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Jsneeb
Drifter


Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
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Someone who grew Pan Cyans. HOW? Not pinning.
#22055399 - 08/06/15 08:17 PM (8 years, 5 months ago) |
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Ok, I've been struggling like damn to get some Cyans and really have no idea what my problem is.
Here is the procedure and current stages.
May 27th, 2 Spore prints received.
Spokes put on agar to germinate and "clean up".
after 2 weeks first transfer made.
After that transfer 2 more were made, 4 days apart.
3rd selection made into LI, Inoculated 3 jars of WBS. Good growth.
Jars spawned to straw, Contamed within 3 days, Confirmed bacteria, Tossed
Agar --> grain, good growth. 2 quart jars and one 1/2 pint widemouth
Jars spawned to maure/coir with coir/verm casing.
Monotub misted lightly for 1 week then no misting and upped the airflow.
Temps kept between 75-80 indoors.
Continued for 2 weeks, no pins, substrate got very dry.
Resumed misting,
This mini-monotub sat for over 5 weeks. Nothing. Contamed, Trich, Tossed.
1/2 pint wide mouth lid loosened and allowed to pin invitro.
Sat for 5 weeks, nothing,
Dried out. Tossed.
Meanwhile I've pulled 4 flushes of my first monotub of cubes, 2 of my second, and about 4 more from my outdoor patch. So I know how to grow cubes.
So, I'm thinking my strain just isn't capable of pinning? WTF? Is that possible? Since I started from a print, is that all one single genetics?
I still have agar transfers from that batch in the fridge ( on its 5th or 6th transfer now ) But I'm also going to take the other print and start from scratch.
Any ideas on what I'm doing wrong?
-------------------- Read my trip report Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter? http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Someone who grew Pan Cyans. HOW? Not pinning. [Re: Jsneeb]
#22055462 - 08/06/15 08:34 PM (8 years, 5 months ago) |
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How deep is the sub? Try peat and verm to case
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Sagescruffy
CH



Registered: 10/30/09
Posts: 2,011
Loc: PNW
Last seen: 6 months, 12 days
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Re: Someone who grew Pan Cyans. HOW? Not pinning. [Re: cronicr]
#22055638 - 08/06/15 09:09 PM (8 years, 5 months ago) |
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You could do macmerdins simple pan cakes or whatever and probably see fruits. As for why they aren't pinning, there isn't enough information there. It's possible they're over heating, they are known to do that. Kinda makes sense considering your approach to them. Had you used aluminum trays and fruited in a sgfc you might have seen some fruits. SGFC> monotub for the exotics. Not to say monotubs can't produce good fruits, but there's no doubt that a sgfc has better conditions for pans than a monotub.
-------------------- Love.  
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Jsneeb
Drifter


Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
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Re: Someone who grew Pan Cyans. HOW? Not pinning. [Re: Sagescruffy]
#22072217 - 08/10/15 03:23 PM (8 years, 5 months ago) |
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Ok.
I'll give the SGFC a shot in the coming months.
-------------------- Read my trip report Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter? http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/
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