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Offline9mmNATO
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Registered: 08/03/15
Posts: 28
Last seen: 8 years, 5 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
    #22039149 - 08/03/15 07:20 PM (8 years, 5 months ago)

You obviously have no idea how to noc up a jar with an LC. maybe you have shit genetics going on but LC for me usually takes 4 days to colonize a quart jar.  I make the jars somewhat drier and use a lot of LC all over the jar and shake well. You obviously also dont grasp the concept of surface area.


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
Posts: 22,008
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22039158 - 08/03/15 07:22 PM (8 years, 5 months ago)

Quote:

You obviously have no idea how to noc up a jar with an LC






--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinetaGyo
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Registered: 10/16/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf] * 1
    #22039165 - 08/03/15 07:23 PM (8 years, 5 months ago)

:canthelpbutlaugh:

I love new guys.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22039176 - 08/03/15 07:24 PM (8 years, 5 months ago)

:rofl2:


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OfflinecronicrFacebook
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful] * 1
    #22039192 - 08/03/15 07:28 PM (8 years, 5 months ago)

:lol: old redundant arguement i want no part of, i love lc's and i will leave it at that


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offline9mmNATO
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Registered: 08/03/15
Posts: 28
Last seen: 8 years, 5 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
    #22039456 - 08/03/15 08:07 PM (8 years, 5 months ago)

Just because im new to a message board means im inexperienced. Using stupid little gifs instead of facts. Not to be rude but how immature.


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OfflineGreenRabbit
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Registered: 04/28/13
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22039497 - 08/03/15 08:18 PM (8 years, 5 months ago)

Same happened to me.. I have 10 jars that I inoculated with spores. No growth after over a week. Waiting on agar to colonize so I can just use a clean plate.


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OfflinecronicrFacebook
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO] * 1
    #22039540 - 08/03/15 08:21 PM (8 years, 5 months ago)

you're the only up tight one here:shrug: if you prefer lc's all the power to you, for cubes i'm a g2g guy, but if i took a plate of cube myc and tossed a chunk in lc and a chunk in some masters for g2g the g2g wins everytime, if ya got a whack of lc already ready to use go for it but as bl stated it has it's own risks, you wanna argue any points go for it your not changing my mind though lol, again i love lc and use it alot btu if ya can't take the odd joke your not likely to last here


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineDinkinFlicka84
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Registered: 01/19/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22040699 - 08/04/15 05:11 AM (8 years, 5 months ago)

So, I used to do LC, but that caused a HUGE moisture problem. I had contams for 6 months before my first flush on EVERY SINGLE SUB.

When I switched to G2G I was doing 2 lbs/mo. I was ecstatic. It's my 4th month of doing G2G now and this last G2G was a waste. It's day 7 and only 1 jar has a tiny bit of myc in it out 32 total. I know I shouldn't do so many at a time, but I was feeling confident in my procedure since my agar was going well, my G2G was going well, and not one contaminated jar or sub for 4 months since I switched to G2G. LC is for pros only IMO. G2G is fast as hell at about 6-10 days for 100% colonization. This depends on how much colonized grain you put in each jar of course. I take one jar of spawn and spread it out to 12 jars, which has worked for a while.

I can't believe that almost every since jar didn't colonize at all. The WBS was completely dry to the touch, which is the way I like it, after having too much moisture in my jars before and getting bacteria EVERY TIME.

I ordered Burma and B+, so I'll be starting over again, except I'll probably inoculate some agar plates first and clean them up before I go to grain.


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OfflineDinkinFlicka84
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22040715 - 08/04/15 05:19 AM (8 years, 5 months ago)

Like I said I've used LC, but how can you say that LC is faster than G2G? The mycelium is already colonized somewhat on the grains itself, instead of LC, where the myc is in a primal stage of growth, which takes longer. My G2G is 6-10 days, but I've never had LC be faster than that. Ever. Plus, why would I want to mess with moisture content by adding liquid to my jar? My WBS is hydrated already, so the stage is set for colonization.


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Invisibleblindingleaf
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22040721 - 08/04/15 05:21 AM (8 years, 5 months ago)

LC and G2G pretty similar speed, both are based on inoc rate.
if u use 2ml per jar, thats slower than 10ml per jar
if u g2g 1 qt to six qts, thats faster than g2g>ten qts.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offline9mmNATO
Stranger

Registered: 08/03/15
Posts: 28
Last seen: 8 years, 5 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22040888 - 08/04/15 06:37 AM (8 years, 5 months ago)

Quote:

blindingleaf said:
LC and G2G pretty similar speed, both are based on inoc rate.
if u use 2ml per jar, thats slower than 10ml per jar
if u g2g 1 qt to six qts, thats faster than g2g>ten qts.



Thank you.


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Invisibleblindingleaf
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22040930 - 08/04/15 07:07 AM (8 years, 5 months ago)

:hatsoff:
no problem bro, good luck.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineDinkinFlicka84
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Registered: 01/19/14
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22045647 - 08/05/15 02:55 AM (8 years, 5 months ago)

I know I should just start over, which I will in a couple of days.
However, I would like try and do an LC, since I have a few 100% jars and feel that the 30 something jars should still be fine. Only 3 or 4 have some dusty looking mold growing, which I've experienced before.

The rest of my jars (w/ exception of 2), look just like they did before I did G2G. I still can't figure out why they didn't take when I used a decent amount of spawn when doing G2G, as to speed up the process. Some I did with a small amount of spawn also.

Is it worth trying to inoculate with LC, as long as I use a tiny squirt per jar? Previously, I would use 1-2cc per jar, but that would cause bacteria every time I would make my sub and the bacteria came in contact with the FAE and destroyed ALL of my subs before a flush began. This has caused me to fear doing LC's in general, since G2G was so easy and quicker IME. I know that some people use 2cc per jar when inoculating and have no problems with contams, so I wonder what I've done wrong? I know that my moisture content must've caused the contaminants.


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OfflineGreenRabbit
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22047294 - 08/05/15 12:54 PM (8 years, 5 months ago)

Quote:

DinkinFlicka84 said:

The rest of my jars (w/ exception of 2), look just like they did before I did G2G. I still can't figure out why they didn't take when I used a decent amount of spawn when doing G2G, as to speed up the process. Some I did with a small amount of spawn also.






Bacteria. Open em up and see if they smell funny.

I would suggest agar>grain over your Liquid Contaminant.
Or test your LC on agar first.
Either way, next best step: Agar.


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Invisibleprismism
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Registered: 05/11/09
Posts: 5,570
Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
    #22050758 - 08/05/15 10:44 PM (8 years, 5 months ago)

Quote:

taGyo said:
You're new here 9mm.

Welcome to mush cult.

Do some reading :wink:




You're new here too. What's with the superiority complex? 9mm was giving out solid information and not trying to start shit like you are. All you've contributed to this thread is guff and some silly gifs.


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ephemeral anomalous


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OfflinecronicrFacebook
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism]
    #22050777 - 08/05/15 10:48 PM (8 years, 5 months ago)

And you brought? Lol...9 mm was banned and if the jars have not recovered i would say contam


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleprismism
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Registered: 05/11/09
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
    #22050795 - 08/05/15 10:51 PM (8 years, 5 months ago)

I'm just here to regulate. Also, Dink is my good friend outside of this forum.

Hope you get this issue cleared up, bud. It does sound like bacteria without any photographic help. Are you still working with agar?


--------------------
ephemeral anomalous


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OfflinecronicrFacebook
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: prismism] * 1
    #22050816 - 08/05/15 10:54 PM (8 years, 5 months ago)

Atta guy


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: G2G isn't colonizing after 5 days. Stalled? [Re: cronicr]
    #22051224 - 08/06/15 12:17 AM (8 years, 5 months ago)

Quote:

cronicr said:
your not likely to last here



Quote:

cronicr said:
.9 mm was banned



:fuckinawesome:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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