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Offlinemushheed
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Possible contamination during colonization?
    #22037922 - 08/03/15 02:26 PM (8 years, 5 months ago)

Hi there. Just like to state this is my first time so maybe I'm being paranoid but would like an expert opinion for my peace of mind!

Basically I've got 2 jars using the pf tek. Both inoculated at the same time. First jar, colonization started at all 4 holes and is nearly fully colonized.

Second jar, saw nothing for nearly 12 days then colonization occurred at just one of the innoculating points and it's now half done (almost twice the time).

My concern is that the jar appears to be colonizing ok however there is like brownish condensation like the verm has been pushed on to the glass. Will this cause contamination or should it be ok?

First pic is the full one. 2nd and 3rd pic are the "coloured" patches.

Thanks





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InvisibleMad Season
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Re: Possible contamination during colonization? [Re: mushheed]
    #22038215 - 08/03/15 04:03 PM (8 years, 5 months ago)

The yellow and the wet looking verm pressed up is from bacteria. Let it be and let it colonize. Chances are you'll still get fruits. If you want to really look into detail, read these threads I gave out all my knowledge on bacteria with:

http://www.shroomery.org/forums/showflat.php/Number/21936535
http://www.shroomery.org/forums/showflat.php/Number/21744946
http://www.shroomery.org/forums/showflat.php/Number/22029196

You got it because it was hitching a ride in the syringe (even if it was from sponsors it still can) or it was from improper procedure/sterilization.

Check out my improve your sterile techniques link in my signature to see how I started off clean, so that I know when I get bacteria, it's because of improper sterilization.

Hope this helps :smile:


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Edited by Mad Season (08/03/15 04:05 PM)


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Offlinebluegill
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Re: Possible contamination during colonization? [Re: mushheed]
    #22038217 - 08/03/15 04:04 PM (8 years, 5 months ago)

Yellow blotch in 3 is bacteria. I have had cakes with that that have fruited. In fact I have one fruiting right now. When you have a bunch of spores, you never know what you're going to get. Each jar will have different growth rates and so on.


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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Offlinemushheed
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Re: Possible contamination during colonization? [Re: Mad Season]
    #22040495 - 08/04/15 02:12 AM (8 years, 5 months ago)

Some really useful info that I've not came across on here yet which I'll definitely use!

I tried and failed twice before these and this is the first time I took extra care to make sure everything was done right so I will hope for the best!

Is there anything I should do different or key things to look out for it going bad? For example is it still ok to fruit both cakes in the same chamber?

Thanks for the replies


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Offlinebluegill
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Re: Possible contamination during colonization? [Re: mushheed]
    #22043488 - 08/04/15 05:50 PM (8 years, 5 months ago)

Yes it's ok to fruit in chamber with uncontaminated cakes. Make sure to wash it really good when you birth and dunk it. It should be fine. I'd also take extra precaution and wait to eat the fruits when they are dry.


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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Offlinemushheed
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Re: Possible contamination during colonization? [Re: bluegill]
    #22048168 - 08/05/15 03:54 PM (8 years, 5 months ago)

Thanks for the advice. Hopefully will be ok :smile:


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