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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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You need agar agar (I got mine from ebay), glad mini rounds, potato flakes, some light malt karo or honey, micropore tape, and a PC. Check out Pasty's tek, that's where most people start when they dip their toes into agar.  http://www.shroomery.org/forums/showflat.php/Number/19208976
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shellzenone
Zen



Registered: 12/11/14
Posts: 688
Last seen: 1 year, 8 months
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Just wondering OP, did you use metal lids? I have found that sometimes rust particles end up dropping down to the LC. Im thinking about switching to plastic lids for this reason.
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Quote:
Psilosoulful said: You need agar agar (I got mine from ebay), glad mini rounds, potato flakes, some light malt karo or honey, micropore tape, and a PC. Check out Pasty's tek, that's where most people start when they dip their toes into agar.  http://www.shroomery.org/forums/showflat.php/Number/19208976
I was doing research all morning, but I found a buddy who just happened to have some shroomsupply MaltExtract Agar lying around, can I use this with petri dishes (the first recipe on here is for same thing http://www.shroomery.org/5243/What-are-some-good-agar-media-recipes). How would I use this? It says it needs to be sterilized, do I sterilize with water added after or water added before sterilization? Do I sterilize it in a quart jar and then transfer to petri dishes or do I put in petris during PC?
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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I've never done the poured agar tek before, but you are supposed to sterilize your agar mix in a glass beaker with tin foil (loosely) over the top for about 30 min at 15 psi, then once the PC cools down, take out your jar and when it feels comfortable to hold but not too hot that it burns your hand, pour the agar into your petri dishes (in a SAB of course). Put the lids on the petris and let it cool down in the SAB overnight.
Add the water to your agar recipe beforehand, that's what makes it liquid agar. When it cools down, it will become gel-like and ready to use.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Don't use a beaker for pouring agar. You want something with a smaller opening. Like an empty liquor bottle. Or a Pyrex media vessel. I mean, you could use a beaker if you want, but it's not the best option. Hell, there are people that do it with qt jars.
Add 500ml of water to a pot and put it on the stove and get it hot, but not boiling. Then slowly add your required volume of premade agar mixture while stirring constantly. If you are using a non premixed agar, add all dry ingredients slowly and stir them in before you begin adding the agar agar. Once all your dry ingredients including the agar agar have been added and dissolved into the pot of hot water, pour that then into your glass agar vessel(liquor bottle) with a funnel.
I have a 1/4" hole drilled into the lid of my cuervo bottle with a sfd patch on top of it, which I then cover with foil. After so many uses the thread on the cap for the cuervo bottle have gone shitty and its pretty loose so the filter doesn't do much. When I use my pyrex media vessels I just leave the cap 1/4 turn loose. If you do not have a flowhood, it is preferable to have a filter of some sort on the agar vessel. As your PC cools it will draw in outside air(unless you have a sterilizer that seals and pulls vacuum) this will get drawn into your agar vessel if there is no filter. Before I upgraded my PCs to sterilizers I would move my PC in front of my flowhood for cool down.
Once the PC has cooled enough and there is no pressure, open it up and take out your agar bottle(I do all this in front of a flow hood). Once the agar has dropped below 130F(checked with an infrared thermometer) I begin pouring the plates in front of the hood. I like to stack the empty petris in stacks of 5 all stacked on top of each other in an offset fashion. When I begin pouring I take 5 off the top of the stack and place it next to the stack and begin pouring into the bottom dish of the 5 by lifting the lid and picking up the stack of 4 above. After every 5 dishes I grab the next 5 and place it on top of the stack of 5 that were just poured and repeat.
Here are some pics:

Note the offset stacking of the unpoured dishes in this pic and the stack of poured dishes:

Agar is hot coming out of the PC, be careful:

It probably takes about 90 seconds to pour a whole sleeve of 20 plates.
Quote:
DottoreWolfe said:
Quote:
Psilosoulful said:
Better yet, start your LC with a piece of clean agar next time, I'm assuming you used MS..
Nope, doing a spore print straight to LC, because the vendor only had the print of the exotic strain. http://www.shroomery.org/forums/showflat.php/Number/5238137 The beginner friendly method. Fingers crossed though.
Spore print inoculations are multispore. And prints to LC are risky, so are syringes to LC. The only LC inoculation I would recommend is an agar wedge, or in the case of sclerotia forming species transfering a stone from agar to LC. That tek you linked is ancient and IMHO very flawed.
Edited by Juiceh (08/12/15 04:18 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Did i ruin my LC jars? [Re: Juiceh]
#22082389 - 08/12/15 05:13 PM (8 years, 5 months ago) |
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Re: Did i ruin my LC jars? [Re: bodhisatta]
#22083204 - 08/12/15 08:08 PM (8 years, 5 months ago) |
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Thanks a ton guys hopefully at least one of these LCs produces a clean agar.
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,588
Loc: Up Over
Last seen: 1 month, 13 days
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Just be patient, you'll know soon enough. You can test it on a small grain jar, but i never tested any LC because I'm still yet to have one fail...
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