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Offlinedirttoldfart
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Some growth in month-old jars
    #22082691 - 08/12/15 06:16 PM (8 years, 5 months ago)

I inoculated some jars almost 2 months ago, and after about 3 weeks with no signs of growth I kind of just gave up on them and forgot about them. However, today I glanced at the last few that I haven't gotten around to cleaning out yet, and I noticed some white growth in two of the jars. Here are some pictures of it in one of the jars. I'm wondering, is this some kind of contamination or is there any chance that this is mycelium just growing very late?




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OfflinetaGyo
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22082766 - 08/12/15 06:32 PM (8 years, 5 months ago)

Looks like myc to me :thumbup:

From the top there is kind of weird. Watch it closely, if it spreads like wildfire then that's not good.


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Invisiblechampinhom
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Re: Some growth in month-old jars [Re: taGyo]
    #22083002 - 08/12/15 07:31 PM (8 years, 5 months ago)

2 months? Wow. It must be pretty dry. Well, if they somehow come to fruit some day it will be a little like a 52 year old woman getting pregnant.


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: champinhom]
    #22083449 - 08/12/15 08:59 PM (8 years, 5 months ago)

Do you think it could've been caused by the cakes being too dry? I was always careful of putting too much water in because I had read that too much moisture would lead to contaminations.


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Offlineblazedup
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22083609 - 08/12/15 09:43 PM (8 years, 5 months ago)

When inoculating your jar did you see the liquid run down the side of the jar or possibly end up getting a lot of it in your verm layer


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Offlinebluegill
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Re: Some growth in month-old jars [Re: blazedup]
    #22083809 - 08/12/15 10:40 PM (8 years, 5 months ago)

I would have re-inoculated after a week or two with no signs of growth. Your jars have some really wispy odd looking growths but I think they are mycelium, too.


--------------------
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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: blazedup]
    #22083831 - 08/12/15 10:44 PM (8 years, 5 months ago)

Yep, saw it running down the down the side of the jar, but it is possible that some of it could have gotten into the dry verm layer a bit in a couple of the jars. Do you think this would cause delayed germination? Or maybe that the white growth is not mycelium?


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: bluegill]
    #22083849 - 08/12/15 10:49 PM (8 years, 5 months ago)

I did re-inoculate 6 of the 12 original jars about two weeks after the initial inoculation, but unfortunately I don't know if these two jars were in the re-inoculated group or the other group. If it is mycelium, do you think that there is anything I can do to help it grow healthier?


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Offlinebluegill
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22083964 - 08/12/15 11:17 PM (8 years, 5 months ago)

The best thing you can do to cultivate mycelium is to be patient and just let it happen. Inoculating again was a good call on your part as long as you took sterile measures.

If the inoculant did not make it past the dry verm, yes growth would have been stalled a bit but not much because the water in the solution would have dampened the verm making it easier for the spores to germinate and colonize the sterile sub.


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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Invisibletahoe
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Re: Some growth in month-old jars [Re: bluegill]
    #22084341 - 08/13/15 01:16 AM (8 years, 5 months ago)

I don't think the spores would have germinated of they were just inn the dry vermiculite. Looks like someone made a spore syringe from old spores.
Re shooting pf jars is a good way to contaminate a good syringe. Too risky to stick something good and sterile into a cake that has unknown issues.


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: tahoe]
    #22084468 - 08/13/15 01:59 AM (8 years, 5 months ago)

So are you saying that the white growth would not be mycelium because the spores are too old? Or maybe just that the reason why it took so long to germinate is because the spores are so old?


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Offlineamiablecelebi
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22084591 - 08/13/15 02:47 AM (8 years, 5 months ago)

Can you show us how big the holes in the top of the jars are? Lack of proper gas exchange seems to stunt mycelial growth; at least, in my experience.


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InvisibleMad Season
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Re: Some growth in month-old jars [Re: amiablecelebi]
    #22084957 - 08/13/15 05:46 AM (8 years, 5 months ago)

As spores they don't have mycelial growth. You can germinate on agar with no gas exchange. I agree with Tahoe. Don't reinoculate things. Just make more jars while waiting. Spores do sound old.


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OfflineFractal420
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Re: Some growth in month-old jars [Re: Mad Season]
    #22084961 - 08/13/15 05:48 AM (8 years, 5 months ago)

Holy shit Megadeth is on shroomery

I have to ask, Mad Season, is your username related to the former sponsor The Mad Season? I missed that site, was great while it lasted

Quote:

dirttoldfart said:
I did re-inoculate 6 of the 12 original jars about two weeks after the initial inoculation, but unfortunately I don't know if these two jars were in the re-inoculated group or the other group. If it is mycelium, do you think that there is anything I can do to help it grow healthier?




That is prolly why youre seeing it develop later... Maybe the first time you didnt shake well or could also be the filter thing


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InvisibleMad Season
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Re: Some growth in month-old jars [Re: Fractal420]
    #22084976 - 08/13/15 05:54 AM (8 years, 5 months ago)

Nah no relation. It used to be run by RR and his wife, but since she passed away he shut it down. My name was actually inspired from the 90s grunge band that had a lineup consisting of members from Alice in chains, pearl Jam, and screaming trees


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineFractal420
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Re: Some growth in month-old jars [Re: Mad Season]
    #22084984 - 08/13/15 05:59 AM (8 years, 5 months ago)

I had no idea RR was TMS^

I love alice in chains!

I remember when Layne Staley died and no one gave a fuck. When it was Kurt Cobain... (Why the fuck did i say megadeth? Was mixing Dave Mustaine up with someone else)


Edited by Fractal420 (08/13/15 06:01 AM)


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InvisibleMad Season
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Re: Some growth in month-old jars [Re: Fractal420]
    #22084998 - 08/13/15 06:08 AM (8 years, 5 months ago)

You'd probably love mad season man. It's Layne who's the lead singer, and for some reason the songs in it are way better than AIC. It's a lot more grunge psychedelic, over grunge metal. This was when Layne and others tried to get over heroine with psychedelics. Imo, it's the best, and they only made 1 album :frown: you can see Layne in my signature. He's in the back left.

Also yeah. It's sad what happened to TMS and RR. :frown:


--------------------
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Links upon links of literally EVERYTHING UP TO DATE

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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (08/13/15 06:11 AM)


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OfflineHeydeeohred
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Re: Some growth in month-old jars [Re: Mad Season]
    #22085356 - 08/13/15 08:45 AM (8 years, 5 months ago)

I cared when Layne Staley died...


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OfflinetaGyo
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Re: Some growth in month-old jars [Re: Mad Season]
    #22085442 - 08/13/15 09:10 AM (8 years, 5 months ago)

Quote:

Mad Season said:
As spores they don't have mycelial growth. You can germinate on agar with no gas exchange. I agree with Tahoe. Don't reinoculate things. Just make more jars while waiting. Spores do sound old.




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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: amiablecelebi]
    #22086284 - 08/13/15 11:19 AM (8 years, 5 months ago)

Here's how the holes on the top of the jars look:



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OfflinetaGyo
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22086368 - 08/13/15 11:32 AM (8 years, 5 months ago)

:thumbup:


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22090531 - 08/14/15 09:58 AM (8 years, 5 months ago)

So here's how the same jar is looking now, about 2 days later. Does everything seem to be right?




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Offlineamiablecelebi
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22093094 - 08/14/15 11:42 PM (8 years, 5 months ago)

Pics are a little blurry, but it looks like healthy mycelium to me. :thumbup:


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: amiablecelebi]
    #22093193 - 08/15/15 12:33 AM (8 years, 5 months ago)

Yeah, sorry about the blurry pictures. I'll update with clearer ones when I see any changes.


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OfflineFractal420
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22093412 - 08/15/15 03:25 AM (8 years, 5 months ago)

Looks like clean myc from the pics


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: Fractal420]
    #22094307 - 08/15/15 10:13 AM (8 years, 5 months ago)

Is it common for it to take this long before any visible growth? If not,any ideas on why it took this long?


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OfflinetaGyo
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22095157 - 08/15/15 03:05 PM (8 years, 5 months ago)

Not common but it happens.


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OfflineFractal420
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Re: Some growth in month-old jars [Re: taGyo]
    #22097459 - 08/16/15 03:28 AM (8 years, 5 months ago)

^didnt you knock it up twice? Did you shake the syringe? How much did you use per jar?


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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: Fractal420]
    #22098343 - 08/16/15 11:06 AM (8 years, 5 months ago)

Yup, did it twice. Shook the syringe and used ~1 mL per jar


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OfflineFractal420
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22101056 - 08/16/15 11:42 PM (8 years, 5 months ago)

Very strange. Didnt penetrate the filter the first time maybe?


--------------------
Dreaming of That face again.
It's bright and blue and shimmering.
Grinning wide
And comforting me with it's three warm and wild eyes.

Prying open MY third eye



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Offlinedirttoldfart
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Re: Some growth in month-old jars [Re: Fractal420]
    #22101855 - 08/17/15 08:26 AM (8 years, 5 months ago)

Possibly. I also steam sterilized because I don't have a pressure cooker, so maybe that could somehow be responsible. However, this morning there seems to be a new issue. I woke up and noticed this yellow/brownish discoloration in the mycelium.




What could be causing this, and is there any way to go about fixing it?


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OfflinetaGyo
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Re: Some growth in month-old jars [Re: dirttoldfart]
    #22101995 - 08/17/15 09:26 AM (8 years, 5 months ago)

No way to fix it, that's the beginning of what looks to be a bad contamination. You can wait it out or toss but I'm telling you now it's most likely garbage can tek time.


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Offlinespacechildo
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Re: Some growth in month-old jars [Re: taGyo]
    #22102605 - 08/17/15 12:24 PM (8 years, 5 months ago)

yeah that's toast. and its not because of the lack of PC, brf cakes are steam sterilized in a normal pot for 90mins.
www.mushroomvideos.com just do exactly what he does and if that fails upload pics for confirmation to go ahead and blame the syringe!


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