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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Quote:
Pastywhyte said: Don't take this the wrong way but that looks contamed to hell. If you want to sterilize substrates like poo or straw you can, just don't spawn with em. I do bottle subs a la Muda bottles with poo and the like, get excellent returns with em.

I just read through Muda's Bottle Tek. I think that is the way for me to go. I will have to adapt it here and there but for the most part I can use it as it has been laid out by its namesake. The great beauty of the Muda Tek is it allows you to use a bulk substrate without bulk spawning. Ergo, no need to pasteurize.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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So, it has been three days since I began this experiment. The rye berries used for spawning the grass-manure have regained their white fuzzy demeanor. They look better than ever. But, somehow, they have not been able to communicate any of this to the manure. It has not even begun to be myceliated. It is virgin. Segregation reigns supreme.It's as if the manure is wax coated. If the manure is contamnated, the contamination certainly isn't having any effect on the health of the mushroom fungus growing on the spawn berries.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 6 months
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pasteurizing is not very hard at all and if you do the double bucket its too easy.
you can pasteurize good amounts of poo quickly especially if you have several buckets.
the only time it ever failed me was when i doubled my recipe without upping the water temps so i dont think it got up to temp and everything trich'd out.
search mudafuka and double bucket if you wanna see it explained
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment [Re: Mdahmer]
#22092967 - 08/14/15 10:46 PM (8 years, 5 months ago) |
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Quote:
Mdahmer said: pasteurizing is not very hard at all and if you do the double bucket its too easy.
you can pasteurize good amounts of poo quickly especially if you have several buckets.
the only time it ever failed me was when i doubled my recipe without upping the water temps so i dont think it got up to temp and everything trich'd out.
search mudafuka and double bucket if you wanna see it explained
Thanks for the tip.I found the tek you are talking about. Looks good. I am going to give it a try. I have two of those buckets--don't have a lid but that can be improvised.I will post in this thread my next attempt.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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amiablecelebi
Shrooms Bruh


Registered: 07/08/15
Posts: 39
Last seen: 8 years, 5 months
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If you live near a Wal-Mart, the lids are only $4. I'm just saying that because it's probably better so that it maintains temperature properly.
-------------------- "The fool doth think he is wise, but the wise man knows himself to be a fool." - William Shakespeare
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Quote:
amiablecelebi said: If you live near a Wal-Mart, the lids are only $4. I'm just saying that because it's probably better so that it maintains temperature properly.
I will check that out. I used to be able to get those 5 gallon buckets by the carload for nothing--there was a factory near where I lived at the time that discarded them after emptying them of the chemicals they contained. Probably today that same factory is selling those buckets to Walmart and Homedepot.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment (surprising new development) [Re: champinhom]
#22095378 - 08/15/15 03:54 PM (8 years, 5 months ago) |
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Here is what I posted 18 hours ago:
Quote:
champinhom said: So, it has been three days since I began this experiment. The rye berries used for spawning the grass-manure have regained their white fuzzy demeanor. They look better than ever. But, somehow, they have not been able to communicate any of this to the manure. It has not even begun to be myceliated. It is virgin. Segregation reigns supreme.It's as if the manure is wax coated. If the manure is contamnated, the contamination certainly isn't having any effect on the health of the mushroom fungus growing on the spawn berries.
The above quote is based on what I have been observing in a quart mason jar full of grass-manure that was spawned with colonized rye berries going on 4 days ago. The quart jar was an after thought--most of the grass-manure I had sterilized for my project went into a bread-loaf sized tupperware container together with most of a quart of colonized rye berries. The stuff in the jar is the overflow from the tupperware--it could not hold as much as I had to give it.I then put the tupper in the garage and kept the glass jar in the considerably cooler house. I have been gauging the progress of the colonization/lack thereof by checking what was going on in the jar, which, as my post of about 18 hours ago stated, was basically nada. The rye berries had come back wonderully but had not spread the love to any of the grass-manure.
It was as though the mycelium was holding out its right hand, but the manure was looking the other way.
About half an hour ago I went into the garage for something. While in there, on a whim, I took the tupperware container out of the bin where I'd stowed it and gave it a close look. Lo!, the story it told is completely different from the one being told by the jar. There is a LOT of work being done in that tupper. The grass is getting into the act. The white wave is advancing. I think I may have a winner here after all. I will get a pic of this and post it--my phone is charging even now.
Anyway, I am as surprised as anybody.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
Edited by champinhom (08/15/15 03:55 PM)
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment (surprising new development) [Re: champinhom]
#22095480 - 08/15/15 04:23 PM (8 years, 5 months ago) |
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Here are some pics:

-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment (conclusion) [Re: champinhom]
#22122080 - 08/21/15 11:44 AM (8 years, 5 months ago) |
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This will be the last pic I will be posting on this subject. The Tupperware container is now completely colonized--against all odds!
I think I owe my good fortune to the almost contamination-proof nature of composted grass clipping manure--at least if it is composted in the manner indicated in the OP. Even when sterilized and picked apart bare-handedly it will come through for you--what a guy!
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: an experiment (conclusion) [Re: champinhom]
#22122127 - 08/21/15 12:00 PM (8 years, 5 months ago) |
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Just a note that often contamination will often not show up until you have introduced fruiting conditions. Full colonization is byno means a success to this. You should continue to update until the first flush is in hand.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: an experiment (conclusion) [Re: Pastywhyte]
#22122229 - 08/21/15 12:27 PM (8 years, 5 months ago) |
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Quote:
Pastywhyte said: Just a note that often contamination will often not show up until you have introduced fruiting conditions. Full colonization is byno means a success to this. You should continue to update until the first flush is in hand.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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mustangbob3
Mad Myrmecologist



Registered: 10/15/14
Posts: 1,685
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Re: an experiment (conclusion) [Re: taGyo]
#22122236 - 08/21/15 12:30 PM (8 years, 5 months ago) |
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--------------------
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: an experiment (conclusion) [Re: Pastywhyte]
#22123678 - 08/21/15 05:52 PM (8 years, 5 months ago) |
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Quote:
Pastywhyte said: Just a note that often contamination will often not show up until you have introduced fruiting conditions. Full colonization is byno means a success to this. You should continue to update until the first flush is in hand.
Anything less is a cop out and proves that this doesn't work.
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment (conclusion) [Re: Pastywhyte]
#22124444 - 08/21/15 08:50 PM (8 years, 5 months ago) |
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Quote:
Pastywhyte said: Just a note that often contamination will often not show up until you have introduced fruiting conditions. Full colonization is byno means a success to this. You should continue to update until the first flush is in hand.
Fair enough. I will case this little tub in a few days and see what it can do. Results will be posted here.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment (update) [Re: champinhom]
#22184525 - 09/03/15 07:32 PM (8 years, 4 months ago) |
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So, about 10 days ago I cased my tub of sterilized grass manure spawned with colonized rye berries--the manure being pulled and teased apart with the bare hands of the grower (hands that had been rubbed with 91% alcohol, mind you)and then tumbled up thoroughly with the rye. This thread tells the whole breath taking tale. Anyway, I waited while the casing got taken hold of by the mycelium and then put the tub into a fruiting chamber--really just a tote fitted out like a monotub with a couple of quarts of 10% peroxide water in the bottom. Here is what it looks like 10 days after casing:

It smells as good as it looks.
Here is a pic of a half a quart jar of left over sub--after I had mixed the grass manure and the rye berries together I had too much substrate for the tupperware tub--the pulled apart grass manure got very fluffy and space consuming--so I put the excess in the jar the rye berries had just vacated. This is the same age as the sub in the tupperware, but has not been cased:

This too has an immaculate whiff to it.
So, so far so good. I will update when the flush shows up in force.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: an experiment (update) [Re: champinhom]
#22184547 - 09/03/15 07:34 PM (8 years, 4 months ago) |
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70% isopropyl is significantly better for all sanitation needs. 91% is too anhydrous to effectively permeate cells.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: an experiment (update) [Re: Inocuole]
#22184614 - 09/03/15 07:45 PM (8 years, 4 months ago) |
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Well I won't be recommending this to anyone. . .ever. However I am glad ya got some fruits in the end
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shellzenone
Zen



Registered: 12/11/14
Posts: 688
Last seen: 1 year, 8 months
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Re: an experiment (update) [Re: Inocuole]
#22184697 - 09/03/15 07:58 PM (8 years, 4 months ago) |
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Quote:
Inocuole said: 70% isopropyl is significantly better for all sanitation needs. 91% is too anhydrous to effectively permeate cells.
Interesting, can I dilute 91% with distilled water?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
shellzenone said:
Quote:
Inocuole said: 70% isopropyl is significantly better for all sanitation needs. 91% is too anhydrous to effectively permeate cells.
Interesting, can I dilute 91% with distilled water?
I do
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: an experiment (update) [Re: Inocuole]
#22184832 - 09/03/15 08:24 PM (8 years, 4 months ago) |
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Quote:
Inocuole said: 70% isopropyl is significantly better for all sanitation needs. 91% is too anhydrous to effectively permeate cells.
I have now replaced the 91% alki with the 70%. I must say, an alcohol lamp fueled with 70% is not up to one fueled with 91%.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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