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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Bacteria?
#22054582 - 08/06/15 05:13 PM (8 years, 5 months ago) |
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Hey all!
So I have ten different master jars, each inoculated with thier very own plate, which were all started from two different cultures germinated from spores
These plates, as far as I could tell were immaculate.heres one I think


Heres photos of 2 different jars, all the jars look lije this kinda solis with fluffy myc pushed up against the glass with that smooshed look. They look clean just weird
I had let the agar colonize about a third of the jar before I shook so they colonied wicked fast, the growth was super healthy before I shook.
I left town for a few days and the air conditioning went off so maybe they got a little warmer but not crazy hot, I do see some drops of metabolites and thats the main point that has me worried.
Thee grain was PC for 2.5 hours and the inoculation was very on point. I might sacrifice one to give a good smell and do shake test on others
These are going to be G2G into 4qt spawn. Would you use these masters?
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 Amateur Mycologists United AMU Q&A
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea, looks like bacteria to me. probably the AC. with that plate, and those PC times. sorry dude
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Quote:
blindingleaf said: yea, looks like bacteria to me.
This.
I've gotten many a flush off a bacterial spawn jar though. All is not lost. 
Quote:
mushpunx said: Would you use these masters?
I would not use them as masters, but as spawn for their own mini monos/trays ect.
-------------------- "General, I am loyal to nothing......except The Dream"
Edited by SteveRogers (08/06/15 05:59 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Ewwwe what a bummer!
Yea I shook them right before I left town for 5 days, and the AC quit.. guess it got real hot here too. Didnt think it woulda got that hot inside but who knows those were stacked in my flow hood room which stays all shut up
I had few tubs colonizing in there just spawned before I left, they look alright though 
Heres a PS Mexicana jar that was in there too it looks happy fortunately Time for a shake
Thanks guys
Edited by mushpunx (08/06/15 05:58 PM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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i just moved, and got a new window unit that has an "auto setting" for energy saving, and goes on and off depending at what temp u set it at. was only $20 i think than the next one down. pretty sweet if u can find one at end of summer on the cheap for next year maybe
that happened to me this winter with my heater, got way too hot when i was away.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Quote:
blindingleaf said: i just moved, and got a new window unit that has an "auto setting" for energy saving, and goes on and off depending at what temp u set it at. was only $20 i think than the next one down. pretty sweet if u can find one at end of summer on the cheap for next year maybe
that happened to me this winter with my heater, got way too hot when i was away.
Oh thats awesome I was looking for one of those for an attic before, I almost bought a power strip that would turn on/ off according to a thermostat.
Im betting it was the temp, I could see a couple jars going bacterial but all ten from ten different plates? Id like to think Im not *that* sloppy
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (08/06/15 06:31 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Quote:
SteveRogers said:
Quote:
blindingleaf said: yea, looks like bacteria to me.
This.
I've gotten many a flush off a bacterial spawn jar though. All is not lost. 
Quote:
mushpunx said: Would you use these masters?
I would not use them as masters, but as spawn for their own mini monos/trays ect.
Yea I think thats what Im gunna do, theyre all MS so I guess Ill just spawn 2 tubs with them.
I popped one open, the grains felt a little wet/soft but didnt smell off.. but I cant really tell for sure I have the chest cold of the decadr right now 
This is my first PE6 grow so itd be nice to see something, maybe grab a clone I was pretty excited to give these a shot!
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (08/06/15 06:30 PM)
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