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newshroomer123
Stranger



Registered: 08/06/14
Posts: 378
Loc: Where ever the weed at
Last seen: 2 years, 8 months
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1st grow sterilization questions
#22076003 - 08/11/15 11:26 AM (8 years, 5 months ago) |
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It's my first grow and I'm wondering how extreme I need to go with sterilization. I am planning to wipe down my work area with Lysol wipes, spray disinfectant everywhere, use gloves, one of the face masks that doctors use, wipe down the jars with Lysol wipes before putting in substrate(then give them time to let any moisture evap), make sure there is absolutely no air current in my home and I'm considering flame sterilization between each injection port of every jar, and really leaning towards using a SAB. I'll have 16 1/2 pint jars of b+ and 8 golden teacher. Any other sterilization ideas or things that I'm missing? I'm following the pf tek. I have my verm and brf already mixed in a huge bowl so all I have to do is add water and put it in my jars. Please, any suggestions welcome.
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Cujllickduo



Registered: 06/13/15
Posts: 19,552
Loc: England
Last seen: 3 years, 6 months
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Quote:
newshroomer123 said: It's my first grow and I'm wondering how extreme I need to go with sterilization. I am planning to wipe down my work area with Lysol wipes, spray disinfectant everywhere, use gloves, one of the face masks that doctors use, wipe down the jars with Lysol wipes before putting in substrate(then give them time to let any moisture evap), make sure there is absolutely no air current in my home and I'm considering flame sterilization between each injection port of every jar, and really leaning towards using a SAB. I'll have 16 1/2 pint jars of b+ and 8 golden teacher. Any other sterilization ideas or things that I'm missing? I'm following the pf tek. I have my verm and brf already mixed in a huge bowl so all I have to do is add water and put it in my jars. Please, any suggestions welcome.
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Yerow
Stranger



Registered: 09/22/14
Posts: 1,206
Last seen: 2 years, 7 months
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Re: 1st grow sterilization questions [Re: Cujllickduo]
#22076034 - 08/11/15 11:33 AM (8 years, 5 months ago) |
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wait, you know you need to steam sterilise the jars or pressure cook them right?
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: 1st grow sterilization questions [Re: Cujllickduo]
#22076036 - 08/11/15 11:33 AM (8 years, 5 months ago) |
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when you make the cakes just pretend you're baking something, no need to be clean about anything besides wiping the rim of the jar with a PT before adding the dry verm.
when the cakes are done use a SAB! no need to use injection ports, just inoculate through the paper tape (dont use tape if its not breatheable). flame between each jar at least, each hole will be tiresome!
after the cakes are fully colonized there is no longer any need to keep things sterile. fruiting is done in open air!
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newshroomer123
Stranger



Registered: 08/06/14
Posts: 378
Loc: Where ever the weed at
Last seen: 2 years, 8 months
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Re: 1st grow sterilization questions [Re: spacechildo]
#22076058 - 08/11/15 11:41 AM (8 years, 5 months ago) |
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Yes, I know I am steam sterilizing them. Sorry, everything about sterilization in my post up to "make sure there is no air current..." i planned to do before I steam sterilized. Everything else would be after just to be as close to 100% contam free as possible.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Quote:
taGyo said: Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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