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DinkinFlicka84
Dude



Registered: 01/19/14
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G2G isn't colonizing after 5 days. Stalled?
#22035408 - 08/02/15 09:25 PM (8 years, 5 months ago) |
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I did a G2G transfer 5-6 days ago and normally the mycelium starts showing at day 2 or 3. I even used a generous amount of colonized WBS in each jar. I used 2 different quarts of the same strand, so it shouldn't be that the spawn is bad, unless somehow how both jars of spawn were bad. Even if the spawn was bad, it should still colonize the new jar, right?
I made sure to dry the seeds on towels after 24 hr soak where they are not sticky to the touch and rattle in the jar. I'm fairly certain that all of WBS is hydrated, because they were sticking to the jar a little after PCing.
I can see a couple of seeds in some jars that have myc on them, but not fuzzy myc that shows actual expansion of mycelium like it normally would. My technique didn't change, which was using a SAB and spraying it down with soapy water and spraying an oust-like spray in the SAB to neutralize air-borne contams.
Any thoughts on the cause or solution?
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ShroominMe
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22035594 - 08/02/15 10:12 PM (8 years, 5 months ago) |
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I don't know the cause but with a G2G you will normally have full colonization after 5 days. Even if you don't have fae it should colonize before it stalled.
Either there was something wrong with the wbs or you got some Oust into the sub. How long did you wait after spraying with the disinfectant?
I'd start over, sorry.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: ShroominMe]
#22035610 - 08/02/15 10:15 PM (8 years, 5 months ago) |
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Any pics of your jars? Contams seem likely at this point.
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DinkinFlicka84
Dude



Registered: 01/19/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
#22036360 - 08/03/15 04:38 AM (8 years, 5 months ago) |
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I can send pics but I waited the same time i normally do. 15 min before I go into the SAB.
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blindingleaf
blue collar underworld


Registered: 07/19/13
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22036405 - 08/03/15 05:30 AM (8 years, 5 months ago) |
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Quote:
I can see a couple of seeds in some jars that have myc on them, but not fuzzy myc that shows actual expansion of mycelium like it normally would.
sounds like bacteria. post a pic.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Psilosoulful

Registered: 09/05/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22036636 - 08/03/15 08:28 AM (8 years, 5 months ago) |
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Quote:
DinkinFlicka84 said: I made sure to dry the seeds on towels after 24 hr soak where they are not sticky to the touch and rattle in the jar. I'm fairly certain that all of WBS is hydrated, because they were sticking to the jar a little after PCing.
I think not simmering your seeds after the 24 hour soak might be the issue here. Although the seeds may have look hydrated on the outside, you want to make sure the inside of the grain is hydrated and can retain moisture. The grains will expand in size as you simmer them (15 minutes is perfect) and will look a bit puffy. Then cut the heat before you get bursted kernels which will turn into a starchy mess and clump together.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
#22036816 - 08/03/15 09:37 AM (8 years, 5 months ago) |
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Quote:
Psilosoulful said:
Quote:
DinkinFlicka84 said: I made sure to dry the seeds on towels after 24 hr soak where they are not sticky to the touch and rattle in the jar. I'm fairly certain that all of WBS is hydrated, because they were sticking to the jar a little after PCing.
I think not simmering your seeds after the 24 hour soak might be the issue here. Although the seeds may have look hydrated on the outside, you want to make sure the inside of the grain is hydrated and can retain moisture. The grains will expand in size as you simmer them (15 minutes is perfect) and will look a bit puffy. Then cut the heat before you get bursted kernels which will turn into a starchy mess and clump together.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Psilosoulful

Registered: 09/05/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22036945 - 08/03/15 10:20 AM (8 years, 5 months ago) |
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I prepped these last night, this is after 15 min on a light simmer. Notice how the seeds are all puffed up. At this point I cut the heat, strain them (don't rinse them, you want the steam to help dry the grains), and let them sit on a window screen until the outside is mostly dry ( I use the toilet-paper test). Then load em up and you're ready to PC!! 
Edited by Psilosoulful (08/03/15 10:21 AM)
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DinkinFlicka84
Dude



Registered: 01/19/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
#22039059 - 08/03/15 07:03 PM (8 years, 5 months ago) |
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Sorry, I forgot to mention that I simmered for 10 min as well. Any other possible causes? The jar I used the G2G with was 100% colonized and looked very health. I've been doing this for about 2 years, so I'm fairly certain what looks healthy. I know that it's possible for trich to hide, but none of the jars have any mold. They're just sitting there with nothing happening. And I use 2 diff. Jars to do G2G with so as to make sure if one was bad then the other would make up for it.
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blindingleaf
blue collar underworld


Registered: 07/19/13
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22039069 - 08/03/15 07:07 PM (8 years, 5 months ago) |
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bacteria dude.
grain prep method doesn't really mean much. u can get a contam with any method.
excess moisture…kinda bad excess incubation heat….kinda bad
both together, really bad.
thats if ur donor jar is clean.
if ur donor jar is bacterial, nothing can save it.
agar>g1 most important step if u expanding grains. do not compromise. spawn iffy jars instead of expanding.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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9mmNATO
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22039085 - 08/03/15 07:10 PM (8 years, 5 months ago) |
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Most likely a moisture issue, or bacterial contamn. LC culture is much better way to inoculate jars.
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blindingleaf
blue collar underworld


Registered: 07/19/13
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22039089 - 08/03/15 07:11 PM (8 years, 5 months ago) |
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Quote:
9mmNATO said: Most likely a moisture issue, or bacterial contamn. LC culture is much better way to inoculate jars.
yes, because that solves both the moisture issue, and the bacteria issue!! genius
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22039093 - 08/03/15 07:12 PM (8 years, 5 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Psilosoulful

Registered: 09/05/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22039103 - 08/03/15 07:13 PM (8 years, 5 months ago) |
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9mmNATO
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22039108 - 08/03/15 07:14 PM (8 years, 5 months ago) |
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I dont know if you are being smart or whatever. The common logical solution would to be start over. Lol you act like as if theres a solution to doomed fucked up jars. I threw in the LC suggestion because next time it will be even easier because of the higher volume of mycelium and multiple points of inoculation the jar will colonize quicker giving the guy some looser tolerances for fucking up the said moisture and bacterial issues.
GENIUS.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22039119 - 08/03/15 07:16 PM (8 years, 5 months ago) |
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And if he's having jar problems you think he's going to pull off a clean LC?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22039128 - 08/03/15 07:18 PM (8 years, 5 months ago) |
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LC FASTER THEN G2G.....
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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9mmNATO
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22039132 - 08/03/15 07:18 PM (8 years, 5 months ago) |
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Of course he can.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22039137 - 08/03/15 07:18 PM (8 years, 5 months ago) |
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You're new here 9mm.
Welcome to mush cult.
Do some reading
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO] 1
#22039145 - 08/03/15 07:19 PM (8 years, 5 months ago) |
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IMO, an LC is another vector.
take out grain prep vector, take out possibly contaminated innoculant vector, and then I'm 100% in support of liquid cultures
i agree, they are fastest way to colonize. but if i was having problems, that would be one of the first things i eliminate until i narrowed down what else it could be.
yea, i was being smart. beer…u know? take me with a grain of salt tonight, i promise, i m as laid back as they come.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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9mmNATO
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
#22039149 - 08/03/15 07:20 PM (8 years, 5 months ago) |
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You obviously have no idea how to noc up a jar with an LC. maybe you have shit genetics going on but LC for me usually takes 4 days to colonize a quart jar. I make the jars somewhat drier and use a lot of LC all over the jar and shake well. You obviously also dont grasp the concept of surface area.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22039158 - 08/03/15 07:22 PM (8 years, 5 months ago) |
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Quote:
You obviously have no idea how to noc up a jar with an LC
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf] 1
#22039165 - 08/03/15 07:23 PM (8 years, 5 months ago) |
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I love new guys.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Psilosoulful

Registered: 09/05/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22039176 - 08/03/15 07:24 PM (8 years, 5 months ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful] 1
#22039192 - 08/03/15 07:28 PM (8 years, 5 months ago) |
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old redundant arguement i want no part of, i love lc's and i will leave it at that
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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9mmNATO
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
#22039456 - 08/03/15 08:07 PM (8 years, 5 months ago) |
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Just because im new to a message board means im inexperienced. Using stupid little gifs instead of facts. Not to be rude but how immature.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22039497 - 08/03/15 08:18 PM (8 years, 5 months ago) |
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Same happened to me.. I have 10 jars that I inoculated with spores. No growth after over a week. Waiting on agar to colonize so I can just use a clean plate.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO] 1
#22039540 - 08/03/15 08:21 PM (8 years, 5 months ago) |
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you're the only up tight one here if you prefer lc's all the power to you, for cubes i'm a g2g guy, but if i took a plate of cube myc and tossed a chunk in lc and a chunk in some masters for g2g the g2g wins everytime, if ya got a whack of lc already ready to use go for it but as bl stated it has it's own risks, you wanna argue any points go for it your not changing my mind though lol, again i love lc and use it alot btu if ya can't take the odd joke your not likely to last here
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22040699 - 08/04/15 05:11 AM (8 years, 5 months ago) |
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So, I used to do LC, but that caused a HUGE moisture problem. I had contams for 6 months before my first flush on EVERY SINGLE SUB.
When I switched to G2G I was doing 2 lbs/mo. I was ecstatic. It's my 4th month of doing G2G now and this last G2G was a waste. It's day 7 and only 1 jar has a tiny bit of myc in it out 32 total. I know I shouldn't do so many at a time, but I was feeling confident in my procedure since my agar was going well, my G2G was going well, and not one contaminated jar or sub for 4 months since I switched to G2G. LC is for pros only IMO. G2G is fast as hell at about 6-10 days for 100% colonization. This depends on how much colonized grain you put in each jar of course. I take one jar of spawn and spread it out to 12 jars, which has worked for a while.
I can't believe that almost every since jar didn't colonize at all. The WBS was completely dry to the touch, which is the way I like it, after having too much moisture in my jars before and getting bacteria EVERY TIME.
I ordered Burma and B+, so I'll be starting over again, except I'll probably inoculate some agar plates first and clean them up before I go to grain.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22040715 - 08/04/15 05:19 AM (8 years, 5 months ago) |
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Like I said I've used LC, but how can you say that LC is faster than G2G? The mycelium is already colonized somewhat on the grains itself, instead of LC, where the myc is in a primal stage of growth, which takes longer. My G2G is 6-10 days, but I've never had LC be faster than that. Ever. Plus, why would I want to mess with moisture content by adding liquid to my jar? My WBS is hydrated already, so the stage is set for colonization.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22040721 - 08/04/15 05:21 AM (8 years, 5 months ago) |
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LC and G2G pretty similar speed, both are based on inoc rate. if u use 2ml per jar, thats slower than 10ml per jar if u g2g 1 qt to six qts, thats faster than g2g>ten qts.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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9mmNATO
Stranger

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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22040888 - 08/04/15 06:37 AM (8 years, 5 months ago) |
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Quote:
blindingleaf said: LC and G2G pretty similar speed, both are based on inoc rate. if u use 2ml per jar, thats slower than 10ml per jar if u g2g 1 qt to six qts, thats faster than g2g>ten qts.
Thank you.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
#22040930 - 08/04/15 07:07 AM (8 years, 5 months ago) |
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 no problem bro, good luck.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
#22045647 - 08/05/15 02:55 AM (8 years, 5 months ago) |
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I know I should just start over, which I will in a couple of days. However, I would like try and do an LC, since I have a few 100% jars and feel that the 30 something jars should still be fine. Only 3 or 4 have some dusty looking mold growing, which I've experienced before.
The rest of my jars (w/ exception of 2), look just like they did before I did G2G. I still can't figure out why they didn't take when I used a decent amount of spawn when doing G2G, as to speed up the process. Some I did with a small amount of spawn also.
Is it worth trying to inoculate with LC, as long as I use a tiny squirt per jar? Previously, I would use 1-2cc per jar, but that would cause bacteria every time I would make my sub and the bacteria came in contact with the FAE and destroyed ALL of my subs before a flush began. This has caused me to fear doing LC's in general, since G2G was so easy and quicker IME. I know that some people use 2cc per jar when inoculating and have no problems with contams, so I wonder what I've done wrong? I know that my moisture content must've caused the contaminants.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22047294 - 08/05/15 12:54 PM (8 years, 5 months ago) |
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Quote:
DinkinFlicka84 said:
The rest of my jars (w/ exception of 2), look just like they did before I did G2G. I still can't figure out why they didn't take when I used a decent amount of spawn when doing G2G, as to speed up the process. Some I did with a small amount of spawn also.
Bacteria. Open em up and see if they smell funny.
I would suggest agar>grain over your Liquid Contaminant. Or test your LC on agar first. Either way, next best step: Agar.
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prismism


Registered: 05/11/09
Posts: 5,570
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22050758 - 08/05/15 10:44 PM (8 years, 5 months ago) |
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Quote:
taGyo said: You're new here 9mm.
Welcome to mush cult.
Do some reading 
You're new here too. What's with the superiority complex? 9mm was giving out solid information and not trying to start shit like you are. All you've contributed to this thread is guff and some silly gifs.
-------------------- ephemeral anomalous
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism]
#22050777 - 08/05/15 10:48 PM (8 years, 5 months ago) |
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And you brought? Lol...9 mm was banned and if the jars have not recovered i would say contam
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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prismism


Registered: 05/11/09
Posts: 5,570
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
#22050795 - 08/05/15 10:51 PM (8 years, 5 months ago) |
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I'm just here to regulate. Also, Dink is my good friend outside of this forum.
Hope you get this issue cleared up, bud. It does sound like bacteria without any photographic help. Are you still working with agar?
-------------------- ephemeral anomalous
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism] 1
#22050816 - 08/05/15 10:54 PM (8 years, 5 months ago) |
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Atta guy
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
#22051224 - 08/06/15 12:17 AM (8 years, 5 months ago) |
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Quote:
cronicr said: your not likely to last here
Quote:
cronicr said: .9 mm was banned
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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prismism


Registered: 05/11/09
Posts: 5,570
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
#22051359 - 08/06/15 12:53 AM (8 years, 5 months ago) |
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The endless headbutting and kneejerking drama is why I always come crawling back to mush cult.
Shroomz are serious bidness
-------------------- ephemeral anomalous
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism] 1
#22051403 - 08/06/15 01:06 AM (8 years, 5 months ago) |
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You know 9 mm really wasn't giving good advice. Switching to liquids when you are having contamination problems is pretty silly. I believe the advice he should have said was to try out agar and to look at the improve your sterile techniques link in my signature 
Can't believe he's banned already lol. It seems pretty hard to get banned. Minus the whole ban lottery lol
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Mad Season]
#22051405 - 08/06/15 01:07 AM (8 years, 5 months ago) |
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he was a puppet lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism]
#22052874 - 08/06/15 10:56 AM (8 years, 5 months ago) |
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Quote:
Mad Season said: You know 9 mm really wasn't giving good advice. Switching to liquids when you are having contamination problems is pretty silly. I believe the advice he should have said was to try out agar and to look at the improve your sterile techniques link in my signature 
Can't believe he's banned already lol. It seems pretty hard to get banned. Minus the whole ban lottery lol

Quote:
prismism said: The endless headbutting and kneejerking drama is why I always come crawling back to mush cult.
Shroomz are serious bidness
Just cause you registered here six years ago doesn't mean you know shit . I've seen people who registered in 06 asking shit like how do I do agar.
Enjoy yourself
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22061471 - 08/08/15 03:20 AM (8 years, 5 months ago) |
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Guess ole' Pris has got my back.
Here's some pics to help. I agree with the bacteria/contaminants. Most of my jars have this dusty look and I opened one and smelled, which smelled like an old dusty...something or other. Not as potent smelling as a moldy jar, but def. not a normal WBS smell.
My biggest question is what caused it. Is it possible for my WBS to be too dry and that would cause it to not colonize? I've done this G2G process 4 times now in my SAB and I didn't change anything about my procedure, which is why I'm still scratching my head. These pics were just taken, which is about 10 days since I did the G2G. Remember, I used 2 different 100% colonized jars for this, so that if one jar was somehow bad, the other would most likely work.
The 4th pic from the left has some colonization, but it's stalled for sure.
Also, I just got in Burma and B+, can I apply a drop or 2 to agar? Or does it have to be in a different form?
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Psilosoulful

Registered: 09/05/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22061757 - 08/08/15 08:00 AM (8 years, 5 months ago) |
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Those jars looked overcooked for sure. How long did you PC them and at what psi? Did you give them a soak before simmering?
And yes spores to agar would be a great choice for spotting contams before knocking up a grain jar. Less stress and much better results. A drop of MS per agar dish is fine, make sure it touches all parts of the agar. Once you see a bit of good growth, take a small edge of the outside and put that on a new dish. Check out Pastywhyte's Easy Agar tek for more info.
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Nature Boy
Stranger than most



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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22061783 - 08/08/15 08:14 AM (8 years, 5 months ago) |
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I thought all sunflower seeds had to be removed from WBS before use. I see lots of them in your jars.
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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DinkinFlicka84
Dude



Registered: 01/19/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
#22061807 - 08/08/15 08:28 AM (8 years, 5 months ago) |
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15 PSI for 90+ min. Less than 2 hours. I always do a 24hr soak to germinate/hydrate the grains.
So they may be overcooked? I also dry them out for a good while with fans and usually over night. The other 7 or 8 times I've dried them like that, I haven't had any problems. If they're over cooked or too dry, could that cause them not to colonize after a G2G? That's what I'm trying to figure out.
When I'm putting a drop from my MS from the trusted site to the agar, you said to make sure the drops touch all parts of the agar. So one drop in the middle of the plate isn't enough? Are you suggesting I put multiple drops around the plate? And yes, I use pastywhite's agar tek and also RR's DVD for every step of the hobby.
They gave me an extra MS, ChitWan, which I've never heard of. Anyone have experience with that strain? Or even Burma & B+? Are they any slower/faster, bigger/smaller fruits? I know that they are all generally the same speed/size, which the size depends on your sub depth/surface area.
My subs are about 9" x 11" x 4". I'd like to get the bigger fruits, which only seem to come at my 2nd, 3rd, and flushes. I know this is typical, but somehow there has to be a way to get large fruits on the 1st flush.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22061966 - 08/08/15 09:23 AM (8 years, 5 months ago) |
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Quote:
Nature Boy said: I thought all sunflower seeds had to be removed from WBS before use. I see lots of them in your jars.
N.B.
As far as I know they colonize just fine. I've used WBS and never removed anything. The sticks are a pain in the ass come colonization but that's about the worst of it.
Quote:
DinkinFlicka84 said: 15 PSI for 90+ min. Less than 2 hours. I always do a 24hr soak to germinate/hydrate the grains.
I think it's a combination of your camera and the sunflower seeds. I don't think they're over-cooked, you just have a great camera, kitchen lighting and black sunflower grains. They look fine. "Over-cooked" will colonize fine, maybe on the dry side so I'd recommend dunking before spawning.
Quote:
DinkinFlicka84 said: When I'm putting a drop from my MS from the trusted site to the agar, you said to make sure the drops touch all parts of the agar. So one drop in the middle of the plate isn't enough? Are you suggesting I put multiple drops around the plate? And yes, I use pastywhite's agar tek and also RR's DVD for every step of the hobby.
One drop in the middle is fine. I do two drops on either end just for double the chance of germination. So if I have a circle I'd split it in half and do one on the left and one on the right. That way if one doesn't germ or gets bacteria I have a second chance. Don't move the plate after germing immediately, give it a few minutes to stick. If you can leave it over night this is even better.
Quote:
DinkinFlicka84 said: They gave me an extra MS, ChitWan, which I've never heard of. Anyone have experience with that strain? Or even Burma & B+? Are they any slower/faster, bigger/smaller fruits? I know that they are all generally the same speed/size, which the size depends on your sub depth/surface area.
Every drop from that syringe has different possibilities. I have ChitWan, it's good 
Quote:
DinkinFlicka84 said: My subs are about 9" x 11" x 4". I'd like to get the bigger fruits, which only seem to come at my 2nd, 3rd, and flushes. I know this is typical, but somehow there has to be a way to get large fruits on the 1st flush.

Larger fruits typically happen on the second flush. This is not to say they won't happen on the first but typically the second is where you start to see prime, fat fruits. Don't worry about LARGE, worry about MANY. If you have one large 3.5 gram mushroom then you only have an eighth. If you have 30 1 gram mushrooms then you have 30 grams.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Nature Boy]
#22062012 - 08/08/15 09:37 AM (8 years, 5 months ago) |
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How have you been a member since '07 and not know that sunflower seeds are completely fine to use? I haven't picked a sunflower seed out in over 2 years. All of grows have colonized sunflower seeds.
Sorry, not trying to sound condescending, I just thought it was common knowledge for anyone growing for as long as you have.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22062018 - 08/08/15 09:38 AM (8 years, 5 months ago) |
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Some people register and don't grow for five more years.
Some people register and have already grown for several years.
Don't take dates or post, take quality of information given.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
Edited by taGyo (08/08/15 09:38 AM)
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Nature Boy
Stranger than most



Registered: 07/09/07
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22062178 - 08/08/15 10:34 AM (8 years, 5 months ago) |
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It was a QUESTION, m'kay? I'm not going to go searching for it, but I'm certain I read on this site (years ago) that sunflower seeds slowed colonization or were more prone to contamination or some such. Anyway, there was some legit reason given by the OP. But you know how it is - one person's methodology is contradicted by someone else's experience. 
I only use Rye, so it never has even been a thought for me. I simply recollected that I read it here at the Shroomery and I noted that sunflower seeds were in your WBS. Take a chill pill, will ya please? It wasn't meant as an insult, it was merely a question borne out of something I'd previously read.
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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taGyo
Strainiac/AMU



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Posts: 18,802
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Nature Boy]
#22062184 - 08/08/15 10:36 AM (8 years, 5 months ago) |
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Quote:
Nature Boy said: I read on this site (years ago) that sunflower seeds slowed colonization or were more prone to contamination or some such.
Years ago is the key term here.
Don't worry about it,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Nature Boy
Stranger than most



Registered: 07/09/07
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22062210 - 08/08/15 10:47 AM (8 years, 5 months ago) |
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LOL! no problem. I won't. Just kinda rankles when you make what passes around here for a benign post and someone still jumps down your throat.
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Nature Boy]
#22062729 - 08/08/15 01:32 PM (8 years, 5 months ago) |
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I didn't mean for the post to go sideways nor did I mean it to be so aggressive. If you re-read my phrasing, it really was a legit question. I guess because I've been on here for only a short time, but I've read so much that I've come across the sunflower thing a number of times without even searching for it. I shouldn't assume that everyone has obsessed over this site as much as a guy like myself with a load of time on his hands.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22063042 - 08/08/15 03:04 PM (8 years, 5 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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