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Offlinechrismcq
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Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS
    #22061749 - 08/08/15 07:54 AM (8 years, 5 months ago)

Hi Guys - My second grow is under way --
All are MS, Pictures are not great but i'll be sure to update with better pics soon.

1 x Z-strain Mini Tub (SGFC)
1 x Z-strain Monotub
1 X Peuto Rican Monotub

My Monotub has a large crack down the middle because of the flex in the tub but from research I hope this wont be a problem.
Knotting has appeared overnight, both have been in fruiting conditions for 3 days. These are Z-strain -



My Largest Monotub is Peuto Rican and i'll be putting this in to fruiting conditions in a few days.
( 4 Cakes Inoculated with Agar mycelium Syringe - 1 print/ 1 clone)



My next Grow I hope to use An agar Isolate or at very least a clean agar wedge transfer.
Im having a few problems with white specks :thumbdown: (bacteria/contam)?
Its LME + Agar Agar Mix and done inside a SAB sealed with Parafilm inside a plastic baggie


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Invisibleblindingleaf
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22061885 - 08/08/15 08:55 AM (8 years, 5 months ago)

try to pick up ur tubs more by the bottom/base, instead of the handles/top, that usually helps me avoid cracking when moving them.

u can easily transfer from that contam, looks more like bacteria than mold.  check ur other plates to make sure they don't have spots before transferring, if they do, up ur PC time for agar by ten minutes.  if they don't, just move a little slower when working with plates in SAB.

good luck mang!


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22062165 - 08/08/15 10:29 AM (8 years, 5 months ago)

Thank's for the advice!
I will lift my tubs from the base in the future and up my PC Time by 10 Mins :thumbup:
A few of my plates have the same bacteria / white spots so the Pc'ing may be the problem :stoned:

(update) First Pin! :grin:



Edited by chrismcq (08/08/15 05:45 PM)


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22066662 - 08/09/15 11:03 AM (8 years, 5 months ago)

Pic Update -


Mini Tub -


The small tub is doing better than expected, The colonisation time was pretty slow and its still looking very brown but it is knotting nicely.
The mix is coir and vermiculite with rye grain, I think i overdid the substrate to spawn mix :ooo: or maybe its the unreliable MS Genetics.

80 New Petri dishes on the way :dancer:
The Clone i took was from a second flush mushroom, Does anyone have experience or knowledge on doing this ? It was large but i've recently tried these mushrooms (same tub) And the potency was disappointing, Im thinking of throwing the clone agar work and stick with the print? I hope to get a good specimen from my current grows and will take a clone from them im the hope to find some potent ones.. I Have took my Peuto rican tub out from colonisation as i've seen small pins showing up. I would love to try PE/APE But its very difficult for me to get hold of here in the UK.


Edited by chrismcq (08/10/15 02:32 PM)


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22072927 - 08/10/15 06:14 PM (8 years, 5 months ago)

Mini tub, Pins are everywhere but the top ?  :crazy:  :rofl:

(Bottom of Tub)


(Side of Tub)


Edited by chrismcq (08/12/15 10:04 AM)


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22080670 - 08/12/15 10:06 AM (8 years, 5 months ago)

Z Strain -




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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22094144 - 08/15/15 09:27 AM (8 years, 5 months ago)

Picked some Z-Strain today :rockon: First Flush MS Grow





Took a Clone of 2 of these - Nice little cluster :thumbup:

Mini tub update -



I've got almost 100% success rate with agar after increasing PC time by 15 mins ( Was 30 - now 45 ) So thanks for the great advice blindingleaf! :bow2: :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


Edited by chrismcq (08/15/15 02:05 PM)


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22101820 - 08/17/15 08:13 AM (8 years, 5 months ago)

Sorry for the bad quality photos -
I'm unsure of what dish to use for my next grow.. can someone please give me a little advice? is this a isolate ?
These are prints from B+ Sectored around 5 times..  Started with a print



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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22102060 - 08/17/15 09:53 AM (8 years, 5 months ago)

I love PR.

:love:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22103003 - 08/17/15 02:28 PM (8 years, 5 months ago)

Me too :cool: Its a great looking shroom and i had good results with them with Cakes.

What do you think of the malabar strain? I Have a syringe and would like to try grow some but am worried it will contaminate due to it being a syringe.. My previous attempt was a malabar cake but turned out contaminated with mycogone ( Likely present in the syringe ) Agar will solve this but Syringe>Agar is very bad, I would love a clone or print makes things much easier..

Is there any way of growing the mycelium on agar without using the sringe solution directly on agar ?
Eg - can inoculate grain or something else and transfer that to agar?

I Got lots of petris and strains currently growing on agar and im going to have to stick with one or two strains for future grows rather than having a ton of overgrown petri's.. Maybe i should invest in a mini fridge :tongue2:
My grow area is small and i cant use any more :crazy: I would love to have more but takes up a lot of space.

I will update photos of my PR Shrooms soon. At the moment its looking clustered and has an uneven pin set but its given me a good clone (hopefully) for my next grow :thumbup:


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103039 - 08/17/15 02:42 PM (8 years, 5 months ago)

be careful with mycogone, that is a white mold, hides easily on top of mycelium.

u can do grain>agar.  i think people do that when they can't get it to germinate on plates from spore solution.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22103222 - 08/17/15 03:31 PM (8 years, 5 months ago)

Thanks! :smile:
Yeah mycogone is a sneaky bastard :lurker:

I'll try MS > Agar once more and hope for better success than last time ( :syringe: Took 20 days to germinate - by that time the plates contaminated ) If this fails grains it is :thumbup:


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103278 - 08/17/15 03:48 PM (8 years, 5 months ago)

Malabar has a tendency to be contaminated out of the syringe so I'd put it on agar. I have a needle I just can't seem to get clean :shrug:


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Invisibleblindingleaf
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103285 - 08/17/15 03:49 PM (8 years, 5 months ago)

try squirting some of ur spore solution onto the plate, maybe 2- drops, then pouring ur warm-hottish liquid agar in the plate.  it will kinda heat up the spores u want (cube), hopefully softening them.  cubes can grow thru 3d matrix easy, but mold has a harder time with that.  usually u will see germination around the edges of the plate.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22103289 - 08/17/15 03:50 PM (8 years, 5 months ago)

:rockon:


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22103506 - 08/17/15 04:44 PM (8 years, 5 months ago)

That's some great advice Thanks!!  :takingnotes: I will try that next time i mix up some agar.

What do you guys recommend when using MEA Agar ?
I Currently use 3 gram LME / 5 Gram Agar Agar 260ml water. I Started off using the normal 5g / 5g / 250ml but felt the agar was a little too solid when set and researching has lead me to believe that less LME / Nutrients = Encourages Rhiz Growth so cut down on them.


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103621 - 08/17/15 05:16 PM (8 years, 5 months ago)

i like more solid agar, so i use 11g or so per 500ml.
but it sucks for spore germination, so i do the spores first>pour.

i never cut nutrients anymore.  yes, it makes things rhizo.  but other than that, not sure what good it ever did me.  but, there are many other growers on here who do it, so theres no wrong way really.  goal should be clean mycelium in any and all cases.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22103682 - 08/17/15 05:31 PM (8 years, 5 months ago)

:whathesaid:

The most annoying thing in the world is cutting nutrients and getting super rhizo myc and then putting it in an LI and throwing it in grains and it immediately turns tomentose as fuck and colonizes slowly. Neither bother me anymore though, it's all about that pinset.


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22106978 - 08/18/15 02:34 PM (8 years, 5 months ago)

Okay thank you guys, I will keep experimenting and do what works best for me :thumbup:
I Will update with some PR Photos tomorrow and will have to take another clone from them.. My first 2 contaminated with bacteria due to being too small, Turned out very difficult to rip the small shroom / pin in half and cut out a small section to transfer.. I can take a larger specimin tomorrow for cloning :smile:


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22110938 - 08/19/15 11:29 AM (8 years, 5 months ago)

PR First Small Shrooms :dancer:




Edited by chrismcq (08/19/15 02:23 PM)


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22115201 - 08/20/15 02:49 AM (8 years, 5 months ago)

First Flush Z Strain was 20Gram Dry.

Second Flush Update --

Picked the first few today.

PR Update -

Loving the Strength / Pin clusters of Puerto Rican !


Got a nice clone fruit here :rockon: Will be taking a tissue sample from this big boy and smaller one in a large cluster.
Would be a great to achieve a monster shroom on the later flushes :crazy2: :thumbup:


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22116167 - 08/20/15 10:39 AM (8 years, 5 months ago)

The large shroom Kept growing and growing without the veil breaking :dancer:
Weighs 9.5 Grams wet, I have cut a tissue sample and put on agar :thumbup:
I Was going to place a drop of malabar spore solution on my dish and then pour the agar like you suggested but before i knew it the agar was poured.. Oh well, Have used 2 x 3 section Petri dishes - a drop in each part, would be nice to get some success.
I Have to many B+ Dishes so have stopped working on that strain and made a B+ Syringe to try 'preserve' it


Edited by chrismcq (08/20/15 01:44 PM)


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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22117083 - 08/20/15 02:45 PM (8 years, 5 months ago)

Good shit bro!

Try to increase FAE a bit. I see myc crawling up the base of the mushrooms which is normally a sign that they could use a little more fresh air.

http://www.shroomery.org/forums/showflat.php/Number/17332777


--------------------
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TNF Q&A :rockon:
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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22117567 - 08/20/15 04:47 PM (8 years, 5 months ago)

Cheers man !  :lol: :thumbup:

More FAE It is - I've Adjusted the polyfill slightly and will try keep the window open at all times :wink:

I Noticed 1 or 2 of the shrooms totally hairy up the stem :crazy: genetics may have played a part on this occasion.


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22117615 - 08/20/15 05:01 PM (8 years, 5 months ago)

Nah, all the way up is definitely FAE :wink:

If it's just at the base then sometimes it's genetic. For instance:



Those get fuzzy feet no matter what I do, you can see it at the bottom there.


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TNF Q&A :rockon:
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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22122152 - 08/21/15 12:10 PM (8 years, 5 months ago)

Ok :wink: Thank you for the Info and example ! :bow2:

Really pissed about my recent agar batch :frown: Checked on them today and its not set as it should..

Its New agar agar so may be poor quality or it was not mixed well enough, Used 10g agar agar / 8g LME / 500ml Water.
I Have tested the agar by using 125ml water and 2.5gram agar and it set okay soo.. :crazy: i'll just have to keep my fingers crossed.
The agar / clone sample wobbles in the petri dish at room temperature :facepalm3:


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Offlinechrismcq
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22151190 - 08/27/15 12:09 PM (8 years, 5 months ago)

Agar Update --

My Malabar MS to agar has started to grow, Its took off quickly but no sign of mycelium  :crazy:


All my agar clones ^ survived :rockon: The mycelium grown well in the LME that did not set.
With some care i have took another transfer today and now all the dishes are perfect :stoned: Will be using them for my next (3rd Grow)

Cant wait to do an agar grow and harvest them all at once and not 3 to 5 at a time :tongue2:

Grow update --

A Little Advice here would be great, I've recently harvest a few mushrooms from my PR Tub and have seen sign of possible contamination.
Could this be Verticillium ? What do you guys think ?




I think I have over misted the tub and its lacked some fresh air so its not looking too healthy but i cant see any signs of contamination. 
Im allowing it to dry out a little and increased FAE.. Any Advice or guidance is greatly appreciated :takingnotes:


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22155808 - 08/28/15 10:23 AM (8 years, 5 months ago)

Not a vert expert but I don't think it is.

Also, Malabar myc looks really weird and for some reason a lot of us have contamed syringes. Don't be so quick to judge :lol:


--------------------
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TNF Q&A :rockon:
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22155857 - 08/28/15 10:30 AM (8 years, 5 months ago)

Yes, the brown spots on your stems is verticillium.


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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: Fantastic Mr. Fox]
    #22155874 - 08/28/15 10:33 AM (8 years, 5 months ago)

Ahh I see it now :yesnod:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: Fantastic Mr. Fox]
    #22156969 - 08/28/15 02:42 PM (8 years, 5 months ago)

Quote:

Fantastic Mr. Fox said:
Yes, the brown spots on your stems is verticillium.



:whathesaid:


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: cronicr]
    #22157988 - 08/28/15 06:59 PM (8 years, 5 months ago)

Thank you for clearing that up guys :thumbup:

Shame about the malabar syringes :frown:
Im pretty sure its not mycelium by the sheer speed of growth, I attempted syringe to agar before and it took me 3+ Weeks to see signs of mycelium, This white contamination only 5 days from inoc.. I have took another transfer anyhow to study and will update with better photos when possible :lol:


Seeing some improved growth from my PR Tub now i've stopped misting and fan more often but nothing picture worthy, My Z - strain tub is almost exhausted (3rd flush) yet my PR are still struggling to pull out its first flush :confused: Keeping my fingers crossed that i've not screwed up the tub.


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22159988 - 08/29/15 10:06 AM (8 years, 5 months ago)

I Found a fungus gnat flying inside the tub and killed it, next day i found another :awedisgust:

I fucked up on this PR tub from the begining with over misting and lack of FAE..
The verticillium set in and then i spoted the gnats :rolleyes:



I did not want to risk future grows with an infestation problem so have picked all the shrooms and thrown out the subs cleaning the area with lysol.



Got a nice clone and hopefully some potent ass tiny shrooms so its not all a loss :grin: Its all Experiance :stoned:


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22159994 - 08/29/15 10:07 AM (8 years, 5 months ago)

Quote:

chrismcq said:








And on this weeks episode of murder she wrote....


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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: cronicr]
    #22160000 - 08/29/15 10:09 AM (8 years, 5 months ago)

:lol: yeah, It pains me to destroy my tub after the work i've put in to it but grows can only improve with agar :thumbup:



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InvisibleFantastic Mr. Fox
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: cronicr]
    #22160390 - 08/29/15 11:42 AM (8 years, 5 months ago)

Quote:

cronicr said:
Quote:

chrismcq said:








And on this weeks episode of murder she wrote....




:lmafo:


--------------------
Giving is all we have, for we're just grateful to be alive

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* Which strains pin and fruit EASIEST?
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Fd3000 9,572 21 08/30/02 06:22 AM
by blacktarropium

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