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johnny six-guns
reluctant shaman


Registered: 08/06/15
Posts: 35
Loc: country roads.
Last seen: 8 years, 3 months
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Re: Newbie First Post - G2G stall from lack of moisture? [Re: johnny six-guns]
#22072372 - 08/10/15 04:02 PM (8 years, 5 months ago) |
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And sorry ahead of time to anyone who I come across as a dick to. I am not like that at all, I've just browsed these boards and others alot, and I realize that as a noob what I say or why I do things just makes me seem misinformed or wrong. I just want to say that's not the case.
I know and have done some of the standard ways of doing things, I just ultimately end up mixing them altogether to find my own rhythm of doing things. They're not always as efficient or make sense, but I try to have some reasoning behind them .
If you have any questions about why I am doing something, shoot. Because I'm also always open to suggestions on easier ways. I'm just much more of a troubleshoot and modify type of person than I am a follow the directions type.
-------------------- Cheap - fast - good. Pick any two and it won't be the third.
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johnny six-guns
reluctant shaman


Registered: 08/06/15
Posts: 35
Loc: country roads.
Last seen: 8 years, 3 months
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Re: Newbie First Post - G2G stall from lack of moisture? [Re: blindingleaf]
#22072405 - 08/10/15 04:08 PM (8 years, 5 months ago) |
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Quote:
blindingleaf said: yes, it makes sense. do what works for you.
maybe try cracking the lids on those more. might be a GE issue, because i can't see any bacteria, and i don't think the grains look dry, u have some burst kernels, which means on the whole, they had enough water before autoclave. if i prep rye too dry by accident, it will still leap off and grow, but take a bit longer to get to 100%. the fact that they aren't even fuzzing up makes me think they are not getting enough o2 filters for GE are a pretty standard thing though dude. i would really trust that more than cracked lid. poly, tyvek, etc. when ur jars start getting larger, might be hard to get them to 100%, and because the only entrance for gas is an unfiltered crack, the chance of mold is much higher. if ur getting away with it now, then thats cool, but just know that it is risky and easily avoidable
I'll give that a try right now.
Those 3 jars really perplexed me because they have literally done nothing. I transferred 12 jars lastnight and they're already acting lively and getting to work.
I'm floating around the realm of dry (even though I noticed the burst kernels too), the o2 suggestions you had, or maybe I was very rough on them? After the initial transfer, they were very bruised and blue around the edges, but as you can see that faded. So maybe they're still recovering.
For now I suppose I will check the moisture as well as I can, crack the lids some more, and give it time.
-------------------- Cheap - fast - good. Pick any two and it won't be the third.
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johnny six-guns
reluctant shaman


Registered: 08/06/15
Posts: 35
Loc: country roads.
Last seen: 8 years, 3 months
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Re: Newbie First Post - G2G stall from lack of moisture? [Re: johnny six-guns]
#22072834 - 08/10/15 05:55 PM (8 years, 5 months ago) |
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Thank you spacechildo and blindingleaf.
The culprit turned out to be bacteria, like you guys had said. I was looking for signs of it by wet spots or clumping, or any settled discoloration. While there were no visual signs of any of that in the jars, the nose test let me know that things had gone south.
Now a question of where it came from? I can only assume it was something left over from my substrate. But I thought the long soak and PCing would have taken care of that.
Thanks guy.
-------------------- Cheap - fast - good. Pick any two and it won't be the third.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Newbie First Post - G2G stall from lack of moisture? [Re: johnny six-guns]
#22072867 - 08/10/15 06:03 PM (8 years, 5 months ago) |
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if I'm working with agar and clean plates, and i get bacteria, IME, its either PC time was fucked up and short, or the temps were a little high in the room. if ur using MS syringe, can be that too.
any grain prep will work. but if u start getting bacteria a lot even with clean plates, its good to look at how ur prepping it or how long ur PCing it
if bacteria shows in many places in the jar, up ur PC time. if its around the g2g grains, check ur OG plate, or possibly ur master jar was not PC'd long enough, and did not recover from the shake/g2g
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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johnny six-guns
reluctant shaman


Registered: 08/06/15
Posts: 35
Loc: country roads.
Last seen: 8 years, 3 months
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Re: Newbie First Post - G2G stall from lack of moisture? [Re: blindingleaf]
#22074855 - 08/11/15 04:53 AM (8 years, 5 months ago) |
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After looking at some of my notes, I think I have never the problem down.
I believe those 3 jars were the last of the last batch I had done in my PC before switching to the autoclave. They were done for 75mins at 15 psi. They also look much more full compared to the rest IMO.
There was also a short heat wave through my area right around the time that I transferred those which set the temperature in my house and room inot the high 80s for a couple days.
I think the shorter PC time, coupled with the heat wave and possibly too much innoculating (3 cc of LC) set those jars up for failure.
-------------------- Cheap - fast - good. Pick any two and it won't be the third.
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