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InvisibleKauaiOrca
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Registered: 08/12/08
Posts: 3,131
Agar Recipe Question
    #22086521 - 08/13/15 12:04 PM (8 years, 5 months ago)

I"ve recently gotten a pre mixed agar/LME product that have quite a bit left of that I think is about 50/50 in ratio.  I'd like to experiment with Grain Water / Agar.  I've read that you need a lot less Agar if the grain water is "thick" and was wondering if I can use this Agar/LME for this purpose or will having both LME and Grain Water in the same mixture throw it all off?  Any thoughts?


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"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Agar Recipe Question [Re: KauaiOrca]
    #22086544 - 08/13/15 12:09 PM (8 years, 5 months ago)

the lmea is already everything ya want in an agar:shrug:


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InvisibleKauaiOrca
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Registered: 08/12/08
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Re: Agar Recipe Question [Re: cronicr]
    #22086667 - 08/13/15 12:40 PM (8 years, 5 months ago)

Quote:

cronicr said:
the lmea is already everything ya want in an agar:shrug:




I"ve been reading about the advantages of grain water agar, primarily as it can cause pinning from cultures and those pins can lead to really good clone cultures.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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OfflinecronicrFacebook
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Re: Agar Recipe Question [Re: KauaiOrca]
    #22086688 - 08/13/15 12:45 PM (8 years, 5 months ago)

malt comes from grains and pins just fine as well



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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleStropharis
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Registered: 04/02/07
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Re: Agar Recipe Question [Re: cronicr]
    #22086891 - 08/13/15 01:02 PM (8 years, 5 months ago)

The other day I was reading about endospores and the soak vs no-soak topic. I ended up reading about malt extract production, going on about sprouting grains for enzyme production so essential sugars can extracted, interesting stuff.

OP, :whathesaid:

Use what you have as is, no reason not to. If you want to play around just get some straight agar powder.


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InvisibleKauaiOrca
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Registered: 08/12/08
Posts: 3,131
Re: Agar Recipe Question [Re: Stropharis]
    #22087246 - 08/13/15 01:34 PM (8 years, 5 months ago)

Thanks for the advice.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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