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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
bodhisatta said: doesn't matter it's the same agar. I know pasty says 45 but 15 would do it too. it doesn't matter you don't want your agar bottle on the bottom of the PC either.
I've never sterilized my home made or pre mix agar longer than 20m. and also never had a problem with the agar itself,
your PC takes longer to get to pressure when full, it doesn't get to 15 until things are up to temp on the inside, it's a property of the latent heat transfer of steam, it condenses on the cooler jars and looses a insane amount of heat energy until the jars heat up it won't get to 15psi. you start the timer at 15psi.
regardless of how much agar is in your PC it will still take 15m in a 500ml jar or several small pasty plates.
Thanks for the info bod, I noticed other cultivators complaining of too much condensation in their plates, agar being too stiff, etc. It is probably because PC times are too long.
And also, when I made my agar substitute tek (brf and water), I PC'd for 15 min at 15 PC and all my brf paste jars came out good with only one contaminant out of 6 jars (probably due to lack of proper sterile technique), still a success IMO.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
MA - Care must be taken used with malt not to exceed 10 pounds pressure for 20 minutes. Higher temperatures cause malt sugars to caramelize, preventing the agar from jelling properly.
http://www.shroomery.org/8506/Culture-Mediums
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TEAMSESH
Stranger


Registered: 04/08/15
Posts: 203
Last seen: 8 years, 4 months
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didn't sterilize the plates. only the mea in a mason jar got serialized at some 15-16 psi
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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I'm confused, are you saying that 10 psi is recommended for pasty plates then? Never made them before, so I don't know how they look like after a PC cycle. Just want to make sure I do it right the first time.
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TEAMSESH
Stranger


Registered: 04/08/15
Posts: 203
Last seen: 8 years, 4 months
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Tanks ill give it a read
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TEAMSESH
Stranger


Registered: 04/08/15
Posts: 203
Last seen: 8 years, 4 months
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Re: germination problem [Re: TEAMSESH]
#22055503 - 08/06/15 08:44 PM (8 years, 5 months ago) |
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great info hear I'm learning
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
Psilosoulful said: I'm confused, are you saying that 10 psi is recommended for pasty plates then? Never made them before, so I don't know how they look like after a PC cycle. Just want to make sure I do it right the first time.
15 is fine. It's just pulled from a lab book. It's just saying you don't want to overcook it. As bod said 15-20 minutes is fine. Going over 20 minutes, especially with malt agar would cause it to caramelize
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TEAMSESH
Stranger


Registered: 04/08/15
Posts: 203
Last seen: 8 years, 4 months
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hey so the food grade agar im using is in a block forum. weighs 10grams should I figure my own ratio to get it percept because don't know any thing that works with this agar .I've used powder in the past with 100% successes even brf agar style teks with successes. im using light malt extract to.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Mad Season said:
Quote:
Psilosoulful said: I'm confused, are you saying that 10 psi is recommended for pasty plates then? Never made them before, so I don't know how they look like after a PC cycle. Just want to make sure I do it right the first time.
15 is fine. It's just pulled from a lab book. It's just saying you don't want to overcook it. As bod said 15-20 minutes is fine. Going over 20 minutes, especially with malt agar would cause it to caramelize
Awesome, can't wait to mix up some agar!!
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TEAMSESH
Stranger


Registered: 04/08/15
Posts: 203
Last seen: 8 years, 4 months
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If carmalization is to occur what effects would it have on germination?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: germination problem [Re: TEAMSESH]
#22056536 - 08/07/15 02:53 AM (8 years, 5 months ago) |
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I PC my plates for 90 minutes. Never had any caramalization. Also never used malt dextrose extract. I do standard potato flakes or grain water recipes. they look the same before pc as they do after. you can pour them and let them cool down to look at them before you load them. mine usually have a nice glossy sheen to them.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Munchauzen said: I PC my plates for 90 minutes. Never had any caramalization.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Lol gw and flakes isn't malt. In the link I provided, malt was the only one mentioned that you had to worry about caramelization. If we used honey (which you totally can), it too can caramelize. I generally pc gw for 45, though I know 30 minutes is more than enough.
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