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Offlineuninc4life2010
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Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol?
    #22083916 - 08/12/15 11:05 PM (8 years, 5 months ago)

It seems like it is a tad bit cheaper to buy.  My gut feeling is that it would work fine, since it is just alcohol with a little methanol added.


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Offlinebluegill
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: uninc4life2010]
    #22083941 - 08/12/15 11:12 PM (8 years, 5 months ago)

I would not use denatured alcohol to SANITIZE as in spray or wipedown because it is TOXIC. I would use denatured alcohol for flame sterilization because it burns clean and blue hot.


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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InvisibleStygianKnight
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: bluegill]
    #22083949 - 08/12/15 11:14 PM (8 years, 5 months ago)

Yes, it's a fine replacement for many of your Isopropyl needs.


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InvisibleMad Season
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: StygianKnight]
    #22083976 - 08/12/15 11:20 PM (8 years, 5 months ago)

People still use alcohol? :lol: with a sab all you need is soap. Oh and good techniques. Check out the improve your sterile techniques link in my sig


--------------------
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AMU Q&A
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OfflinecronicrFacebook
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22084165 - 08/13/15 12:32 AM (8 years, 5 months ago)

Quote:

StygianKnight said:
Yes, it's a fine replacement for many of your Isopropyl needs.



:whathesaid:
Quote:

Mad Season said:
People still use alcohol? :lol: with a sab all you need is soap. Oh and good techniques. Check out the improve your sterile techniques link in my sig



i still wipe down my receiving jars and shit..or work drunk:shrug:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineuninc4life2010
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22084554 - 08/13/15 02:33 AM (8 years, 5 months ago)

Quote:

Mad Season said:
People still use alcohol? :lol: with a sab all you need is soap. Oh and good techniques. Check out the improve your sterile techniques link in my sig




I usually spray down my SAB with a 15% bleach solution.  I use alcohol for wiping anything down that goes into my SAB, such as donor and receiving jars, plastic bags containing agar plates, and the gloves I wear while working.  I always assumed alcohol was part of good sterile technique.


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InvisibleStygianKnight
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: uninc4life2010]
    #22084720 - 08/13/15 03:40 AM (8 years, 5 months ago)

I do this as well, as it wipes clean and doesn't leave any slippery residue on jars.


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InvisibleMad Season
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: StygianKnight]
    #22084880 - 08/13/15 04:53 AM (8 years, 5 months ago)

:shrug: I've just not used alcohol or gloves for a long time now, and I haven't noticed a difference :lol:. I wanted to cut back my waste and expenses, and see if it makes a difference. I just wash my hands with soap and water before working, and that's it. No gloves. Even jars and plates going into the box don't generally get wiped. The pc sterilizes everything, including the scalpel, and the dishes I'm transfering from are getting opened using the pop and slide method in my techniques.

This proves to me, that what matters most is the techniques to be able to never let anything hover over the open media. Even if you doused your gloved hands in alcohol, if you go over open media, you're pretty much done. That's what a SAB really is about.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Edited by Mad Season (08/13/15 04:55 AM)


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Offlinespacechildo
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22084891 - 08/13/15 05:00 AM (8 years, 5 months ago)

do you wash jars kept in open air with soap and water before opening them in SAB?
or simply no cleaning of any jars at all before SAB work?


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InvisibleMad Season
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: spacechildo]
    #22084895 - 08/13/15 05:03 AM (8 years, 5 months ago)

Simply no cleaning lol. I've pretty much halted all sanitizers. Except the occasional alcohol wipe down of tubs.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (08/13/15 05:05 AM)


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Invisibleblindingleaf
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22084956 - 08/13/15 05:45 AM (8 years, 5 months ago)

i use lysol for everything except my gloved hands.
but i have a dog, I'm a heavy smoker, and i work outside a lot, so my hands are pretty dirty

do what works for u.  but bleach smell sucks, kudos to whoever can stand that whole mess :lol:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleMad Season
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: blindingleaf]
    #22084965 - 08/13/15 05:49 AM (8 years, 5 months ago)

Haha I'm a mechanic who lives on a farm in an old 70s farm house with horses and 4 dogs. I'm the most unsanitary person I know :lol:. Fuck bleach no lie. I can't stand that shit. Also isn't lysol just alcohol? Lol. I'd prefer lysol. At least it smells way better :lol:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (08/13/15 05:57 AM)


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Invisibleblindingleaf
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22084981 - 08/13/15 05:57 AM (8 years, 5 months ago)

yea, but it leaves a residue on ur jars/plates/etc that standard alcohol doesn't, so i like it cause i can wipe everything I'm using that session, and then go at it w/o stopping to re wipe what I'm using next.

i use a hood too, so any particles on my hands/gloves can get blown in stream.  with an SAB, it makes sense that u can not wipe hands and still have great results!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleMad Season
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: blindingleaf]
    #22084993 - 08/13/15 06:05 AM (8 years, 5 months ago)

Yeah if it was a hood, I'd have the entire surface of my work space, and everything that wasn't pc'd wiped down. Also that's a good bit of info to know about lysol. If I get a hood, I'd definitely use lysol now :smile: :thumbup:! Which I am wanting to get since I want to use spawn bags, and work with edibles..


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisibleblindingleaf
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22085007 - 08/13/15 06:12 AM (8 years, 5 months ago)

if i didn't suck so bad at doing g2g's in an SAB, i would have waited longer to get a hood, but i was using a 66qt tote for SAB, and it was easy for agar and agar>grain, but sucks balls for g2g IME.  seems like people have a better box these days thats taller.  not sure why i didn't catch on to that whole deal :facepalm:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleMad Season
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: blindingleaf]
    #22085026 - 08/13/15 06:23 AM (8 years, 5 months ago)

Yeah dude exactly! It was you who actually recommended me to get a bigger SAB a few months back :lol: I'm glad you did man because I was so fed up with everything. Just moves so much air around.
My 1st sab. :thumbdown: I only use this one for printing nowadays.

after you told me to go bigger. 110 qts baby. Still cheaper than a hood eh :lol:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisibleblindingleaf
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Re: Is it okay to use denatured alcohol for sanitizing purposes instead of isopropyl alcohol? [Re: Mad Season]
    #22085051 - 08/13/15 06:36 AM (8 years, 5 months ago)

haha nice!!

yea, i kinda dropped the ball on the SAB.  live and learn


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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