Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
DAMNED AGAR
    #22061347 - 08/08/15 01:29 AM (8 years, 5 months ago)

Although the title is a little dramatic, ive been having some frustrating problems with agar lately.  On my most recent batch, I started a MS syringe(Treasure Coast) on agar.  fast forward 2 transfers and I have maybe 5/20 contamed with the same orange bacteria from the previous transfers, those plates were tossed immediately.  But whats stumping me is this stuff, kinda looks like mycelium(not really though) and kinda like bacteria, so I don't really know what it is.  It was on maybe 8 or 9 plates, and I have separated a few that had the smallest amount of suspected contam and tossed the rest. 

(you can clearly see it at the ends of the mycelium towards the edges of the plates.  On the pic with the tape, its under the tape towards the edge.)

I know you vets mustve seen this before, and could hopefully share what caused it, whether it be your procedure or something.  My technique is getting better, not perfect(had 1 contam out of 30 plates using a 'hood), but I do have a couple perfect plates from the same batch.  On top of that, I have some Boner Clones which I just transferred to new plates, so ill post what those look like and if they have the same contam in a few days.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Edited by firstTIMER420 (08/08/15 07:00 PM)


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: DAMNED AGAR [Re: firstTIMER420]
    #22061358 - 08/08/15 01:39 AM (8 years, 5 months ago)

It looks like a mold contamination. You can check this thread out i made recently on them lol http://www.shroomery.org/forums/showflat.php/Number/22020260


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: DAMNED AGAR [Re: firstTIMER420]
    #22061372 - 08/08/15 01:50 AM (8 years, 5 months ago)

Clip your damn fingernails.

Then, and only then, refine your SAB technique.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: DAMNED AGAR [Re: Buck513]
    #22061423 - 08/08/15 02:33 AM (8 years, 5 months ago)

sorry to disappoint buck, but those are my girls hands. that reply almost didn't even deserve a response.

and mad, that's what I was thinking because it didn't look bacterial to me, I just want to get some cleaner plates because its starting to piss me off.  I just switched SAB's so hopefully itll get better, like I said im going to post pics from the new plates in a day or so, but they seem to be doing good so far.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: DAMNED AGAR [Re: firstTIMER420]
    #22061539 - 08/08/15 04:55 AM (8 years, 5 months ago)

i cant see much on your plates, but how about not incriminating another member in your OP?


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: DAMNED AGAR [Re: Buck513]
    #22061547 - 08/08/15 05:11 AM (8 years, 5 months ago)

Quote:

Buck513 said:
Clip your damn fingernails.

Then, and only then, refine your SAB technique.




:rofl:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Offlinepurplecap
candyflipper


Registered: 06/10/15
Posts: 150
Last seen: 1 year, 5 months
Re: DAMNED AGAR [Re: blindingleaf]
    #22062127 - 08/08/15 10:12 AM (8 years, 5 months ago)

What do you wrap them in ? Orange stuff for me mostly appears when I'm not careful. Are you sure your plates are poured right ? Have a few empty ones left aside to see if they contam on their own ?


Extras: Filter Print Post Top
InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: DAMNED AGAR [Re: firstTIMER420]
    #22062197 - 08/08/15 10:41 AM (8 years, 5 months ago)

Quote:

firstTIMER420 said:
that reply almost didn't even deserve a response.



99.8% of your posts don't deserve a response. You refuse to listen to the advice people give you time, and time again.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: DAMNED AGAR [Re: Buck513]
    #22064082 - 08/08/15 06:59 PM (8 years, 5 months ago)

I would love to see some examples of this buck, because it seems you keep saying that but in reality its not true.

and I either wrap them in saran wrap or put them in a bag.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: DAMNED AGAR [Re: firstTIMER420] * 1
    #22064767 - 08/08/15 10:12 PM (8 years, 5 months ago)

[Quote]firstTIMER420 said:
I would love to see some examples of this buck, because it seems you keep saying that but in reality its not true.

and I either wrap them in saran wrap or put them in a bag.



:facepalm:Only everyone on shroomery feels this way.

You have 3 pages of posts asking questions, 90% are in mush cult.
Wanna see some examples? Click this link and pick one:shrug:
http://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&namebox=354530&limit=25&sort=d&way=d&page=0


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: DAMNED AGAR [Re: Buck513]
    #22065220 - 08/08/15 11:58 PM (8 years, 5 months ago)

I ask questions that are specific to my situation and experiences asking for YOUR experiences and solutions to similar situations.  Most of which cannot be answered by me just looking up old outdated info.  I get it man, picking on the new guy makes you feel good about yourself, good for you.  But honestly, I could care less. Dude was right, someone doesn't like someone so here the bandwagon comes. Think for yourself?


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Extras: Filter Print Post Top
InvisibleSteveRogers
gandy dancer
 User Gallery

Registered: 10/24/06
Posts: 3,450
Re: DAMNED AGAR [Re: firstTIMER420] * 2
    #22065241 - 08/09/15 12:02 AM (8 years, 5 months ago)

Quote:

firstTIMER420 said:
Think for yourself?



Take your own advice here, partner. You have been abusing this website's resources, seemingly without learning a thing.


--------------------
"General, I am loyal to nothing......except The Dream"


Edited by SteveRogers (08/09/15 12:15 AM)


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: DAMNED AGAR [Re: SteveRogers]
    #22065284 - 08/09/15 12:12 AM (8 years, 5 months ago)

i can't see much from those pics


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: DAMNED AGAR [Re: cronicr]
    #22065328 - 08/09/15 12:22 AM (8 years, 5 months ago)

the second pic has the best view of it.  The contam itself is almost clear but with a yellow tint, white center in it.

and steve, I do not repost the same questions over and over as implied, so your not really making a point.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Edited by firstTIMER420 (08/09/15 12:25 AM)


Extras: Filter Print Post Top
OfflineAl Bundy
No Ma'am
Male


Registered: 05/01/15
Posts: 178
Loc: Chicago
Last seen: 2 years, 9 months
Re: DAMNED AGAR [Re: SteveRogers]
    #22065405 - 08/09/15 12:41 AM (8 years, 5 months ago)

Quote:

SteveRogers said:
Quote:

firstTIMER420 said:
Think for yourself?



Take your own advice here, partner. You have been abusing this website's resources, seemingly without learning a thing.



This.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,156 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,135 8 06/19/20 09:41 PM
by gorilliam
* Will spores germinate in agar? MycoJunkie 14,999 12 11/26/20 11:15 PM
by KKVguitarist
* Simplified agar for a newbie, please!
( 1 2 all )
naturalistic123 629 21 05/02/18 11:33 AM
by iux
* Agar question for the experts Nunyabiz 157 4 04/21/18 01:04 PM
by JHOVA
* What to do with agar? HerbanShaman 1,910 15 04/21/05 02:27 AM
by MushroomFriend
* Antibac Agar, DIY! superduper 4,846 13 12/04/02 02:20 PM
by superduper

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
694 topic views. 41 members, 254 guests and 61 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.011 seconds on 14 queries.