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Invisiblekeyeghost
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Registered: 09/04/02
Posts: 123
g2g spawn more quarts?
    #2204879 - 12/28/03 12:43 AM (13 years, 4 months ago)

if one were growing shiitakes on millett and wanted to use one of 85% colonized quarts to spawn more quarts my friend assumed this can be done in sterile conditions. Or, would the growth in the jars spawned from a previous jar be too slow reproducing, therefore making room for more contams?

My friends thank anyone and everyone.


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"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


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InvisibleYidakiMan
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Re: g2g spawn more quarts? [Re: keyeghost]
    #2204904 - 12/28/03 01:07 AM (13 years, 4 months ago)

Just wait until they are done.


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Invisibleangryshroom
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Re: g2g spawn more quarts? [Re: keyeghost]
    #2204996 - 12/28/03 02:07 AM (13 years, 4 months ago)

You'll want to wait.

I mean, you could do it, but, your contamination probability goes way up !


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Invisiblekeyeghost
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Re: g2g spawn more quarts? [Re: angryshroom]
    #2205039 - 12/28/03 02:42 AM (13 years, 4 months ago)

You think the risk runs too high even in a dual HEPA environment? A character in a book I am reading was also wondering about doing the same thing with agar and petri dishes, but does this have to be fresh roomie stem and not mycelium?
Thanks again.


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Offlineflow
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Re: g2g spawn more quarts? [Re: keyeghost]
    #2205166 - 12/28/03 04:26 AM (13 years, 4 months ago)

if you do the g2g in a sterile invironement then it will be fine.


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OfflineSuby
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Re: g2g spawn more quarts? [Re: keyeghost]
    #2205569 - 12/28/03 01:33 PM (13 years, 3 months ago)

of course there is more risk b/c your exsposing that ~15% to contams. but in the environment you describe shouldn't be much more risk. do it.
there shouldn't be any difference in the rapididy of the colonizing jar g1 and g2. if anything they should grow more quickly than starting from spores.
btw I though shittakes were wood loving not grain fungi??


--------------------
"Why don't they make the whole plane out of that black box stuff."
Steven Wright.

Some say the glass is half empty, some say the glass is half full, I say, are you going to drink that?

"I'm desperately trying to figure out why kamikaze pilots wore helmets."


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Invisiblekeyeghost
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Re: g2g spawn more quarts? [Re: Suby]
    #2205770 - 12/28/03 03:43 PM (13 years, 3 months ago)

those were some strange shiitakes my friend, they sure love grain.
thanks for the advice


--------------------
"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


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