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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Did i ruin my LC jars?
#22079164 - 08/11/15 10:51 PM (8 years, 5 months ago) |
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Greetings guys. So I was preparing some jars for LC and had finished PCing. Just now I looked inside the jars and saw that I must not have cleaned the jars thoroughly, can I still use these or should I redo it. Theres like a few random brown (I think cardboard, because most of these are unused jars, but could be something else) small clumps (comparable to size of spores in a syringe).
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
Edited by DottoreWolfe (08/11/15 10:57 PM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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If you sterilized the jar long enough, you shouldn't have a problem with some random particles in the water. It might make it harder to tell what is a contam and what is not, once the mycelium starts colonizing the LC, but you should be fine either way, just look carefully under a bright light. Then transfer the healthy mycelium out of the jar.
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Quote:
Psilosoulful said: If you sterilized the jar long enough, you shouldn't have a problem with some random particles in the water. It might make it harder to tell what is a contam and what is not, once the mycelium starts colonizing the LC, but you should be fine either way, just look carefully under a bright light. Then transfer the healthy mycelium out of the jar.
This is my first attempt ever using a PC and making LC I PCd for 35ish minutes with 15psi
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Yep, that's more than enough time to sterilize a LC jar, you're good to go!
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GerG
Ghost



Registered: 05/27/11
Posts: 50
Loc: From Paris With Love
Last seen: 6 years, 8 months
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Careful with LC. That shit can look great, but end up being totally useless. Make sure to do a test jar or two before you start noc'ing up multiple jars.
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"All you know about me is what I sold you." - Maynard James Keenan
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Did i ruin my LC jars? [Re: GerG]
#22079234 - 08/11/15 11:07 PM (8 years, 5 months ago) |
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Better yet, start your LC with a piece of clean agar next time, I'm assuming you used MS..
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Quote:
Psilosoulful said:
Better yet, start your LC with a piece of clean agar next time, I'm assuming you used MS..
Nope, doing a spore print straight to LC, because the vendor only had the print of the exotic strain. http://www.shroomery.org/forums/showflat.php/Number/5238137 The beginner friendly method. Fingers crossed though.
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
Edited by DottoreWolfe (08/12/15 01:34 AM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
DottoreWolfe said:
Quote:
Psilosoulful said:
Better yet, start your LC with a piece of clean agar next time, I'm assuming you used MS..
Nope, I wish, I'm being stupid and doing a spore print straight to LC, because the vendor only had the print of the exotic strain. http://www.shroomery.org/forums/showflat.php/Number/5238137 The beginner friendly method. Fingers crossed though.
Spore prints are never completely contam free, since the fruits are grown in open air. And transferring that to an LC jar, only gives the contams a chance to thrive in a nutrient rich environment. However, there are some growers that have pulled it off and gotten healthy contam free mycelium, but the chances are still pretty low. If you can, I'd suggest getting some agar supplies, following pasty's agar tek, then testing the mycelium from your LC out on the agar dishes FIRST, before going to grains.
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Quote:
Psilosoulful said:
Quote:
DottoreWolfe said:
Quote:
Psilosoulful said:
Better yet, start your LC with a piece of clean agar next time, I'm assuming you used MS..
Nope, I wish, I'm being stupid and doing a spore print straight to LC, because the vendor only had the print of the exotic strain. http://www.shroomery.org/forums/showflat.php/Number/5238137 The beginner friendly method. Fingers crossed though.
Spore prints are never completely contam free, since the fruits are grown in open air. And transferring that to an LC jar, only gives the contams a chance to thrive in a nutrient rich environment. However, there are some growers that have pulled it off and gotten healthy contam free mycelium, but the chances are still pretty low. If you can, I'd suggest getting some agar supplies, following pasty's agar tek, then testing the mycelium from your LC out on the agar dishes FIRST, before going to grains.
I don't get how the chances good be so low if in the tek this guy successfully does it everytime, I mean isn't it basically the exact same as using MS into a LC?
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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On prints its basically more concentrated. You have x amount of contams per centimeter.
If you used square centimeter and dilute it in a 10cc syringe you've spread the contams few and far.
So when you use a drop or two you have a good chance of not getting a contam in the drop
If you scrape that same square directly into an lc then if there was even 1 contam your lc is fucked.
Make sense?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Re: Did i ruin my LC jars? [Re: maddchef]
#22079328 - 08/11/15 11:34 PM (8 years, 5 months ago) |
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Quote:
maddchef said: On prints its basically more concentrated. You have x amount of contams per centimeter.
If you used square centimeter and dilute it in a 10cc syringe you've spread the contams few and far.
So when you use a drop or two you have a good chance of not getting a contam in the drop
If you scrape that same square directly into an lc then if there was even 1 contam your lc is fucked.
Make sense?
Fuck...did I just waste 7 LC jars. Or is there actually a solid chance of one or two surviving.
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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GerG
Ghost



Registered: 05/27/11
Posts: 50
Loc: From Paris With Love
Last seen: 6 years, 8 months
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Re: Did i ruin my LC jars? [Re: maddchef]
#22079366 - 08/11/15 11:43 PM (8 years, 5 months ago) |
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Quote:
I don't get how the chances good be so low if in the tek this guy successfully does it everytime, I mean isn't it basically the exact same as using MS into a LC?
Absolutely nothing works EVERY time. Everything, every method, every tek will fail at some point.
I hate to admit it, but I use LC alot. Even though I am as sterile as possible during every step, I still only have around a 75-85% success rate with LC. And I still don't believe that is an acceptable success rate. Like I said; test everything before using it on anything (except said test jar).
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"All you know about me is what I sold you." - Maynard James Keenan
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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You may not see it in your LC jar once the mycelium starts forming, that's why I suggested testing it out on an agar dish because any contams present WILL show up!! Agar is easy to make and is a necessary tool in getting a healthy culture to work with.
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Quote:
Psilosoulful said: You may not see it in your LC jar once the mycelium starts forming, that's why I suggested testing it out on an agar dish because any contams present WILL show up!! Agar is easy to make and is a necessary tool in getting a healthy culture to work with. 
Instead of testing in agar can I just inoculate one rye grain jar with LC from each of my LC jars. Then wait to see which jars develop contams and then just not use those LC jars?
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
Edited by DottoreWolfe (08/12/15 12:20 AM)
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GerG
Ghost



Registered: 05/27/11
Posts: 50
Loc: From Paris With Love
Last seen: 6 years, 8 months
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Quote:
Instead of testing in agar can I just inoculate one rye grain jar with LC from each of my LC jars. Then wait to see which jars develop contams and then just not use those LC jars?
Yes.
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"All you know about me is what I sold you." - Maynard James Keenan
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
DottoreWolfe said:
Quote:
Psilosoulful said: You may not see it in your LC jar once the mycelium starts forming, that's why I suggested testing it out on an agar dish because any contams present WILL show up!! Agar is easy to make and is a necessary tool in getting a healthy culture to work with. 
Instead of testing in agar can I just inoculate one rye grain jar with LC from each of my LC jars. Then wait to see which jars develop contams and then just not use those LC jars?
Testing is testing
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mossystone14
Stranger
Registered: 08/12/15
Posts: 54
Last seen: 8 years, 5 months
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Re: Did i ruin my LC jars? *DELETED* [Re: bodhisatta]
#22080101 - 08/12/15 06:35 AM (8 years, 5 months ago) |
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Post deleted by mossystone14Reason for deletion: my bad
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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That's because inexperienced cultivators think it is a magic bullet that will save them time or make it easier. It's like giving someone a 1000cc crotch rocket for their 16th birthday. A few kids won't kill themselves but most will.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
DottoreWolfe said:
Quote:
Psilosoulful said: You may not see it in your LC jar once the mycelium starts forming, that's why I suggested testing it out on an agar dish because any contams present WILL show up!! Agar is easy to make and is a necessary tool in getting a healthy culture to work with. 
Instead of testing in agar can I just inoculate one rye grain jar with LC from each of my LC jars. Then wait to see which jars develop contams and then just not use those LC jars?
Honestly, I'd rather test my LC on a small agar dish (less PC time, quicker results) than having to go through the process of testing it on grain jars. Another reason not to go from LC>grain, is that contams can easily hide within a grain jar and not be noticed, whereas contams have nowhere to hide on agar.
Do you really want to spawn grain jars to bulk, only to find out later that they were actually "dirty" spawn jars and your first flush was sub-par or you got trich after full colonization.
There is a reason why agar is HIGHLY recommended by almost every trusted cultivator on this site as a starting point to test your LC's, GLC's, MS, etc...
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DottoreWolfe
Doctor Wolfe


Registered: 02/21/15
Posts: 201
Last seen: 5 months, 12 days
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Darn it! I don't have any agar on hand and money is kind of tight. Is agar agar, water and a pertri dish all I need to test with the agar?
-------------------- Everything I post should be regarded as wholly fictitious or hypothetical, nothing I post has any basis in reality.
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