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MushLover2552
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Registered: 08/13/15
Posts: 33
Last seen: 7 years, 6 months
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Back at it! (Mushlover's Monotub grow, including grain slurrying) 1
#22087188 - 08/13/15 01:30 PM (8 years, 5 months ago) |
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First time returning to cultivation in a year or two, glad to be back!
I noticed as I went to 'noc some jars up today that quite a bit of WBS was burst in each jar, as well as a little sticky/clumpy (threw 2 out, was able to shake the other 6 into separation)
Used the rest of my syringe which is no big deal, but wondering if i should get on the fast track to ordering a new one seeing how the state of my jars are... any opinions? no pooled water in jars, just a little starchy/sticky and did around 1-2ml solution each.
Burst grains... 
Let's try that again...
 a little better!

Took one of those, blended about 200ml colonized WBS with 400ml water, and poured into 12 more


Going strong!!
99% of the way there!

Turned those guys into a nice tub, 1 brick coir, 2.5qts fine verm, 1/3 cup gypsum mixed into boil, 6.5 - 7qts spawn used

Edited by MushLover2552 (09/15/15 07:40 PM)
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Eesh those don't look too good.
Keep them and see what happens, but you should definitely get into agar if you are doing grain.
I'll send you a print so you don't have to buy another syringe, but I won't be able to send it out till tomorrow, if you like.
Use this link list as your resource. There is a ton of good info here
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MushLover2552
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Registered: 08/13/15
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Last seen: 7 years, 6 months
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Really appreciate the response and print offer, nate!! :-)
I do have a wild print from QLD I can play around with, got a little discouraged from spore -> agar since they don't seem to want to germinate for me (same story a couple years ago). I'm relatively experienced with agar but I much prefer live tissue transfers 
Gonna cook up another batch in a day or so and see how it goes, I think the problem is my shitty Presto canner vents a little too much and it was running low on water at about 60 out of the 75-90 mins @ 15/psi
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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maybe you just need to turn the heat down to medium or something so that the weight only joggles a couple times a minute.
The spores from your wild print may not be viable now. You could try pressurized hydration as done here by Stro.
If you decide you want that print just send me a PM
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shroomyaxn



Registered: 11/13/11
Posts: 276
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I switched from WBS to rye. Rye are more consistent in size. I also have had less burst grains when using rye.
Whether you are using agar or MS to inoculate, you still need to get the grain prep right.
I soak my rye for 12-18 hours in hot tap water, then boil for 5-10 mins ONLY so I can steam dry the rye. Steam drying takes me 10-15 mins.
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MushLover2552
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Registered: 08/13/15
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Last seen: 7 years, 6 months
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Soaked WBS Sunday into Monday, PC'd, then nocc'd up with MS on Tuesday, as of today 3/5 jars have healthy growth and I suspect the other 2 will follow soon Tossed the old 6, still no growth and they were just too far gone.. Last batch was also the last of the discontinued Pennington Economy mix I had, used Pennington WBS Classic for the new generation which is a little bit more @ $16 for 40lbs but a really nice mix high in millet nonetheless. If all 5 of these turn out, the plan is to turn one into a master for 7-8 more jars and the other 4 will be going into a mono for some cloning opportunities >:D
On another note, does anyone with a flowhood or a small closet setup do periodic cleanings? I have a flowhood in a very small closet, in between uses would it be worth doing my normal airscrub routine (lysol, closet down, turn on hepa 20 mins later and wait an hour or two before doing work) or not bother? Will update with pics/progress as it comes along, looking forward to sharing! Thanks nate and shroomy, both reducing boil time + heat for the PC helped tremendously, still a couple grains SLIGHTLY opened up but that's because I overboiled slightly again, no problem it seems!
Edited by MushLover2552 (08/23/15 07:55 PM)
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BlueIndian
Maestro



Registered: 01/17/10
Posts: 858
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My advice after just starting some rye....is don't use WBS anymore...trust me it's worth the extra cost. Go by RR's tek and it is sooo much easier...no mess...don't have to worry about burst seeds or being overly wet.
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nooberst
Stranger



Registered: 03/03/15
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Or try oats haven't failed me yet
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MushLover2552
Stranger

Registered: 08/13/15
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Last seen: 7 years, 6 months
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Re: Burst WBS jars [Re: nooberst]
#22133059 - 08/23/15 09:59 PM (8 years, 5 months ago) |
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Unfortunately there's no local source/sellers of ryeberries in my area, I'd have to resort to paying $1/lb plus shipping, maybe in the future a solution will come forth but for now i'll have to hang onto WBS
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Continue growing those if you have already noc'ed them as you say. They are not ideal, but they will do the trick.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Quote:
MushLover2552 said: Unfortunately there's no local source/sellers of ryeberries in my area, I'd have to resort to paying $1/lb plus shipping, maybe in the future a solution will come forth but for now i'll have to hang onto WBS
Hwve yu tried oats like nooberst said?
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MykoMyers
RASTASCLEPIUS


Registered: 10/09/14
Posts: 154
Loc: West Coast
Last seen: 6 years, 3 months
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Why is there so much water in that jar ??
-------------------- Reishi for trade
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MushLover2552
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Registered: 08/13/15
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Last seen: 7 years, 6 months
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Which jar, MM? Could be a camera/lighting illusion, none of my jars have visible /pooling water besides maybe some unevaporated spore solution?
I have not yet tried oats but I may give em a try if there's any benefits over WBS. It's a bit early to tell but I think I've pretty much got the prep dialed in with this newest batch of jars, just a couple tweaks needed 
So when my plates come in, should I wait until I have some fruits to clone or can/should i just throw a few seeds out of the master onto some agar and transfer til clean etc?


My little bernzomatic torch above^ no lab use yet but I tried it out on the scalpel and HOLY SHIT is it so much faster than the alcohol wick torch.. 2-3 seconds and completely red hot versus 10-15 seconds trying to hold it steady on the wick :P
Edited by MushLover2552 (08/25/15 02:27 PM)
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Those look nice. Seed is blown but we know that. Did I miss something? Is something contaminated?
Go ahead and use some colonized grain for the agar if you want. But wait till the jar is colonized. Then you could also clone once you get fruits. I see no reason why you shouldn't finish the grow. Unless I am missing something
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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MykoMyers
RASTASCLEPIUS


Registered: 10/09/14
Posts: 154
Loc: West Coast
Last seen: 6 years, 3 months
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There was a jar from the original post which had water in it . Actually your right it was like the 3rd post it was lined up with something in the background and it appeared like it was filled with water
-------------------- Reishi for trade
Edited by MykoMyers (08/25/15 05:22 PM)
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MushLover2552
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Registered: 08/13/15
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Last seen: 7 years, 6 months
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nothin wrong with these new 5, tahoe, the ones in original post are all exploded and got tossed :P
Goin good so far... winner of these jars is gettin' slurried into 20 more!

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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Those look great. Now shake the shit out of them
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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kosmokratorshaman
Cosmic Creator


Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
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Re: Burst WBS jars [Re: tahoe]
#22159233 - 08/29/15 03:08 AM (8 years, 5 months ago) |
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I definitely need to make lids like that.
-------------------- I am.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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I have been using lids like that minus the injection port for twenty years. They work great. Just recently been using filter disc. If it ain't broke, don't fix
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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MushLover2552
Stranger

Registered: 08/13/15
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Last seen: 7 years, 6 months
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Re: Burst WBS jars [Re: tahoe]
#22159543 - 08/29/15 07:48 AM (8 years, 5 months ago) |
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You don't think it's too early to shake em, tahoe?
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MykoMyers
RASTASCLEPIUS


Registered: 10/09/14
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Loc: West Coast
Last seen: 6 years, 3 months
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Definitely time to shake
-------------------- Reishi for trade
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Quote:
MushLover2552 said: You don't think it's too early to shake em, tahoe?
To early? Nope! Too late maybe if there were such a thing. Should have shook them 3 days ago.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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MushLover2552
Stranger

Registered: 08/13/15
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Re: Burst WBS jars [Re: tahoe]
#22179258 - 09/02/15 05:47 PM (8 years, 4 months ago) |
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4 outta 5 goin' strong! last one was a little slow to recover (and perhaps not colonized enough) but I'll be giving her another shake tomorrow night 
blender is in and these should be 100% within 24hrs, time to prep a shitload of grain!

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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Maybe give them all one more good shake. Surely shake ole number 4. And at least shake one of the others for comparison
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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dowtish
biological disaster



Registered: 08/16/15
Posts: 150
Loc: Middle TN
Last seen: 6 years, 2 months
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Re: Burst WBS jars [Re: tahoe]
#22180459 - 09/02/15 09:29 PM (8 years, 4 months ago) |
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Kind of thrilled to see this thread. I just did my WBs prep yesterday and I feel like I went a bit over. Got sticky and maybe a few burst grains, but no water pooling. Hoping they do well, but am about to prep again this weekend just in case.
-------------------- So crucify the ego, before it's far too late To leave behind this place so negative and blind and cynical, And you will come to find that we are all one mind Capable of all that's imagined and all conceivable. Just let the light touch you And let the words spill through And let them pass right through Bringing out our hope and reason ...

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MushLover2552
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Re: Burst WBS jars [Re: dowtish]
#22183507 - 09/03/15 03:15 PM (8 years, 4 months ago) |
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Looks like I might have a touch of bacillus.. gonna take a whiff of it after i shake + open before seeing if i wanna spawn or do anything with it..
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dowtish
biological disaster



Registered: 08/16/15
Posts: 150
Loc: Middle TN
Last seen: 6 years, 2 months
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Boo!
-------------------- So crucify the ego, before it's far too late To leave behind this place so negative and blind and cynical, And you will come to find that we are all one mind Capable of all that's imagined and all conceivable. Just let the light touch you And let the words spill through And let them pass right through Bringing out our hope and reason ...

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MushLover2552
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Last seen: 7 years, 6 months
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Re: Burst WBS jars [Re: dowtish]
#22201009 - 09/07/15 08:44 AM (8 years, 4 months ago) |
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Spoke too soon, they're all at 100% now and I don't see anything out of the ordinary Did a grain slurry to 12 jars yesterday morning, seeing recovery in all 12!

and a peek at the workspace

not the most ideal but it works great in a pinch
Edited by MushLover2552 (09/07/15 02:05 PM)
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dowtish
biological disaster



Registered: 08/16/15
Posts: 150
Loc: Middle TN
Last seen: 6 years, 2 months
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Hell yeah man! Good news for sure. Excited for you.
I have some jars that I thought were a loss because of too much heat and stickyness, but they seem to be colonizing fairly well. Hopefully all mine will get to 100%..we'll see.
Explain to me this slurry process if you dont mind.
-------------------- So crucify the ego, before it's far too late To leave behind this place so negative and blind and cynical, And you will come to find that we are all one mind Capable of all that's imagined and all conceivable. Just let the light touch you And let the words spill through And let them pass right through Bringing out our hope and reason ...

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MushLover2552
Stranger

Registered: 08/13/15
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Last seen: 7 years, 6 months
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Re: Burst WBS jars [Re: dowtish]
#22212764 - 09/09/15 03:22 PM (8 years, 4 months ago) |
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Update!
Threw a bunch of jars into this extra tote to keep the workspace tidy, plus I don't like to lysol directly around/onto my polyfil filters..
12 colonizing, 7 with foil that need to be nocc'd, and 6 quarts WBS in the PC along with 400ml distilled water still cooling off for a slurry

7 of the most impressive currently, other 5 are a little behind but should take over quickly enough This is about 80 hours after inocculation

and the donor jar for round 2 of slurrying

Dowtish, grain slurry consists of water + colonized grain spawn, I use an oster blender with an ice blade attachment to knock mycelia off of the grains, then pour a bit of water from the slurry into fresh jars 
eatyualive has a huge thread on it, 3 day colonization time etc.
Edited by MushLover2552 (09/10/15 05:39 PM)
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dowtish
biological disaster



Registered: 08/16/15
Posts: 150
Loc: Middle TN
Last seen: 6 years, 2 months
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Thanks man! I'll look it up.
Looking good man! Im excited for you!
-------------------- So crucify the ego, before it's far too late To leave behind this place so negative and blind and cynical, And you will come to find that we are all one mind Capable of all that's imagined and all conceivable. Just let the light touch you And let the words spill through And let them pass right through Bringing out our hope and reason ...

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MushLover2552
Stranger

Registered: 08/13/15
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Last seen: 7 years, 6 months
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Re: Burst WBS jars [Re: dowtish]
#22224600 - 09/11/15 10:11 PM (8 years, 4 months ago) |
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Definitely check it out and let me know what you think, dow!
7 outta 12 of my round 1 slurries will be fully colonized in less than 24hrs, 5 others within 48 hours.. 5 days for 100% isn't too bad for MS!
As for round 2 jars, I'm seeing recovery in all 12, even if very slight. Most noticeable 2

Spawning a monotub tomorrow using damions coir tek, 6 or 7 qts spawn and half cup gypsum!
got some black bags for monos, more lysol, coir bricks, and also 3 ziploc pp5 containers for agar work 
Edited by MushLover2552 (09/12/15 06:59 AM)
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MushLover2552
Stranger

Registered: 08/13/15
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Should I fruit these bastards yet? 9 days since spawning, 2 more colonizing now.

 ^ don't mind the water droplets
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dowtish
biological disaster



Registered: 08/16/15
Posts: 150
Loc: Middle TN
Last seen: 6 years, 2 months
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Looks ready! Do it!
-------------------- So crucify the ego, before it's far too late To leave behind this place so negative and blind and cynical, And you will come to find that we are all one mind Capable of all that's imagined and all conceivable. Just let the light touch you And let the words spill through And let them pass right through Bringing out our hope and reason ...

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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Burst WBS jars [Re: dowtish]
#22276415 - 09/22/15 09:15 PM (8 years, 4 months ago) |
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nice job. what grain slurry ratios of water to grain were you using?
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MushLover2552
Stranger

Registered: 08/13/15
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Last seen: 7 years, 6 months
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thanks dow, poly'd em up and set a couple cfl's above
eat, i used a 1:2 grain to water ratio  i noticed the blender doesn't actually blend any of the wbs up, just knocks tons of mycelium off into the water, is this working as intended?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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yeah its like a shroom shake. your doing a very low ratio. ive been doing 2:1 grain to water. but thats really fast colonization! you may be pouring a bit more per jar than me though. ill only do a small dash about a tablespoon's worth per jar.
if you use the higher grain ratio it will blend up the grain into a muddier type mix. but what your doing looks to be working well. and i wouldn't change it if your getting results like that. it appears to be the GLC type slurry. I feel the GLC type can expand the most at the quickest rate.
its no more difficult than doing g2g to me. and ill use a shmuvbox with a 55 gallon bag on it. i can comfortably fit 50 quarts in there and pour 1 pint grain slurry that will inoculate 50 plus. its much easier to do it in one sitting. whereas, i use Liquid inoculation from plates and i find myself having to do multiple sessions to accomplish the same end goal. so it ends up being more work, takes more time. but i like both methods. you should check this out. the leap off is amazingly fast if you have plates. i like the grain slurries because depending on the situation if you only have one quart left, you have enough inoculum for a large amount of quarts with little effort. g2ging won't expand the mycelia that far in that time frame.
i have a feeling if your liking the grain slurries, this is something between a pf jar slurry and a LI from a regular agar plate. i look at it depending on the situation. if i have plates ready to go, doing a t3 transfer from a regular agar plate to a cornmeal plate is easy. you can then just flip the jar upside down right into your blender jar, blend and pour. also take a look in this link at carcase-x's tribal jar. its a badass build that makes pouring super easy with the use of a magnet on the pour jar. its a blender and pour jar built into 1.
http://www.shroomery.org/forums/showflat.php/Number/22252599
did you shake the grain slurry after a few days of growth or just let it colonize on its own? you don't normally have to shake them unless you have a really poor shake after inoculation. ive had some really crappy ones colonize only the top or bottom halves of jars. just me rushing to get things done. its always good to take a little extra time to shake each jar to get a good even distribution of mycelia.
Edited by eatyualive (09/22/15 10:06 PM)
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