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Offlinedigdog
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Red Wet Spots
    #22087173 - 08/13/15 01:29 PM (8 years, 5 months ago)

Can anyone tell me what this is?



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InvisibleMr.GuessWork
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Re: Red Wet Spots [Re: digdog]
    #22087272 - 08/13/15 01:38 PM (8 years, 5 months ago)

Those are all bacterial colonies (the red and white mucus-like spots). The plate is littered with them. If you're trying to isolate the mycelium, then cut a chunk of the fungus out and put it on a clean plate and wait for it to grow past the bacteria, and repeat until none of those spots show up. If you do a grain transfer from that plate, then you'll transfer the bacteria that is hidden under the mycelium.


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Offlinedigdog
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Re: Red Wet Spots [Re: digdog]
    #22087296 - 08/13/15 01:43 PM (8 years, 5 months ago)

@Mr.GuessWork

Thank you for your response. That is what I figured. I have another plate that is growing cleanly n so I will just pitch that one.

DigDog


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InvisibleMr.GuessWork
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Re: Red Wet Spots [Re: digdog]
    #22087378 - 08/13/15 02:06 PM (8 years, 5 months ago)

It would take maybe a couple transfers to get a clean plate out of that one if you want to play around with it for fun. If you've got a microscope handy, those bacteria are kind of cool to look at you'd probably need somewhere around 1000X magnification to see them well.


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Offlinedigdog
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Re: Red Wet Spots [Re: Mr.GuessWork]
    #22090520 - 08/14/15 09:51 AM (8 years, 5 months ago)

Yeah I have a decent microscope but I don't want to play around too much because I am kind of a slobbish klutz anyway so I don't want to spread it around. I already threw the sample into a bucket of bleach water, I want that vermin to die!! lol


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Offlinedigdog
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Re: Red Wet Spots [Re: digdog]
    #22115787 - 08/20/15 08:48 AM (8 years, 5 months ago)

@Mr.GuessWork

I found another plate (from same batch / inoculation day) that showed tiny little mucus and pink spots. That is a plate I don't want to lose so I took your advice and cut out sections of the mycelium where it looked clean and transferred them out to several new plates.

One of the new plates burst out in pink overnight! All the others are clear so far. Will be interesting to see how the other plates do. If I get just one clean on out of it I will be happy.

Damn bacteria doing what it is designed to do! lol


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InvisibleMad Season
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Re: Red Wet Spots [Re: digdog]
    #22116676 - 08/20/15 01:10 PM (8 years, 5 months ago)

Have you thought about using antibacterial agar when you transfer this particular one? You have no idea how annoying hidden contams are. They've ruined tons of people's stuff, so be thorough!


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InvisibleMr.GuessWork
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Re: Red Wet Spots [Re: Mad Season]
    #22122788 - 08/21/15 02:32 PM (8 years, 5 months ago)

Quote:

digdog said:
@Mr.GuessWork

I found another plate (from same batch / inoculation day) that showed tiny little mucus and pink spots. That is a plate I don't want to lose so I took your advice and cut out sections of the mycelium where it looked clean and transferred them out to several new plates.

One of the new plates burst out in pink overnight! All the others are clear so far. Will be interesting to see how the other plates do. If I get just one clean on out of it I will be happy.

Damn bacteria doing what it is designed to do! lol




If the whole plate broke out like that over night, then you're probably transferring a significant amount of bacteria into your plate somehow, or if you had just prepared the agar prior to pouring, then you might want to suspect that the agar is contaminated. You also might want to make sure the plates don't have any condensation or water rolling around in them. Moving water on the surface of agar spreads contams around like crazy.

I'm glad the other plates are working though. I always fuck up at least one plate.


Quote:

Mad Season said:
Have you thought about using antibacterial agar when you transfer this particular one? You have no idea how annoying hidden contams are. They've ruined tons of people's stuff, so be thorough!




What kind of antibacterial agent is used in the agar? I'd be scared that some resistant litter bugger would survive the antibacterial agar without notice, and then contaminate a jar. I kind of like the idea of seeing that no contaminants have grown and knowing that it's because there are no contaminants present.


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InvisibleMad Season
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Re: Red Wet Spots [Re: Mr.GuessWork]
    #22122832 - 08/21/15 02:41 PM (8 years, 5 months ago)

It definitely isn't bomb proof. But it is a great way to bring down bacteria (after a few transfers) you'd otherwise not see. Like this one:


Quote:

Kizzle said:

Sneaky bacteria




Just because it looks clean, doesn't mean there's no bacteria under it. A few antibacteral transfers, and even a hot pour would go a long way. And if it contaminates after all that work, it just wasn't meant to be :shrug:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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