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The Stretch Effect
Stranger

Registered: 08/12/15
Posts: 20
Last seen: 8 years, 4 months
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coffee agar?? is that a thing?
#22082059 - 08/12/15 03:56 PM (8 years, 5 months ago) |
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Hello All! 
This is my first time trying to isolate a strain from a syringe on agar. I am going to use spent coffee grounds for my substrate. Does anyone think it would be remotely beneficial for me to add instant coffee to my agar recipe since I'm trying to find a strain that grows best in coffee grounds? Or, if I find a super rhizomorphic stain I can just assume it will grow well in any substrate?
im all about the scientific method, and more than willing to set up a controlled experiment to find out if it makes a difference... I'm just trying not to reinvent the wheel, or waste my time here.
thanks guys
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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In ratios like 1:20 to the other dry ingredients it probably won't do any harm but you don't want it very strong. I haven't tried this in particular but it could result in a quicker leap from the agar to the intended substrate, or it could promote genetics to pin on the agar that like coffee, so you can take a clone straight from the plate and use that on a coffee substrate.
It could also stall it for whatever reason. Adding it in a very small ratio will at very least keep it from actually wasting your time.
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paperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
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Re: coffee agar?? is that a thing? [Re: Inocuole]
#22082183 - 08/12/15 04:23 PM (8 years, 5 months ago) |
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I've been using the grain water that I boil my oats in for agar. It's probably about 20% coffee which I dilute in half again for agar.
-------------------- Why should we strive with cynic frown To knock their fairy castles down? ~ Eliza Cook It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 2 hours
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Somebody told me to try 10% coffee agar for my Mexicana sclerotia making shrooms. Does anyone have a recipe or way to add this to your usual mix? Cant find one anywhere
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: coffee agar?? is that a thing? [Re: Edmunter]
#23822020 - 11/11/16 05:59 AM (7 years, 2 months ago) |
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I would just... use some coffee in place of some of the water. Maybe like 1/8 of the water. But that's just me.
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Edmunter
Mr



Registered: 05/01/13
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Re: coffee agar?? is that a thing? [Re: Inocuole]
#23822161 - 11/11/16 07:21 AM (7 years, 2 months ago) |
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Gotcha.......
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 1 hour, 4 minutes
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Re: coffee agar?? is that a thing? [Re: Edmunter]
#23822211 - 11/11/16 07:45 AM (7 years, 2 months ago) |
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I have done it with success( in a very small ratio) just added like a pinch to each plate. Same with brf. Both did fine
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: coffee agar?? is that a thing? [Re: Edmunter]
#23822322 - 11/11/16 08:55 AM (7 years, 2 months ago) |
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Quote:
Edmunter said: Somebody told me to try 10% coffee agar for my Mexicana sclerotia making shrooms. Does anyone have a recipe or way to add this to your usual mix? Cant find one anywhere
You have the recipe already 10%
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 30 days
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Re: coffee agar?? is that a thing? [Re: bodhisatta]
#23822648 - 11/11/16 11:03 AM (7 years, 2 months ago) |
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we're talking regular strength brewed liquid coffee right?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: coffee agar?? is that a thing? [Re: tombosley8]
#23822663 - 11/11/16 11:10 AM (7 years, 2 months ago) |
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Then they would say use 10% of 50% coffee or 10% of 10% coffee would they not?
And at that point they may as well say use 5 or 1% then
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 19 days, 2 hours
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Re: coffee agar?? is that a thing? [Re: bodhisatta]
#23825000 - 11/12/16 06:26 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: Then they would say use 10% of 50% coffee or 10% of 10% coffee would they not?
And at that point they may as well say use 5 or 1% then
Like 10% speedos
So im making a cup of coffee and adding 50 ml in to 450 ml water
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: coffee agar?? is that a thing? [Re: Edmunter]
#23943685 - 12/20/16 02:41 PM (7 years, 1 month ago) |
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So EDmunter did your sclerotia stall out on coffee agar? I'm about to make a batch of agar that I'm going to use for both P. mexicana Jalisco and some Golden Teachers as well. Just wondering if I should screw with making "coffee agar". It seems like it might skew the pH to me.
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 19 days, 2 hours
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Ive had some slow culture but im upping my game with some pins fro an old plate. Get some more coffee culture going soon. I will up date
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: coffee agar?? is that a thing? [Re: PlantManBee]
#23943729 - 12/20/16 03:04 PM (7 years, 1 month ago) |
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Quote:
PlantManBee said: So EDmunter did your sclerotia stall out on coffee agar? I'm about to make a batch of agar that I'm going to use for both P. mexicana Jalisco and some Golden Teachers as well. Just wondering if I should screw with making "coffee agar". It seems like it might skew the pH to me.
I recently made some coffee agar for my tamps and CN with a bit of MEA and diluted liquid coffee...the 2 differences I noted were: 1)Slower growth (possibly due to the additional nutrients or lower pH, when compared to 3% MEA) 2)Retardation or lack of formation of the dark hard material that develops under the mycelial surface.
Here's a shot showing the difference in growth rates...very minor. If anything, I would say the particular recipe I used for my stone producers is more of a hindrance than a benefit.
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: coffee agar?? is that a thing? [Re: Mycolorado]
#23943755 - 12/20/16 03:16 PM (7 years, 1 month ago) |
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Thanks y'all! Since this will be my first DIY batch I suppose I should Quote:
Mycolorado said:
Quote:
PlantManBee said: So EDmunter did your sclerotia stall out on coffee agar? I'm about to make a batch of agar that I'm going to use for both P. mexicana Jalisco and some Golden Teachers as well. Just wondering if I should screw with making "coffee agar". It seems like it might skew the pH to me.
I recently made some coffee agar for my tamps and CN with a bit of MEA and diluted liquid coffee...the 2 differences I noted were: 1)Slower growth (possibly due to the additional nutrients or lower pH, when compared to 3% MEA) 2)Retardation or lack of formation of the dark hard material that develops under the mycelial surface.
Here's a shot showing the difference in growth rates...very minor. If anything, I would say the particular recipe I used for my stone producers is more of a hindrance than a benefit.

Thanks y'all! Since this will be my first DIY batch I suppose I should fault toward the tried and true.
I think I read somewhere in these threads that sclerotia weren't particularly a great mix with malt in the plates. But I could easily have misread and/or remembered this.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: coffee agar?? is that a thing? [Re: PlantManBee]
#23943774 - 12/20/16 03:21 PM (7 years, 1 month ago) |
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TMC says stone production is highest on 4.5% MEA grown in the dark. I've got stones forming on a few 3% MEA plates grown in the dark. Here are a few transfers:

My tamp and CN thread: https://www.shroomery.org/forums/showflat.php/Number/23892271/page/1
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: coffee agar?? is that a thing? [Re: Mycolorado]
#23943800 - 12/20/16 03:38 PM (7 years, 1 month ago) |
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cool! Guess it's going to be MEA plates then.
Thanks again!
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