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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: germination problem [Re: bodhisatta]
    #22055443 - 08/06/15 08:29 PM (8 years, 5 months ago)

Quote:

bodhisatta said:
doesn't matter it's the same agar. I know pasty says 45 but 15 would do it too. it doesn't matter you don't want your agar bottle on the bottom of the PC either.

I've never sterilized my home made or pre mix agar longer than 20m. and also never had a problem with the agar itself,

your PC takes longer to get to pressure when full, it doesn't get to 15 until things are up to temp on the inside, it's a property of the latent heat transfer of steam, it condenses on the cooler jars and looses a insane amount of heat energy until the jars heat up it won't get to 15psi.
you start the timer at 15psi.

regardless of how much agar is in your PC it will still take 15m in a 500ml jar or several small pasty plates.



Thanks for the info bod, I noticed other cultivators complaining of too much condensation in their plates, agar being too stiff, etc. It is probably because PC times are too long.

And also, when I made my agar substitute tek (brf and water), I PC'd for 15 min at 15 PC and all my brf paste jars came out good with only one contaminant out of 6 jars (probably due to lack of proper sterile technique), still a success IMO.


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InvisibleMad Season
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Registered: 09/16/12
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Re: germination problem [Re: Psilosoulful]
    #22055457 - 08/06/15 08:32 PM (8 years, 5 months ago)

Quote:

MA - Care must be taken used with malt not to exceed 10 pounds pressure for 20 minutes. Higher temperatures cause malt sugars to caramelize, preventing the agar from jelling properly.




http://www.shroomery.org/8506/Culture-Mediums


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
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OfflineTEAMSESH
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Registered: 04/08/15
Posts: 203
Last seen: 8 years, 4 months
Re: germination problem [Re: Appalachian Brony]
    #22055471 - 08/06/15 08:38 PM (8 years, 5 months ago)

didn't sterilize the plates.  only the mea in a mason jar got serialized at some 15-16 psi


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: germination problem [Re: Mad Season]
    #22055479 - 08/06/15 08:40 PM (8 years, 5 months ago)

I'm confused, are you saying that 10 psi is recommended for pasty plates then? Never made them before, so I don't know how they look like after a PC cycle. Just want to make sure I do it right the first time.


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OfflineTEAMSESH
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Registered: 04/08/15
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Re: germination problem [Re: Mad Season]
    #22055488 - 08/06/15 08:42 PM (8 years, 5 months ago)

Tanks ill give it a read :thumbup:


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OfflineTEAMSESH
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Registered: 04/08/15
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Re: germination problem [Re: TEAMSESH]
    #22055503 - 08/06/15 08:44 PM (8 years, 5 months ago)

great info hear I'm:thumbup: learning


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InvisibleMad Season
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Re: germination problem [Re: Psilosoulful]
    #22055514 - 08/06/15 08:47 PM (8 years, 5 months ago)

Quote:

Psilosoulful said:
I'm confused, are you saying that 10 psi is recommended for pasty plates then? Never made them before, so I don't know how they look like after a PC cycle. Just want to make sure I do it right the first time.



15 is fine. It's just  pulled from a lab book. It's just saying you don't want to overcook it. As bod said 15-20 minutes is fine. Going over 20 minutes, especially with malt agar would cause it to caramelize


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineTEAMSESH
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Registered: 04/08/15
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Re: germination problem [Re: bodhisatta]
    #22055566 - 08/06/15 08:57 PM (8 years, 5 months ago)

hey so the food grade agar im using is in a block forum.
weighs 10grams should I figure my own ratio to get it percept because don't know any thing that works with this agar .I've used powder in the past with 100% successes even brf agar style teks with successes. im using light malt extract to.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: germination problem [Re: Mad Season]
    #22055795 - 08/06/15 09:46 PM (8 years, 5 months ago)

Quote:

Mad Season said:
Quote:

Psilosoulful said:
I'm confused, are you saying that 10 psi is recommended for pasty plates then? Never made them before, so I don't know how they look like after a PC cycle. Just want to make sure I do it right the first time.



15 is fine. It's just  pulled from a lab book. It's just saying you don't want to overcook it. As bod said 15-20 minutes is fine. Going over 20 minutes, especially with malt agar would cause it to caramelize



Awesome, can't wait to mix up some agar!! :thumbup:


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OfflineTEAMSESH
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Registered: 04/08/15
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Re: germination problem [Re: Psilosoulful]
    #22056523 - 08/07/15 02:45 AM (8 years, 5 months ago)

If carmalization is to occur what effects would it have on germination?


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: germination problem [Re: TEAMSESH]
    #22056536 - 08/07/15 02:53 AM (8 years, 5 months ago)

I PC my plates for 90 minutes. Never had any caramalization. Also never used malt dextrose extract. I do standard potato flakes or grain water recipes. they look the same before pc as they do after. you can pour them and let them cool down to look at them before you load them. mine usually have a nice glossy sheen to them.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: germination problem [Re: Munchauzen]
    #22057392 - 08/07/15 09:08 AM (8 years, 5 months ago)

Quote:

Munchauzen said:
I PC my plates for 90 minutes. Never had any caramalization.



:orry:


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InvisibleMad Season
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Registered: 09/16/12
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Re: germination problem [Re: Psilosoulful]
    #22057428 - 08/07/15 09:13 AM (8 years, 5 months ago)

Lol gw and flakes isn't malt. In the link I provided, malt was the only one mentioned that you had to worry about caramelization. If we used honey (which you totally can), it too can caramelize. I generally pc gw for 45, though I know 30 minutes is more than enough.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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