast and off white its cobweb or something else. i can clearly see where the spore germinates from in my jars. there isnt an orginating germination spot in that jar


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Re: No growth of mycleium after 3 days? [Re: inked4life]
    #22099237 - 08/16/15 03:46 PM (8 years, 5 months ago)

:shrug: it doesn't look terrible to me. Bad lighting on the 1st, and the 2nd looks good to me.. I mean the foil should be taken off, but if those were mine, I'd let em ride a few more days.

I'd also highly recommend making more jars as you wait


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Re: No growth of mycleium after 3 days? [Re: inked4life]
    #22099289 - 08/16/15 04:02 PM (8 years, 5 months ago)

knocked up on the 1st


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: inked4life]
    #22099481 - 08/16/15 04:52 PM (8 years, 5 months ago)

Day 6


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099482 - 08/16/15 04:53 PM (8 years, 5 months ago)



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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099488 - 08/16/15 04:53 PM (8 years, 5 months ago)

Much better lighting. That looks good :thumbup:


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099497 - 08/16/15 04:55 PM (8 years, 5 months ago)



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Offlineinked4life
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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22099502 - 08/16/15 04:56 PM (8 years, 5 months ago)

yeah much different with better lighting, ur good its just fuzzy mycelium


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: inked4life]
    #22099514 - 08/16/15 04:59 PM (8 years, 5 months ago)



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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099525 - 08/16/15 05:01 PM (8 years, 5 months ago)



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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099543 - 08/16/15 05:05 PM (8 years, 5 months ago)

You're good so far :P


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099548 - 08/16/15 05:06 PM (8 years, 5 months ago)

that spot looks good. the one above it i think looks suspect though. sometimes its hard to tell in the beginning and your mix seems to have some spots of clumpy brf which can also look a little odd in the pics.

when you make the substrate try to really mix it well with a fork so its all uniform


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Mdahmer]
    #22099830 - 08/16/15 06:17 PM (8 years, 5 months ago)

Sounds good, if it is mold how long will it take for it to show?


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22099862 - 08/16/15 06:24 PM (8 years, 5 months ago)

well if its cube mycelium it will eventually start looking like that other one you have. it will thicken up kinda. i mean it may still just be the picture i dont know some people seem to think its fine and maybe it is.

if it starts to turn any other colors or if it grows real fast and is more grey than white you will know its bad.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Mdahmer]
    #22103201 - 08/17/15 03:26 PM (8 years, 5 months ago)

Is it ok to take the lid off and smell them? If so what would a normal jar smell like and what would a contaminated one smell like?


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Offlineinked4life
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22103260 - 08/17/15 03:42 PM (8 years, 5 months ago)

i wouldnt advise in opening, i noticed trich in my dry verm layer, opened it up and scooped what i thought was it and today more appeared in the dry verm. dont open unless ur willing to sacrifice it. i figured it just one jar so i can afford the loss.


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Re: No growth of mycleium after 3 days? [Re: inked4life]
    #22103294 - 08/17/15 03:51 PM (8 years, 5 months ago)

I really don't advise on opening. You can try to smell through your injection holes.. Whatever's there will stay there though, dry verm layer will hold it in. I always smell when I'm birthing or spawning. Because like said before.. once you open it, it's done.


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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22103319 - 08/17/15 03:56 PM (8 years, 5 months ago)

same exact situation man! I inoc'd 5 jars in my Lysol'd down tiny bathroom about a week and a half ago, and today I saw growth in 3 of them! Same thing happened with my last grow too


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: blew42]
    #22103340 - 08/17/15 04:01 PM (8 years, 5 months ago)

blew42 did yours get contaminated?


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22103363 - 08/17/15 04:06 PM (8 years, 5 months ago)

I really want to try a LC with a spore print but dont want to spend 30 bucks on a print and I cant access the market place for any free ones bc I havent been registered long enough


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22103386 - 08/17/15 04:14 PM (8 years, 5 months ago)

You do not want to try LC with a spore print.  You want to put spores to agar, clean up the culture, then put that into an LC so that you're not dropping bacteria and mold spores in sugar water.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Inocuole]
    #22103761 - 08/17/15 05:52 PM (8 years, 5 months ago)

So its best to do LC with a syringe?


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22104788 - 08/17/15 10:26 PM (8 years, 5 months ago)

Quote:

Dankbud420 said:
blew42 did yours get contaminated?




No, so far no contams. My last grow was with 2 pint sized ziplock tubs and I did the same open air style inoculation and had a fairly successful yield with no contams.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: blew42]
    #22106556 - 08/18/15 12:19 PM (8 years, 5 months ago)

Oh ok lol, I thought you ment they got contaminated. Sounds good though


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22106631 - 08/18/15 12:41 PM (8 years, 5 months ago)

Quote:

Dankbud420 said:
So its best to do LC with a syringe?




It's best to draw the LC with a syringe but you shouldn't ever inoculate LC with a syringe of anything, except maybe other verified clean LC.

Never put spores from a print, syringe, or otherwise into LC if you can help it.  If you can't help it and do it anyway then you'll probably come to understand why that's not recommended.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Inocuole]
    #22107326 - 08/18/15 03:51 PM (8 years, 5 months ago)

I shouldnt have to make a LC now, all jars look to have cube mycelium growing


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112534 - 08/19/15 03:27 PM (8 years, 5 months ago)

So today I looked and some have great mycelium growth and some have mold. Will post pics in a few mins but is a jar with green mold ok to keep? Ive heard its non toxic and instead of throwing it out you can let the mycelium take over instead, is that true?


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112568 - 08/19/15 03:33 PM (8 years, 5 months ago)

No, throw them away.


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: wowimflabbergasted]
    #22112714 - 08/19/15 03:58 PM (8 years, 5 months ago)



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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112719 - 08/19/15 03:59 PM (8 years, 5 months ago)

Lipstick or fusarium. Toss.


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112730 - 08/19/15 04:02 PM (8 years, 5 months ago)

3 are clean,1 has the green mold and  7 have the red colored stuff, should all be throwed away?


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112731 - 08/19/15 04:02 PM (8 years, 5 months ago)

Just the moldy ones. Not the ones with no signs of things yet.


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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22112745 - 08/19/15 04:04 PM (8 years, 5 months ago)

On the pic on the one with the green mold, the white in the bottem is mycelium right?


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112757 - 08/19/15 04:08 PM (8 years, 5 months ago)

Quote:

Dankbud420 said:
So today I looked and some have great mycelium growth and some have mold. Will post pics in a few mins but is a jar with green mold ok to keep? Ive heard its non toxic and instead of throwing it out you can let the mycelium take over instead, is that true?



No


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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112764 - 08/19/15 04:09 PM (8 years, 5 months ago)

Yessir, on the bottom to the left of the green is cube myc. Have you thought about trying agar? It seriously saves ass


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22112793 - 08/19/15 04:13 PM (8 years, 5 months ago)

This is my first time so I just wanted to start out simple, going to be trying a few different things next time


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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112801 - 08/19/15 04:14 PM (8 years, 5 months ago)

Really sucks that im going to lose 8 jars though :frown:


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InvisibleMad Season
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112823 - 08/19/15 04:18 PM (8 years, 5 months ago)

That's why I said to try out agar :lol: and it's no harder than making jello. In fact that's pretty much what it is, and what agars used for. Honestly I think everyone should just start out with agar. If that stuff was on an agar dish you can just cut out the clean myc. As seen here:
http://www.shroomery.org/forums/showflat.php/Number/18430998


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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OfflineDankbud420
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22112843 - 08/19/15 04:22 PM (8 years, 5 months ago)

Can I put this stuff in my compost bin or would the mold spread and start sucking the nutrients out of it? And from what ive seen agar is pretty pricy.


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InvisibleMad Season
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Re: No growth of mycleium after 3 days? [Re: Dankbud420]
    #22117140 - 08/20/15 02:59 PM (8 years, 5 months ago)

I always just throw my agar in my contam pile :shrug: it eventually just dries up and flakes away.

Agar's really no more expensive than grains. Though I have a good source. A really sweet Asian market.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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InvisibleInocuole
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Re: No growth of mycleium after 3 days? [Re: Mad Season]
    #22117656 - 08/20/15 05:11 PM (8 years, 5 months ago)

I throw my contamed agar in a bush outside. :shrug:


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OfflinetaGyo
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Re: No growth of mycleium after 3 days? [Re: Inocuole]
    #22118038 - 08/20/15 06:35 PM (8 years, 5 months ago)

I throw my contamed agar in my neighbors basement :shrug:


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