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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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prismism


Registered: 05/11/09
Posts: 5,570
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
#22051359 - 08/06/15 12:53 AM (8 years, 5 months ago) |
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The endless headbutting and kneejerking drama is why I always come crawling back to mush cult.
Shroomz are serious bidness
-------------------- ephemeral anomalous
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism] 1
#22051403 - 08/06/15 01:06 AM (8 years, 5 months ago) |
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You know 9 mm really wasn't giving good advice. Switching to liquids when you are having contamination problems is pretty silly. I believe the advice he should have said was to try out agar and to look at the improve your sterile techniques link in my signature 
Can't believe he's banned already lol. It seems pretty hard to get banned. Minus the whole ban lottery lol
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Mad Season]
#22051405 - 08/06/15 01:07 AM (8 years, 5 months ago) |
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he was a puppet lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism]
#22052874 - 08/06/15 10:56 AM (8 years, 5 months ago) |
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Quote:
Mad Season said: You know 9 mm really wasn't giving good advice. Switching to liquids when you are having contamination problems is pretty silly. I believe the advice he should have said was to try out agar and to look at the improve your sterile techniques link in my signature 
Can't believe he's banned already lol. It seems pretty hard to get banned. Minus the whole ban lottery lol

Quote:
prismism said: The endless headbutting and kneejerking drama is why I always come crawling back to mush cult.
Shroomz are serious bidness
Just cause you registered here six years ago doesn't mean you know shit . I've seen people who registered in 06 asking shit like how do I do agar.
Enjoy yourself
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22061471 - 08/08/15 03:20 AM (8 years, 5 months ago) |
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Guess ole' Pris has got my back.
Here's some pics to help. I agree with the bacteria/contaminants. Most of my jars have this dusty look and I opened one and smelled, which smelled like an old dusty...something or other. Not as potent smelling as a moldy jar, but def. not a normal WBS smell.
My biggest question is what caused it. Is it possible for my WBS to be too dry and that would cause it to not colonize? I've done this G2G process 4 times now in my SAB and I didn't change anything about my procedure, which is why I'm still scratching my head. These pics were just taken, which is about 10 days since I did the G2G. Remember, I used 2 different 100% colonized jars for this, so that if one jar was somehow bad, the other would most likely work.
The 4th pic from the left has some colonization, but it's stalled for sure.
Also, I just got in Burma and B+, can I apply a drop or 2 to agar? Or does it have to be in a different form?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22061757 - 08/08/15 08:00 AM (8 years, 5 months ago) |
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Those jars looked overcooked for sure. How long did you PC them and at what psi? Did you give them a soak before simmering?
And yes spores to agar would be a great choice for spotting contams before knocking up a grain jar. Less stress and much better results. A drop of MS per agar dish is fine, make sure it touches all parts of the agar. Once you see a bit of good growth, take a small edge of the outside and put that on a new dish. Check out Pastywhyte's Easy Agar tek for more info.
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Nature Boy
Stranger than most



Registered: 07/09/07
Posts: 8,241
Loc: Samsara
Last seen: 2 months, 6 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22061783 - 08/08/15 08:14 AM (8 years, 5 months ago) |
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I thought all sunflower seeds had to be removed from WBS before use. I see lots of them in your jars.
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
#22061807 - 08/08/15 08:28 AM (8 years, 5 months ago) |
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15 PSI for 90+ min. Less than 2 hours. I always do a 24hr soak to germinate/hydrate the grains.
So they may be overcooked? I also dry them out for a good while with fans and usually over night. The other 7 or 8 times I've dried them like that, I haven't had any problems. If they're over cooked or too dry, could that cause them not to colonize after a G2G? That's what I'm trying to figure out.
When I'm putting a drop from my MS from the trusted site to the agar, you said to make sure the drops touch all parts of the agar. So one drop in the middle of the plate isn't enough? Are you suggesting I put multiple drops around the plate? And yes, I use pastywhite's agar tek and also RR's DVD for every step of the hobby.
They gave me an extra MS, ChitWan, which I've never heard of. Anyone have experience with that strain? Or even Burma & B+? Are they any slower/faster, bigger/smaller fruits? I know that they are all generally the same speed/size, which the size depends on your sub depth/surface area.
My subs are about 9" x 11" x 4". I'd like to get the bigger fruits, which only seem to come at my 2nd, 3rd, and flushes. I know this is typical, but somehow there has to be a way to get large fruits on the 1st flush.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22061966 - 08/08/15 09:23 AM (8 years, 5 months ago) |
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Quote:
Nature Boy said: I thought all sunflower seeds had to be removed from WBS before use. I see lots of them in your jars.
N.B.
As far as I know they colonize just fine. I've used WBS and never removed anything. The sticks are a pain in the ass come colonization but that's about the worst of it.
Quote:
DinkinFlicka84 said: 15 PSI for 90+ min. Less than 2 hours. I always do a 24hr soak to germinate/hydrate the grains.
I think it's a combination of your camera and the sunflower seeds. I don't think they're over-cooked, you just have a great camera, kitchen lighting and black sunflower grains. They look fine. "Over-cooked" will colonize fine, maybe on the dry side so I'd recommend dunking before spawning.
Quote:
DinkinFlicka84 said: When I'm putting a drop from my MS from the trusted site to the agar, you said to make sure the drops touch all parts of the agar. So one drop in the middle of the plate isn't enough? Are you suggesting I put multiple drops around the plate? And yes, I use pastywhite's agar tek and also RR's DVD for every step of the hobby.
One drop in the middle is fine. I do two drops on either end just for double the chance of germination. So if I have a circle I'd split it in half and do one on the left and one on the right. That way if one doesn't germ or gets bacteria I have a second chance. Don't move the plate after germing immediately, give it a few minutes to stick. If you can leave it over night this is even better.
Quote:
DinkinFlicka84 said: They gave me an extra MS, ChitWan, which I've never heard of. Anyone have experience with that strain? Or even Burma & B+? Are they any slower/faster, bigger/smaller fruits? I know that they are all generally the same speed/size, which the size depends on your sub depth/surface area.
Every drop from that syringe has different possibilities. I have ChitWan, it's good 
Quote:
DinkinFlicka84 said: My subs are about 9" x 11" x 4". I'd like to get the bigger fruits, which only seem to come at my 2nd, 3rd, and flushes. I know this is typical, but somehow there has to be a way to get large fruits on the 1st flush.

Larger fruits typically happen on the second flush. This is not to say they won't happen on the first but typically the second is where you start to see prime, fat fruits. Don't worry about LARGE, worry about MANY. If you have one large 3.5 gram mushroom then you only have an eighth. If you have 30 1 gram mushrooms then you have 30 grams.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Nature Boy]
#22062012 - 08/08/15 09:37 AM (8 years, 5 months ago) |
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How have you been a member since '07 and not know that sunflower seeds are completely fine to use? I haven't picked a sunflower seed out in over 2 years. All of grows have colonized sunflower seeds.
Sorry, not trying to sound condescending, I just thought it was common knowledge for anyone growing for as long as you have.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22062018 - 08/08/15 09:38 AM (8 years, 5 months ago) |
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Some people register and don't grow for five more years.
Some people register and have already grown for several years.
Don't take dates or post, take quality of information given.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
Edited by taGyo (08/08/15 09:38 AM)
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Nature Boy
Stranger than most



Registered: 07/09/07
Posts: 8,241
Loc: Samsara
Last seen: 2 months, 6 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22062178 - 08/08/15 10:34 AM (8 years, 5 months ago) |
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It was a QUESTION, m'kay? I'm not going to go searching for it, but I'm certain I read on this site (years ago) that sunflower seeds slowed colonization or were more prone to contamination or some such. Anyway, there was some legit reason given by the OP. But you know how it is - one person's methodology is contradicted by someone else's experience. 
I only use Rye, so it never has even been a thought for me. I simply recollected that I read it here at the Shroomery and I noted that sunflower seeds were in your WBS. Take a chill pill, will ya please? It wasn't meant as an insult, it was merely a question borne out of something I'd previously read.
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Nature Boy]
#22062184 - 08/08/15 10:36 AM (8 years, 5 months ago) |
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Quote:
Nature Boy said: I read on this site (years ago) that sunflower seeds slowed colonization or were more prone to contamination or some such.
Years ago is the key term here.
Don't worry about it,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Nature Boy
Stranger than most



Registered: 07/09/07
Posts: 8,241
Loc: Samsara
Last seen: 2 months, 6 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
#22062210 - 08/08/15 10:47 AM (8 years, 5 months ago) |
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LOL! no problem. I won't. Just kinda rankles when you make what passes around here for a benign post and someone still jumps down your throat.
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Nature Boy]
#22062729 - 08/08/15 01:32 PM (8 years, 5 months ago) |
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I didn't mean for the post to go sideways nor did I mean it to be so aggressive. If you re-read my phrasing, it really was a legit question. I guess because I've been on here for only a short time, but I've read so much that I've come across the sunflower thing a number of times without even searching for it. I shouldn't assume that everyone has obsessed over this site as much as a guy like myself with a load of time on his hands.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
#22063042 - 08/08/15 03:04 PM (8 years, 5 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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