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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS
    #22061749 - 08/08/15 07:54 AM (8 years, 5 months ago)

Hi Guys - My second grow is under way --
All are MS, Pictures are not great but i'll be sure to update with better pics soon.

1 x Z-strain Mini Tub (SGFC)
1 x Z-strain Monotub
1 X Peuto Rican Monotub

My Monotub has a large crack down the middle because of the flex in the tub but from research I hope this wont be a problem.
Knotting has appeared overnight, both have been in fruiting conditions for 3 days. These are Z-strain -



My Largest Monotub is Peuto Rican and i'll be putting this in to fruiting conditions in a few days.
( 4 Cakes Inoculated with Agar mycelium Syringe - 1 print/ 1 clone)



My next Grow I hope to use An agar Isolate or at very least a clean agar wedge transfer.
Im having a few problems with white specks :thumbdown: (bacteria/contam)?
Its LME + Agar Agar Mix and done inside a SAB sealed with Parafilm inside a plastic baggie


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Invisibleblindingleaf
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22061885 - 08/08/15 08:55 AM (8 years, 5 months ago)

try to pick up ur tubs more by the bottom/base, instead of the handles/top, that usually helps me avoid cracking when moving them.

u can easily transfer from that contam, looks more like bacteria than mold.  check ur other plates to make sure they don't have spots before transferring, if they do, up ur PC time for agar by ten minutes.  if they don't, just move a little slower when working with plates in SAB.

good luck mang!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22062165 - 08/08/15 10:29 AM (8 years, 5 months ago)

Thank's for the advice!
I will lift my tubs from the base in the future and up my PC Time by 10 Mins :thumbup:
A few of my plates have the same bacteria / white spots so the Pc'ing may be the problem :stoned:

(update) First Pin! :grin:



Edited by chrismcq (08/08/15 05:45 PM)


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Offlinechrismcq
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Registered: 04/21/15
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22066662 - 08/09/15 11:03 AM (8 years, 5 months ago)

Pic Update -


Mini Tub -


The small tub is doing better than expected, The colonisation time was pretty slow and its still looking very brown but it is knotting nicely.
The mix is coir and vermiculite with rye grain, I think i overdid the substrate to spawn mix :ooo: or maybe its the unreliable MS Genetics.

80 New Petri dishes on the way :dancer:
The Clone i took was from a second flush mushroom, Does anyone have experience or knowledge on doing this ? It was large but i've recently tried these mushrooms (same tub) And the potency was disappointing, Im thinking of throwing the clone agar work and stick with the print? I hope to get a good specimen from my current grows and will take a clone from them im the hope to find some potent ones.. I Have took my Peuto rican tub out from colonisation as i've seen small pins showing up. I would love to try PE/APE But its very difficult for me to get hold of here in the UK.


Edited by chrismcq (08/10/15 02:32 PM)


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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22072927 - 08/10/15 06:14 PM (8 years, 5 months ago)

Mini tub, Pins are everywhere but the top ?  :crazy:  :rofl:

(Bottom of Tub)


(Side of Tub)


Edited by chrismcq (08/12/15 10:04 AM)


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Offlinechrismcq
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Registered: 04/21/15
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Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22080670 - 08/12/15 10:06 AM (8 years, 5 months ago)

Z Strain -




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Offlinechrismcq
Male


Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22094144 - 08/15/15 09:27 AM (8 years, 5 months ago)

Picked some Z-Strain today :rockon: First Flush MS Grow





Took a Clone of 2 of these - Nice little cluster :thumbup:

Mini tub update -



I've got almost 100% success rate with agar after increasing PC time by 15 mins ( Was 30 - now 45 ) So thanks for the great advice blindingleaf! :bow2: :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


Edited by chrismcq (08/15/15 02:05 PM)


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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22101820 - 08/17/15 08:13 AM (8 years, 5 months ago)

Sorry for the bad quality photos -
I'm unsure of what dish to use for my next grow.. can someone please give me a little advice? is this a isolate ?
These are prints from B+ Sectored around 5 times..  Started with a print



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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22102060 - 08/17/15 09:53 AM (8 years, 5 months ago)

I love PR.

:love:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22103003 - 08/17/15 02:28 PM (8 years, 5 months ago)

Me too :cool: Its a great looking shroom and i had good results with them with Cakes.

What do you think of the malabar strain? I Have a syringe and would like to try grow some but am worried it will contaminate due to it being a syringe.. My previous attempt was a malabar cake but turned out contaminated with mycogone ( Likely present in the syringe ) Agar will solve this but Syringe>Agar is very bad, I would love a clone or print makes things much easier..

Is there any way of growing the mycelium on agar without using the sringe solution directly on agar ?
Eg - can inoculate grain or something else and transfer that to agar?

I Got lots of petris and strains currently growing on agar and im going to have to stick with one or two strains for future grows rather than having a ton of overgrown petri's.. Maybe i should invest in a mini fridge :tongue2:
My grow area is small and i cant use any more :crazy: I would love to have more but takes up a lot of space.

I will update photos of my PR Shrooms soon. At the moment its looking clustered and has an uneven pin set but its given me a good clone (hopefully) for my next grow :thumbup:


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Invisibleblindingleaf
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103039 - 08/17/15 02:42 PM (8 years, 5 months ago)

be careful with mycogone, that is a white mold, hides easily on top of mycelium.

u can do grain>agar.  i think people do that when they can't get it to germinate on plates from spore solution.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinechrismcq
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Registered: 04/21/15
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Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22103222 - 08/17/15 03:31 PM (8 years, 5 months ago)

Thanks! :smile:
Yeah mycogone is a sneaky bastard :lurker:

I'll try MS > Agar once more and hope for better success than last time ( :syringe: Took 20 days to germinate - by that time the plates contaminated ) If this fails grains it is :thumbup:


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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103278 - 08/17/15 03:48 PM (8 years, 5 months ago)

Malabar has a tendency to be contaminated out of the syringe so I'd put it on agar. I have a needle I just can't seem to get clean :shrug:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Invisibleblindingleaf
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103285 - 08/17/15 03:49 PM (8 years, 5 months ago)

try squirting some of ur spore solution onto the plate, maybe 2- drops, then pouring ur warm-hottish liquid agar in the plate.  it will kinda heat up the spores u want (cube), hopefully softening them.  cubes can grow thru 3d matrix easy, but mold has a harder time with that.  usually u will see germination around the edges of the plate.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22103289 - 08/17/15 03:50 PM (8 years, 5 months ago)

:rockon:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinechrismcq
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Registered: 04/21/15
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22103506 - 08/17/15 04:44 PM (8 years, 5 months ago)

That's some great advice Thanks!!  :takingnotes: I will try that next time i mix up some agar.

What do you guys recommend when using MEA Agar ?
I Currently use 3 gram LME / 5 Gram Agar Agar 260ml water. I Started off using the normal 5g / 5g / 250ml but felt the agar was a little too solid when set and researching has lead me to believe that less LME / Nutrients = Encourages Rhiz Growth so cut down on them.


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Invisibleblindingleaf
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22103621 - 08/17/15 05:16 PM (8 years, 5 months ago)

i like more solid agar, so i use 11g or so per 500ml.
but it sucks for spore germination, so i do the spores first>pour.

i never cut nutrients anymore.  yes, it makes things rhizo.  but other than that, not sure what good it ever did me.  but, there are many other growers on here who do it, so theres no wrong way really.  goal should be clean mycelium in any and all cases.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinetaGyo
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Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: blindingleaf]
    #22103682 - 08/17/15 05:31 PM (8 years, 5 months ago)

:whathesaid:

The most annoying thing in the world is cutting nutrients and getting super rhizo myc and then putting it in an LI and throwing it in grains and it immediately turns tomentose as fuck and colonizes slowly. Neither bother me anymore though, it's all about that pinset.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: taGyo]
    #22106978 - 08/18/15 02:34 PM (8 years, 5 months ago)

Okay thank you guys, I will keep experimenting and do what works best for me :thumbup:
I Will update with some PR Photos tomorrow and will have to take another clone from them.. My first 2 contaminated with bacteria due to being too small, Turned out very difficult to rip the small shroom / pin in half and cut out a small section to transfer.. I can take a larger specimin tomorrow for cloning :smile:


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Offlinechrismcq
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Registered: 04/21/15
Posts: 113
Last seen: 7 years, 9 months
Re: Second Grow - Cracked Monotub - Z Strain + Peuto Rican MS [Re: chrismcq]
    #22110938 - 08/19/15 11:29 AM (8 years, 5 months ago)

PR First Small Shrooms :dancer:




Edited by chrismcq (08/19/15 02:23 PM)


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