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Aureus
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Registered: 07/04/15
Posts: 478
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Is it ok if I use polyfill during incubation?
#22037913 - 08/03/15 02:23 PM (8 years, 5 months ago) |
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My monotubs have been having a lot of contam issues. The contams always happen during the incubation period. I suspect it is because of the heat and stale air.
Should I allow fresh air during incubation?
Edited by Aureus (08/03/15 02:25 PM)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Is it ok if I use polyfill during incubation? [Re: Aureus]
#22037940 - 08/03/15 02:32 PM (8 years, 5 months ago) |
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no. I wouldn't. always think of environmental controls as the room & not just the tub (you mentioned temp problem?)
1) ask in the proper forum for better results (mushroom cultivation) 2) check your pasteurization method is up to date (I use user MudaFuka's Improved bucket tek for coir&verm substrate pasteurization) 3) if your substrate is contaminating that quickly also make sure your spawn is clean, I recommend doing lots of trials (start from different spores). Also a big help is agar.
pasteurized coir/verm should last a while. the time for a monotub to colonize would be like max two weeks.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Aureus
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Registered: 07/04/15
Posts: 478
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Re: Is it ok if I use polyfill during incubation? [Re: filthyknees]
#22037990 - 08/03/15 02:48 PM (8 years, 5 months ago) |
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I pasteurize the coco coir for 30 minutes in the pressure cooker, that's probably not the problem.
I'm certain that if I up the fresh air the contamination rate will reduce drastically. What i'm actually worried is if do this during the incubation I will have early pinning.
Edited by Aureus (08/03/15 03:00 PM)
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tryptkaloids
Learner



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Re: Is it ok if I use polyfill during incubation? [Re: Aureus] 1
#22038014 - 08/03/15 02:56 PM (8 years, 5 months ago) |
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ask on the proper forum
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Is it ok if I use polyfill during incubation? [Re: Aureus]
#22038176 - 08/03/15 03:53 PM (8 years, 5 months ago) |
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yeah pretty much you will have early pinning if there is fae. most likely it wont even fully colonize and put out immature fruits out of the substrate which will be weak because it wont be at 100% which means it wont get past the first flush. with that.
if it's not your substrate it is your spawn(sterile procedure). dunno how removing one holes tape will fix that. Never seen that as a practice to reduce contam. I've seen it done - when colonizing tubs are stored in a draft free space - nothing to do with reducing contams. :\
and are you sure it isn't your substrate cause it sounds like you are taking shots in the dark without posting your procedure. I pasteurize mine for about an hour. What tek are you using to pasturize? one from 2001? post a link to it & maybe get better responses.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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healing
Strangest



Registered: 02/22/11
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Re: Is it ok if I use polyfill during incubation? [Re: Aureus]
#22040668 - 08/04/15 04:56 AM (8 years, 5 months ago) |
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Quote:
Aureus said: I pasteurize the coco coir for 30 minutes in the pressure cooker, that's probably not the problem.
I'm certain that if I up the fresh air the contamination rate will reduce drastically. What i'm actually worried is if do this during the incubation I will have early pinning.
No. You are misunderstanding the life-cycle of the contaminants. They have to incubate, just like your mycelium. Your contaminants are in the substrate long before they become apparent. Increasing the air exchange will cause early pinning and will introduce another vector for contamination.
I think your problem might be that you are pressure cooking your substrate. If I remember correctly, you are supposed to pasteurize your colr, not sterilize it. It's been a long time since I've grown, so take this with a grain of salt, ask in the proper forum, and if you don't mind, give us a link so that we can find out what the people over at mushcult say.
-------------------- Open mind, open heart, open book.
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Aureus
Stranger
Registered: 07/04/15
Posts: 478
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Re: Is it ok if I use polyfill during incubation? [Re: healing]
#22041249 - 08/04/15 09:40 AM (8 years, 5 months ago) |
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Bacterias are kind of resistant to heat, 30 minutes in the pressure cooker won't even tickle them  But you're right, sterilization is very bad when it comes to bulks.
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psilocybinjunkie
relaxin


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Re: Is it ok if I use polyfill during incubation? (moved) [Re: Aureus]
#22041283 - 08/04/15 09:54 AM (8 years, 5 months ago) |
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This thread was moved from The Psychedelic Experience.
Reason: cult related.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Is it ok if I use polyfill during incubation? [Re: Aureus]
#22042476 - 08/04/15 02:48 PM (8 years, 5 months ago) |
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Quote:
Aureus said: My monotubs have been having a lot of contam issues. The contams always happen during the incubation period. I suspect it is because of the heat and stale air.
Should I allow fresh air during incubation?
contaminated or majorly bacterial spawn.
to answer you Q directly, yes you can stuff the holes real tight with polyfil for colonization incubation isnt necessary, you'll have better chances just letting in colonize in room temps!
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Aureus
Stranger
Registered: 07/04/15
Posts: 478
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Re: Is it ok if I use polyfill during incubation? [Re: spacechildo]
#22044821 - 08/04/15 10:00 PM (8 years, 5 months ago) |
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thanks
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spacechildo
proletarians rise up



Registered: 01/24/13
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Loc: Babylon
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Re: Is it ok if I use polyfill during incubation? [Re: Aureus]
#22044830 - 08/04/15 10:02 PM (8 years, 5 months ago) |
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but no fae during colonization dude. just re read my post and figured i'd make it clear.
your problem is dirty spawn. just keep your poly for fruiting.
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