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Offlinegator00
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Xanthan gum agar sub?
    #22067283 - 08/09/15 01:42 PM (8 years, 5 months ago)

well I found this and looked it up online and people use agar as a sub for this in cooking so you think it can be done in reverse?


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Anything I say in the above post is a complete lie. The voices in my head tell me to say it. And to burn things. any pictures that are posted are found on google.


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Offlinegator00
memeber over 10 yrs


Registered: 06/17/03
Posts: 262
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Re: Xanthan gum agar sub? [Re: gator00]
    #22067306 - 08/09/15 01:47 PM (8 years, 5 months ago)

well just found this what you guys think???

Curr Microbiol. 2006 Apr;52(4):287-92. Epub 2006 Mar 18.

Xanthan gum: an economical partial substitute for agar in microbial culture media.

Babbar SB1, Jain R.



Author information



Abstract

Xanthan gum, microbial desiccation-resistant polysaccharide prepared commercially by aerobic submerged fermentation from Xanthomonas campestris, has been successfully used alone and in combination with agar for microbial culture media. As illustrative examples, eight bacteria and eight fungi were grown on media solidified with either agar (A, 1.5%), xanthan gum (X, 1%), or combinations of both (0.9% X + 0.1% A, 0.8% X + 0.2% A, 0.7% X + 0.3% A, 0.6% X + 0.4% A). All fungi and bacteria exhibited normal growth and differentiation in all these treatments. Rather, growth of most of the fungi was better on xanthan (alone) and xanthan + agar media than agar medium. As the media gelled with xanthan gum alone flow, it was not possible to incubate Petri plates in inverted position. Moreover, because of the softness, streaking of bacteria was difficult on such media. However, these problems could be overcome by partially replacing xanthan gum with 0.3% agar. Bacterial enumeration studies carried out for Serratia sp. and Pseudomonas sp. by serial dilution and pour-plate method on agar (1.5%), 0.7%/0.6% X + 0.3%/0.4% A yielded similar counts. Selective media, succinate medium for Pseudomonas sp., and MacConkey broth medium for Escherichia coli gelled with 0.7%/0.6% X + 0.3%/0.4% A did not support growth of other bacteria when inoculated along with the above-mentioned bacteria. Likewise, differential medium, CRMA (Congo red mannitol agar) gelled with xanthan-agar combination could differentiate between Agrobacterium tumefaciens and Rhizobium sp.


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Anything I say in the above post is a complete lie. The voices in my head tell me to say it. And to burn things. any pictures that are posted are found on google.


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InvisibleInocuole
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Re: Xanthan gum agar sub? [Re: gator00]
    #22067345 - 08/09/15 01:54 PM (8 years, 5 months ago)

You already have a thread about this. Why are you doing this?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Offlinegator00
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Registered: 06/17/03
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Re: Xanthan gum agar sub? [Re: Inocuole]
    #22067383 - 08/09/15 02:03 PM (8 years, 5 months ago)

its different this isn't about not being able to find agar this is for others to try to find a sub like I said the first one is just looking for other things. yes similar but no not the same


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Anything I say in the above post is a complete lie. The voices in my head tell me to say it. And to burn things. any pictures that are posted are found on google.


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InvisibleInocuole
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Re: Xanthan gum agar sub? [Re: gator00]
    #22067437 - 08/09/15 02:17 PM (8 years, 5 months ago)

Well what if I go and make a thread about what grains I can use for substrate, and then I make another thread right after, about a specific unorthodox grain that I'm thinking about using as a substrate?  So I have "Grain substitutes" and "Can I use sorghum as a grain substitute?"  right next to each other on the first page.

Do you really think this post couldn't have just been included in your other thread?  You actually believe it's justified to make it a separate topic?

:mindblown:

I find that shocking.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Jokes are funny until they're about you


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InvisibleSteveRogers
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Re: Xanthan gum agar sub? [Re: gator00]
    #22067593 - 08/09/15 03:04 PM (8 years, 5 months ago)

Quote:

gator00 said:
well I found this and looked it up online and people use agar as a sub for this in cooking so you think it can be done in reverse?



So what is your question here boss?
Yes that agar recipe is capable of propagating spores from a variety of species.
If you are asking with specific steps on preparation, you are more than likely going to be on your own. This is an atypical recipe using fairly exotic ingredients.
Try it out and report your experiences.

My question to you is, what issues are you having with standard agar recipes? Why did you seek this one out?


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"General, I am loyal to nothing......except The Dream"


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Offlinegator00
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Registered: 06/17/03
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Re: Xanthan gum agar sub? [Re: SteveRogers]
    #22067776 - 08/09/15 03:37 PM (8 years, 5 months ago)

have trouble finding agar and I really cant order online rite now. in that is does say the gum can be used alone and was better then the agae but you just cannot tilt that jar or dish. but im trying to do the pom tek which its gunna sit on top of the pom anyway so I don't think it would matter agar or gum do you?


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Anything I say in the above post is a complete lie. The voices in my head tell me to say it. And to burn things. any pictures that are posted are found on google.


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InvisibleSteveRogers
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Re: Xanthan gum agar sub? [Re: gator00]
    #22067785 - 08/09/15 03:39 PM (8 years, 5 months ago)

now that the pom tek is involved, I am just going to quietly take my leave of this thread. Violet's bullshit is not something I am willing to get involved in.

good luck to you sir.


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"General, I am loyal to nothing......except The Dream"


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