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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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DAMNED AGAR
#22061347 - 08/08/15 01:29 AM (8 years, 5 months ago) |
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Although the title is a little dramatic, ive been having some frustrating problems with agar lately. On my most recent batch, I started a MS syringe(Treasure Coast) on agar. fast forward 2 transfers and I have maybe 5/20 contamed with the same orange bacteria from the previous transfers, those plates were tossed immediately. But whats stumping me is this stuff, kinda looks like mycelium(not really though) and kinda like bacteria, so I don't really know what it is. It was on maybe 8 or 9 plates, and I have separated a few that had the smallest amount of suspected contam and tossed the rest.
(you can clearly see it at the ends of the mycelium towards the edges of the plates. On the pic with the tape, its under the tape towards the edge.)
I know you vets mustve seen this before, and could hopefully share what caused it, whether it be your procedure or something. My technique is getting better, not perfect(had 1 contam out of 30 plates using a 'hood), but I do have a couple perfect plates from the same batch. On top of that, I have some Boner Clones which I just transferred to new plates, so ill post what those look like and if they have the same contam in a few days.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
Edited by firstTIMER420 (08/08/15 07:00 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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It looks like a mold contamination. You can check this thread out i made recently on them lol http://www.shroomery.org/forums/showflat.php/Number/22020260
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Buck513
Registered: 04/17/14
Posts: 5,682
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Clip your damn fingernails.
Then, and only then, refine your SAB technique.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: DAMNED AGAR [Re: Buck513]
#22061423 - 08/08/15 02:33 AM (8 years, 5 months ago) |
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sorry to disappoint buck, but those are my girls hands. that reply almost didn't even deserve a response.
and mad, that's what I was thinking because it didn't look bacterial to me, I just want to get some cleaner plates because its starting to piss me off. I just switched SAB's so hopefully itll get better, like I said im going to post pics from the new plates in a day or so, but they seem to be doing good so far.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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i cant see much on your plates, but how about not incriminating another member in your OP?
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: DAMNED AGAR [Re: Buck513]
#22061547 - 08/08/15 05:11 AM (8 years, 5 months ago) |
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Quote:
Buck513 said: Clip your damn fingernails.
Then, and only then, refine your SAB technique.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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purplecap
candyflipper


Registered: 06/10/15
Posts: 150
Last seen: 1 year, 5 months
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What do you wrap them in ? Orange stuff for me mostly appears when I'm not careful. Are you sure your plates are poured right ? Have a few empty ones left aside to see if they contam on their own ?
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Buck513
Registered: 04/17/14
Posts: 5,682
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Quote:
firstTIMER420 said: that reply almost didn't even deserve a response.
99.8% of your posts don't deserve a response. You refuse to listen to the advice people give you time, and time again.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: DAMNED AGAR [Re: Buck513]
#22064082 - 08/08/15 06:59 PM (8 years, 5 months ago) |
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I would love to see some examples of this buck, because it seems you keep saying that but in reality its not true.
and I either wrap them in saran wrap or put them in a bag.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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Buck513
Registered: 04/17/14
Posts: 5,682
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[Quote]firstTIMER420 said: I would love to see some examples of this buck, because it seems you keep saying that but in reality its not true.
and I either wrap them in saran wrap or put them in a bag.
Only everyone on shroomery feels this way.
You have 3 pages of posts asking questions, 90% are in mush cult. Wanna see some examples? Click this link and pick one http://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&namebox=354530&limit=25&sort=d&way=d&page=0
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: DAMNED AGAR [Re: Buck513]
#22065220 - 08/08/15 11:58 PM (8 years, 5 months ago) |
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I ask questions that are specific to my situation and experiences asking for YOUR experiences and solutions to similar situations. Most of which cannot be answered by me just looking up old outdated info. I get it man, picking on the new guy makes you feel good about yourself, good for you. But honestly, I could care less. Dude was right, someone doesn't like someone so here the bandwagon comes. Think for yourself?
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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SteveRogers
gandy dancer


Registered: 10/24/06
Posts: 3,450
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Quote:
firstTIMER420 said: Think for yourself?
Take your own advice here, partner. You have been abusing this website's resources, seemingly without learning a thing.
-------------------- "General, I am loyal to nothing......except The Dream"
Edited by SteveRogers (08/09/15 12:15 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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i can't see much from those pics
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: DAMNED AGAR [Re: cronicr]
#22065328 - 08/09/15 12:22 AM (8 years, 5 months ago) |
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the second pic has the best view of it. The contam itself is almost clear but with a yellow tint, white center in it.
and steve, I do not repost the same questions over and over as implied, so your not really making a point.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
Edited by firstTIMER420 (08/09/15 12:25 AM)
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Al Bundy
No Ma'am



Registered: 05/01/15
Posts: 178
Loc: Chicago
Last seen: 2 years, 9 months
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Quote:
SteveRogers said:
Quote:
firstTIMER420 said: Think for yourself?
Take your own advice here, partner. You have been abusing this website's resources, seemingly without learning a thing.
This.
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