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Offlinedonkeycong
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Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Should I toss the rest of the jars?!
    #22055832 - 08/06/15 09:56 PM (8 years, 5 months ago)

I inoculated 10 jars with a spore syringe from a sponsor. All the jars are showing pretty good growth, but as i was looking at the bottom of one and I noticed a green spot, OH NO! Pretty sure the syringe was already contaminated as I have great sterile technique and haven't had any contams in months. What would you do? toss the rest of the jars and start fresh, or let them grow and see what happens? Thanks guys!


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OfflineFreeWorldOrder
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Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA Flag
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Re: Should I toss the rest of the jars?! [Re: donkeycong]
    #22055875 - 08/06/15 10:10 PM (8 years, 5 months ago)

If one is showing green definitely get rid of that one. But I would watch the rest and see what they do.

Did you flame needle between each jar inoculation?

Wipe lids with 70% ISO?

Use a SAB?

Sterilize properly?

You may have just picked up a contam between inoculations.

It's always a chance something can land on a jar lid ya know... hope the rest turn out okay for you.


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InvisibleAureus
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Registered: 07/04/15
Posts: 478
Re: Should I toss the rest of the jars?! [Re: donkeycong]
    #22055898 - 08/06/15 10:15 PM (8 years, 5 months ago)

It's not the syringe, if the source of contamination was your syringe all the jars you inoculated with it would be green.

Green mold spores germinate first than mushroom spores, if the other jars are showing good growth, you don't need to worry.


Edited by Aureus (08/06/15 10:23 PM)


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Should I toss the rest of the jars?! [Re: Aureus]
    #22055912 - 08/06/15 10:20 PM (8 years, 5 months ago)

Toss the one with the green spot, its most likely trich, and let the other jars grow more before making any final decisions. And post some pics!!


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Offlinedonkeycong
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Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Re: Should I toss the rest of the jars?! [Re: donkeycong]
    #22055948 - 08/06/15 10:33 PM (8 years, 5 months ago)

Yea after thinking about it I'm pretty sure the needle must've picked up something funky while inoculating that one. I never wipe lids with iso, although i always use a small sponge soaked with iso on the top of inoculation point. I probably forgot to re-saturate the sponge with that one jar, as all the others look like this and have clean growth



I should've took a pic of the contaminated jar, but its in compost already


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InvisibleMad Season
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Registered: 09/16/12
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Loc: Canada Flag
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Re: Should I toss the rest of the jars?! [Re: Aureus]
    #22055957 - 08/06/15 10:37 PM (8 years, 5 months ago)

Quote:

Aureus said:
It's not the syringe, if the source of contamination was your syringe all the jars you inoculated with it would be green.

Green mold spores germinate first than mushroom spores, if the other jars are showing good growth, you don't need to worry.



Both statements are assumptions that can be shown as false. When you look under the scope or inoculate with agar.. you can and almost always do get a different type of contam spore in each drop of spore solution. In the same syringe of spores I've seen aspergillus, trichoderma, some weird white thing, an extremely thin mold that spread just as fast as cobweb, a bunch of different types of bacteria, and of course the couple of plates that are completely clean :lol:

pf tek is very forgiving. It's harder for contaminants to germinate on it. Grains aren't as forgiving. And agar is basically the least forgiving. Which is why it's the best at working on your sterile techniques.

I've also seen mycelium grow first, and then the green showed up a couple days later. Which I didn't mind because it's easy to clean up green mold. It's aggressive bacteria that makes me wanna bitch, or the Hidden contams which if you look in the white molds section shows a good example of a green mold that hasn't shown itself yet


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Edited by Mad Season (08/06/15 10:42 PM)


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Should I toss the rest of the jars?! [Re: donkeycong]
    #22055977 - 08/06/15 10:43 PM (8 years, 5 months ago)

Quote:

donkeycong said:
Yea after thinking about it I'm pretty sure the needle must've picked up something funky while inoculating that one. I never wipe lids with iso, although i always use a small sponge soaked with iso on the top of inoculation point. I probably forgot to re-saturate the sponge with that one jar, as all the others look like this and have clean growth



I should've took a pic of the contaminated jar, but its in compost already



That hennessey bottle tho!!! Good shit brotha, that's my favorite drank!! :awesomenod:

Back on topic, When inoculating jars in your SAB, just flame your needle until red hot, then inject thru the SHIP (no need to inject thru the alcohol pad, just wipe it down once), better yet, skip the SHIP for your next round of jars. You only really need a GE hole. Then crack the lid in your SAB when your ready and squirt your solution in OR if you have it grown on agar, cut a wedge from that, and drop it in!! That's the beauty of working with agar, you can see clean growth, MS is a unpredictable and unreliable most of the time.

Anyway, no need to worry, that jar you have there is showing good growth, just keep an eye on it, and shake it once it's about 30% colonized to redistribute the unexposed grains and wait until 100%! Then spawn to bulk! :goodluck:


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