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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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first time reishi
#22039841 - 08/03/15 09:06 PM (8 years, 5 months ago) |
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I'm getting a syringe from Sporeworks shortly. I will use it to inoculate quart jars of sterilized grain--I have some rye berries that I can use--but would WBS be better?
I don't know if I will do an invitro fruiting or try to inoculate bags of sterilized shredded hardwood. Probably I will try both.
Then if all goes well I will try to isolate some clean mycelium and make a new syringe and go again. Might even try to germinate some spores.
This my first attempt with this species. Does anybody know anything that I probably don't that might help me to keep my boat afloat?
Yes, I have been searching the site for days on this subject. But anyone speaking from personal experience would be nice to hear from.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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nooberst
Stranger



Registered: 03/03/15
Posts: 459
Last seen: 5 months, 7 days
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I read spores are difficult to germinate
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 3 months, 19 days
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Re: first time reishi [Re: nooberst]
#22043243 - 08/04/15 05:10 PM (8 years, 5 months ago) |
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Apparently spores from the G.resinaceum clade are easier to germinate, probably due to a thinner spore wall, The sporeworks strain might or might not be in this clade.
I do think that you might be able to germinate other species by using more spores on a plate, the more spores the better the chance presumably. But I haven't anything like that yet.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 3 months, 19 days
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Re: first time reishi [Re: Mrcloudy]
#22043254 - 08/04/15 05:12 PM (8 years, 5 months ago) |
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Both rye and WBS work well for spawn.
Reishi seems to grow well on striaght WBS, I have not tried it on straight rye. The reishi in the jars in my signature are growing on nothing but WBS.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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Hitsuzen
Space Cowboy



Registered: 10/17/08
Posts: 399
Loc: Earth
Last seen: 3 years, 2 months
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Re: first time reishi [Re: nooberst]
#22047686 - 08/05/15 02:31 PM (8 years, 5 months ago) |
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Quote:
nooberst said: I read spores are difficult to germinate
I'm pretty sure the reishi syringes from Sporeworks are liquid culture, not spores.
-------------------- Hate never yet dispelled hate, Only love dispels hate. This is the law, Ancient and inexhaustible. -Buddha
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Hitsuzen
Space Cowboy



Registered: 10/17/08
Posts: 399
Loc: Earth
Last seen: 3 years, 2 months
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Re: first time reishi [Re: Mrcloudy]
#22047725 - 08/05/15 02:35 PM (8 years, 5 months ago) |
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Quote:
Mrcloudy said: Both rye and WBS work well for spawn.
Reishi seems to grow well on striaght WBS, I have not tried it on straight rye. The reishi in the jars in my signature are growing on nothing but WBS.
What kind of yields are you getting with straight WBS vs. spawning it?
-------------------- Hate never yet dispelled hate, Only love dispels hate. This is the law, Ancient and inexhaustible. -Buddha
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nooberst
Stranger



Registered: 03/03/15
Posts: 459
Last seen: 5 months, 7 days
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Re: first time reishi [Re: Hitsuzen]
#22050528 - 08/05/15 09:56 PM (8 years, 5 months ago) |
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Quote:
Hitsuzen said:
Quote:
nooberst said: I read spores are difficult to germinate
I'm pretty sure the reishi syringes from Sporeworks are liquid culture, not spores.
They said they would try to germinate some spores
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Hitsuzen
Space Cowboy



Registered: 10/17/08
Posts: 399
Loc: Earth
Last seen: 3 years, 2 months
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Re: first time reishi [Re: nooberst]
#22050554 - 08/05/15 10:02 PM (8 years, 5 months ago) |
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Ah, I missed that part.
-------------------- Hate never yet dispelled hate, Only love dispels hate. This is the law, Ancient and inexhaustible. -Buddha
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: first time reishi [Re: Mrcloudy]
#22055964 - 08/06/15 10:39 PM (8 years, 5 months ago) |
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Quote:
Mrcloudy said: Apparently spores from the G.resinaceum clade are easier to germinate, probably due to a thinner spore wall, The sporeworks strain might or might not be in this clade.
I do think that you might be able to germinate other species by using more spores on a plate, the more spores the better the chance presumably. But I haven't anything like that yet.
I don't really care that much if I can or can't germinate spores. I can always clone a good specimen--no? I would certainly want to find some way to avoid sending more money to vendors than I have to to keep my reishi supply up.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: first time reishi [Re: Mrcloudy]
#22055969 - 08/06/15 10:41 PM (8 years, 5 months ago) |
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Quote:
Mrcloudy said: Both rye and WBS work well for spawn.
Reishi seems to grow well on striaght WBS, I have not tried it on straight rye. The reishi in the jars in my signature are growing on nothing but WBS.
I have already got 6 jars of rye grain sterilized, so tomorrow I shoot them with the sporeworks myc.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 3 months, 19 days
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Quote:
champinhom said:
Quote:
Mrcloudy said: Apparently spores from the G.resinaceum clade are easier to germinate, probably due to a thinner spore wall, The sporeworks strain might or might not be in this clade.
I do think that you might be able to germinate other species by using more spores on a plate, the more spores the better the chance presumably. But I haven't anything like that yet.
I don't really care that much if I can or can't germinate spores. I can always clone a good specimen--no? I would certainly want to find some way to avoid sending more money to vendors than I have to to keep my reishi supply up.
All you really have to do is put a drop of the liquid culture from the syringe on agar, a few plates and now you have the strain that you can expand whenever you want. No need to wait for a fruit body to clone.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: first time reishi [Re: Mrcloudy]
#22059255 - 08/07/15 03:38 PM (8 years, 5 months ago) |
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 resinaceium germinates quickly on agar. and does fine on straight rye, lucidium I have not tried to germinate spores at all but it does well on straight rye. Yields are very condition and strain dependent.
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