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Offlinekierbob
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Registered: 06/18/15
Posts: 79
Last seen: 5 years, 5 months
First time agar (Pleurotus osteatus)
    #22054797 - 08/06/15 06:06 PM (8 years, 5 months ago)

Hey guys, please bare with me. Moved this post from "Contamination" as it's a ghost town over there.

I've been doing a ton of reading, both on the forum and a few of Stamets books.
Still I am left with an abundance of questions as new things arise every day.

Anyway, dipping into agar trying to clean up some Pleurotus osteatus myc from some commercial spawn.

The plates are malt extract agar and are 5 days old.

Clear radial growth with airborne fluff.

Myc? Contaminate? Weak growth?

My main question is if this is suitable for transfer, where and when should I begin?

Thanks guys!



*Edit*
Quote:

Mycelial Characteristics: Whitish, longitudinally radical, soon becoming cottony, and in age forming a thick, tenacious mycelial mat. Aged mycelium often secretes yellowish to orangish droplets of a metabolite which is a toxin to nematodes. This metabolite deserves greater study.




This sounds like what I'm looking at and fits with Stamets description of longitude and cotton mycelium characteristics. It's just so clear and light, almost like a film or residue on the agar.


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OfflineTweakz
Professional contam grower


Registered: 07/09/15
Posts: 154
Last seen: 1 month, 27 days
Re: First time agar (Pleurotus osteatus) [Re: kierbob]
    #22056439 - 08/07/15 01:59 AM (8 years, 5 months ago)

looks like cobweb mould.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: First time agar (Pleurotus osteatus) (moved) [Re: kierbob]
    #22056449 - 08/07/15 02:07 AM (8 years, 5 months ago)

This thread was moved from Mushroom Cultivation.

Reason:
:super:


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: First time agar (Pleurotus osteatus) (moved) [Re: cronicr]
    #22056490 - 08/07/15 02:27 AM (8 years, 5 months ago)

Looks like cobweb but the flat surface growth could be weak myc but doubtful and if it was the cobweb spores are all ready released in there so saving becomes hard. You want to transfer at the first sings of contamination.


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Offlinekierbob
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Registered: 06/18/15
Posts: 79
Last seen: 5 years, 5 months
Re: First time agar (Pleurotus osteatus) (moved) [Re: vatman]
    #22056880 - 08/07/15 07:17 AM (8 years, 5 months ago)

Cheers guys! Definitely looks like cobweb has completely taken over.


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Offlinebryanbzl
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Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: First time agar (Pleurotus osteatus) (moved) [Re: vatman]
    #22056895 - 08/07/15 07:24 AM (8 years, 5 months ago)

Hey kierbob,

I have had blue oyster and lions mane that look exactly like that. At thirst it starts like that flat radial growth and then thickens up and goes aerial. Keep an eye on it. IME If that was cobweb that whole plate would be consumed incredibly fast. 5 days old tells me you should be okay buddy. If it grows at a nice pace daily then I would cut a wedge to agar (minimum 3 plates).

In my experience so far with agar I have had very little mould contamination but my contaminants tend to be bacterial. look for a discoloured surface - typically a whitish yellow discolouration and the smell will have changed. Best I could describe it is a fruity(fermented) or cheesy smell.

Hope this helps you. Any q's shoot me a PM.


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Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


Edited by bryanbzl (08/07/15 07:26 AM)


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Offlinebryanbzl
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Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: First time agar (Pleurotus osteatus) (moved) [Re: bryanbzl]
    #22056907 - 08/07/15 07:31 AM (8 years, 5 months ago)

Also, I transfer wedges from the outermost and fastest growing mycelium. Basically the newest growth. Wisdom says its the youngest and most vigorous growth (best for transferring).


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlinekierbob
Stranger

Registered: 06/18/15
Posts: 79
Last seen: 5 years, 5 months
Re: First time agar (Pleurotus osteatus) (moved) [Re: bryanbzl]
    #22065590 - 08/09/15 02:13 AM (8 years, 5 months ago)

Well, I left these plates way to long as I've been busy.

I attempted a transfer today but no idea how it will go.

So now I have the first plates with the lid off for a good photo, what are we looking at? Cottony aerial mycelium producing coremia? I just read about oysters producing coremia (spore bundles) in Stamets book.

Or is this simply just some type of black mold?





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Invisiblefollowthefungi
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Registered: 08/08/15
Posts: 8
Re: First time agar (Pleurotus osteatus) (moved) [Re: kierbob]
    #22066012 - 08/09/15 07:55 AM (8 years, 5 months ago)

That looks like some nasty mold to me. Sorry, but I think it's a loss.


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Offlineforrest
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Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 6 months
Re: First time agar (Pleurotus osteatus) (moved) [Re: followthefungi]
    #22066084 - 08/09/15 08:18 AM (8 years, 5 months ago)

yeah, looks bad. but don;t you mean conidia instead of coremia?


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Offlinekierbob
Stranger

Registered: 06/18/15
Posts: 79
Last seen: 5 years, 5 months
Re: First time agar (Pleurotus osteatus) (moved) [Re: forrest]
    #22067465 - 08/09/15 02:24 PM (8 years, 5 months ago)

Stamet refers to coremia in his book keeping. It's the first I've ever head of either!

Quote:

A synnema (plural synnemata, also coremia; derivation: "Threads together") is a large, erect reproductive structure borne by some fungi, bearing compact conidiophores,[1] which fuse together to form a strand resembling a stalk of wheat, with conidia at the end or on the edges




A well, so I succeeded in growing some funky looking hairy ass mold! Yay...hah


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