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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: my first inoc... [Re: Mad Season]
    #22068183 - 08/09/15 04:47 PM (8 years, 5 months ago)

exactly what i said relys on good technique too:thumbup:


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InvisibleMad Season
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Re: my first inoc... [Re: mustangbob3]
    #22068201 - 08/09/15 04:49 PM (8 years, 5 months ago)

Tbh it's the main thing a sab relies on lol. You don't need any sanitizers or cleaners if you have a proper technique. I just use soap water. I'm not even wearing disposable gloves anymore lol. I just wash my hands with soap and water.


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Edited by Mad Season (08/09/15 04:50 PM)


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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: my first inoc... [Re: Mad Season]
    #22068226 - 08/09/15 04:54 PM (8 years, 5 months ago)

yes even a flowhood to get best efficency need correct technique.

how you handle ,direction  bag faces all makes a difference in the numbers game.

even how you lift and tilt a lid on a petri,
over hundreds of times will effect your efficency.

and im not even joking

its all in the hand movements, when you got that down your set imo

you can have a SAB and have bad technique and it wont eliminate contam issue altogether.
that require practice not bleach imo


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Edited by mustangbob3 (08/09/15 04:59 PM)


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Offlinespacechildo
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Registered: 01/24/13
Posts: 19,243
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Re: my first inoc... [Re: mustangbob3]
    #22068255 - 08/09/15 05:01 PM (8 years, 5 months ago)

those hand movements dont help much if you dont have a sab or a hood tho.
you can get away with inocing ships and pf-cakes without one but there really is no good reason NOT to use one in this hobby.


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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: my first inoc... [Re: spacechildo]
    #22068284 - 08/09/15 05:06 PM (8 years, 5 months ago)

i said i agree like 4 times lol infact in just about every post!!

but really i dont mind doing it for MS anyway.
i already advised not too lol

im not saying you need hand movement skills for injecting lol

i was refering to increasing the efficiency of a SAB or flowhood by them hand movement skills when doing transfers ect to which i already said i do in a SAB for the same reason you do! :smile:

you guys are miss understanding me lol


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Offlinespacechildo
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Re: my first inoc... [Re: mustangbob3]
    #22068307 - 08/09/15 05:11 PM (8 years, 5 months ago)

OK, just seemed like you thought the SAB was a bunk theory there.
technique is useless without a SAB (etc) and a SAB is pretty much useless without technique! :super:


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InvisibleMad Season
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Re: my first inoc... [Re: mustangbob3] * 1
    #22068356 - 08/09/15 05:21 PM (8 years, 5 months ago)

Seriously bob you're just wrong in everything and fail at life :thumbdown::rofl:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: my first inoc... [Re: spacechildo]
    #22068366 - 08/09/15 05:23 PM (8 years, 5 months ago)

no in every post just about i advocated there use for the exact same reasons:)

and just expressed my opinion that to maximize the efficiency of a SAB takes practice and learning hand movements more than it requires bleach or iso .
water or soapy water is fine as mad said above

but explained why when the odd time i use MS inoccs i dont bother with a SAB.

Quote:

Seriously bob you're just wrong in everything and fail at life :thumbdown::rofl:




ok night lads:laugh2:

EDIT and thermodynamics say that even a sealed tub will not be still air! as it acts like a green house and the temp inside will always be higher than equilibrium of the room.
and any input of heat or any temp differential will mean different densitys and this makes the flows not combine and starts a cyclone effect of heating and rising and cooling and falling
it will be a slowly revolving circle never still

and this same revolving cycle draws in one arm hole and pushes out the other to reach equilibrium with the room.

this  increases also when you put your hands inside as the temp and pressure differential rises quickly.

and if you have a alcohol lamp in there you may as well not even have a SAB the heat will push out air and also draw air in as the oxygen burns.
same if you flame the needle inside sab with lighter you just moved the air(added more potential energy and excited the gas more) more and drew lots new air in.. now you sab is as good as it was before you sprayed down and left it fact.

and lots advise to flame outside the sab not in for this reason and safety

so know matter what you do the air in a SAB is not clean and sterile as inocuoles says.

and neither is it still

air is a gas that means the individual molecules have enough potential energy to push away from their well ordered state of a solid.
they physically move as far away as possible from each other.
x amount move out the holes for this reason and then x amount must re enter.
this happens constantly!

also gas by its very nature will not stay still while in that state and if forced to under pressure becomes a liquid again.:smile:
and we know that is not happening!

come on guys its all basic science from primary education for crying out loud!

not until it has used up it potential energy prescribed by heat!
and then would be liquid and slowly become solid!

science says you are wrong on lots of levels.
SAB are great but your understanding of the mechanics and behaviours is just shit!

read up on thermodynamics and then come and tell me:)

you will see it is impossible unless no heat, no gravity, air composition is pure ect

like i agree SAB rocks if done right and reduces any problems, but seem most dont even understand them to get them most out of them!

especially if you dont even understands the importance of hand movements and tbh finding fault with me when i agree is silly!

you should be jumping all over inocuoles for saying that the air in a sab is sterile not me!

Quote:

If you're familiar with the principles of a still air box, the moisture(iso or water) against the floor and walls of the SAB draws airborne contaminants into it over the course of the 30 minutes you're supposed to let it sit with all your air turned off in the house.  By the time it's run its course there very well should be a pocket of sterile air in there, the rest still being significantly cleaner than open air.




this is straight bollocks of the highest order  and far more harmful to noobs if they listen to this. its just plain wrong lol

but i doubt anyone will say a thing to him.

instead take my words out of context and try to diminish the truth, out of solidarity.
you will find everything i said to be true scientifically and try yourself in open air and you will see the syringes tip hardly ever contams from open air inocs:shrug:

the proof is in the pudding

stop wasting your own time trying to pick faults with my ways!
if you really want truth chase inocuole down he said straight ass crack when he said its sterile in a sab lol far worse than what im saying or have said but seems this goes un-noticed.

seriously you guys looking for a guy to troll?
at least understand the things you are trying to argue!
as all you are doing is demonstrating your lack of understanding and only showing you fell into logical fallacy's.

and used these in a ad hominem way against my opinions that are based on truths not some aunt sally argument you guys have had before when you drew those wrong conclusions!


Edited by mustangbob3 (08/10/15 02:56 AM)


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Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: my first inoc... [Re: Mad Season]
    #22068370 - 08/09/15 05:24 PM (8 years, 5 months ago)

*double post* opps


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Offlinerisky80
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Registered: 07/28/15
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Re: my first inoc... [Re: mustangbob3]
    #22068590 - 08/09/15 06:07 PM (8 years, 5 months ago)

Thanks for the pointer iso first then flame.  I hope i get lucky this time.  What do you think my chances are.  Slim to nil?


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OfflinetaGyo
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Re: my first inoc... [Re: risky80]
    #22068594 - 08/09/15 06:08 PM (8 years, 5 months ago)

Good to nil.

I've seen stranger things happen.

No iso at all. Just flame.


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Offlinebluegill
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Registered: 11/05/13
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Re: my first inoc... [Re: risky80]
    #22068604 - 08/09/15 06:10 PM (8 years, 5 months ago)

There is no way to tell. Just be patient and see.


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"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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Offlineinked4life
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Registered: 06/28/15
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Re: my first inoc... [Re: bluegill]
    #22068710 - 08/09/15 06:27 PM (8 years, 5 months ago)

all my wipes are kept in a container that has 90% alcohol. i have a small room that has no open drafts and nothing is stored or done in there except this. i flame till red hot , wipe the carbon off and inject. i havent had any issues with the way i do it. some choose not to wipe but i found less material sticks or plugs the syringe. not sayin u have to use my process but i never had any contam issues with cakes. i use a sab for most of everything else.


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InvisibleInocuole
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Re: my first inoc... [Re: inked4life]
    #22068724 - 08/09/15 06:30 PM (8 years, 5 months ago)

70% alcohol is better than 90% for sanitization, but neither will kill mold spores.  :shrug:  Do what you want, I'm just putting the facts out there.


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OfflinetaGyo
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Re: my first inoc... [Re: taGyo]
    #22069018 - 08/09/15 07:28 PM (8 years, 5 months ago)

Quote:

inked4life said:
all my wipes are kept in a container that has 90% alcohol. i have a small room that has no open drafts and nothing is stored or done in there except this. i flame till red hot , wipe the carbon off and inject. i havent had any issues with the way i do it. some choose not to wipe but i found less material sticks or plugs the syringe. not sayin u have to use my process but i never had any contam issues with cakes. i use a sab for most of everything else.



The carbon will not affect the spores. Don't wipe the needle.

Quote:

Inocuole said:
70% alcohol is better than 90% for sanitization, but neither will kill mold spores.  :shrug:  Do what you want, I'm just putting the facts out there.



Quote:

taGyo said:
Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.







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InvisibleInocuole
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Re: my first inoc... [Re: taGyo]
    #22069029 - 08/09/15 07:30 PM (8 years, 5 months ago)

If you want to not have carbon all over your needle why not use a torch instead of lighter?  I use a culinary torch, some people use dab torches, torch lighters, etc.  Butane or propane both work fine.  That'll cut your contam rate more than wiping with alcohol ever did, and keep your needles looking clean without the need to ever touch them.  Plus they glow red more quickly.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflinetaGyo
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Re: my first inoc... [Re: Inocuole]
    #22069033 - 08/09/15 07:31 PM (8 years, 5 months ago)

Or alcohol lamp :thumbup:

Torches are faster though, I just like the sciency feel of my alco lamp.


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Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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InvisibleInocuole
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Re: my first inoc... [Re: taGyo]
    #22069044 - 08/09/15 07:32 PM (8 years, 5 months ago)

An alcohol lamp knocked over might as well be a molotov cocktail though.  :shrug:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflinetaGyo
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Re: my first inoc... [Re: Inocuole]
    #22069050 - 08/09/15 07:33 PM (8 years, 5 months ago)

If you lack the coordination to not knock over your alcohol lamp then you probably shouldn't be doing myco work :lmafo:


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