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PoIIo
Stranger
Registered: 08/05/15
Posts: 83
Last seen: 8 years, 1 month
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Possible contam with request for advice
#22054938 - 08/06/15 06:31 PM (8 years, 5 months ago) |
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inoculated 10 jars from a sponsor with a MS from another sponsor on 7/16. Lost two jars quickly to green mold and another stalled completely due to bacteria (sweet smell). The following pictures are the last 9 jars which seem to be going slower than I expected.

Only one jar seems to be close to or at 100%. The others have large patches of uncolonized grain. I have a PC on the way should I just gtg the 1 good jar? Or should I just mini tub the 1 or 2 good jars? I used a SAB with as good as sterile tek as I could but still managed to mess up a good amount of jars. Was hoping to do Damions tek.
All jars with growth were shaken on 7/30 and 7/31.
Edited by PoIIo (08/06/15 07:56 PM)
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Re: Possible contam with request for advice [Re: PoIIo]
#22055829 - 08/06/15 09:55 PM (8 years, 5 months ago) |
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Quote:
PoIIo said: Only one jar seems to be close to or at 100%. The others have large patches of uncolonized grain. I have a PC on the way should I just gtg the 1 good jar? Or should I just mini tub the 1 or 2 good jars?
ok. I don't want to bad mouth a sponsor and certainly not implicate them in anything so I am assuming you inoculated those jars with Oyster spores. I know exactly where you got those jars and that sponsor recently changed from using syringe filters to using those SFDs that you have on your. Air exchange was a huge issue before, as well as the grains being too wet. At this point I would wait for your PC to come and prepare your own jars. You can try and spawn the ones that make it eventually to a bulk sub, but there were a lot of issues with this vendors jars stalling flat out in the past.
Quote:
PoIIo said: All jars with growth were shaken on 7/30 and 7/31.
Did you shake them twice or just on different days because the jars were ready at different times?
-------------------- "General, I am loyal to nothing......except The Dream"
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PoIIo
Stranger
Registered: 08/05/15
Posts: 83
Last seen: 8 years, 1 month
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Re: Possible contam with request for advice [Re: SteveRogers]
#22056657 - 08/07/15 04:32 AM (8 years, 5 months ago) |
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They were ready at different times. Funny you mention the air exchange. I just dumped the obviously bad jars and noticed how small the air exchange hole was compared to what I see on the forum. I ended up loosening the lids a quarter turn.
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Re: Possible contam with request for advice [Re: PoIIo]
#22056848 - 08/07/15 06:52 AM (8 years, 5 months ago) |
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Yes, as I said that vendor has had issues with that. I cannot figure out why they make them in that way. The guy who runs it is very smart and a really nice guy. He has sent me almost double my order every time I get raw materials from him.
When I saw the syringe filters on his grain masters being sold, I asked him if there had been issues with stalling. He confided in me that in fact it had been a major problem and was switching to the SFD's that you have. He sent me some of the lids to try on my own jars and every one stalled.
You should still continue to wait though. For a one quart jar from multispore, you are still within a reasonable time frame as inside of 30 days you have already shaken and seen significant recovery.
Continue to wait, but if it comes down to it, I have had success placing jars on their sides at a 45 degree and or enough that the grains don't spill onto the bottom of the lid. I assume it affected the GE, but couldn't say exactly why it helped them finish colonizing. RogerRabbit has mentioned doing this as well.
Good luck. Just be patient. MS is a crapshoot.
-------------------- "General, I am loyal to nothing......except The Dream"
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PoIIo
Stranger
Registered: 08/05/15
Posts: 83
Last seen: 8 years, 1 month
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Re: Possible contam with request for advice [Re: SteveRogers]
#22056875 - 08/07/15 07:14 AM (8 years, 5 months ago) |
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Incredible response. I appreciate it. I think I'm also going find a good lid tek and make my own with polyfill etc. I don't want to reuse these jars if they can't breathe.
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Re: Possible contam with request for advice [Re: PoIIo]
#22056886 - 08/07/15 07:20 AM (8 years, 5 months ago) |
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Citric's Lid Tek is a good one. He uses Tyvek, but I would recommend SFD's. The concept is the same though either way.
You can get away with poly if you stuff it correctly, just more BS than I am willing to deal with.
It can be very frustrating to get something from a trusted source that does not perform well. Do not lose heart, you are in the right place to get where you want to go.
Good luck to you.
-------------------- "General, I am loyal to nothing......except The Dream"
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