Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Kratom Capsules for Sale   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
G2G isn't colonizing after 5 days. Stalled?
    #22035408 - 08/02/15 09:25 PM (8 years, 5 months ago)

I did a G2G transfer 5-6 days ago and normally the mycelium starts showing at day 2 or 3. I even used a generous amount of colonized WBS in each jar. I used 2 different quarts of the same strand, so it shouldn't be that the spawn is bad, unless somehow how both jars of spawn were bad. Even if the spawn was bad, it should still colonize the new jar, right?

I made sure to dry the seeds on towels after 24 hr soak where they are not sticky to the touch and rattle in the jar. I'm fairly certain that all of WBS is hydrated, because they were sticking to the jar a little after PCing.

I can see a couple of seeds in some jars that have myc on them, but not fuzzy myc that shows actual expansion of mycelium like it normally would. My technique didn't change, which was using a SAB and spraying it down with soapy water and spraying an oust-like spray in the SAB to neutralize air-borne contams.

Any thoughts on the cause or solution?


--------------------


Extras: Filter Print Post Top
OfflineShroominMe
Stranger

Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22035594 - 08/02/15 10:12 PM (8 years, 5 months ago)

I don't know the cause but with a G2G you will normally have full colonization after 5 days.  Even if you don't have fae it should colonize before it stalled.

Either there was something wrong with the wbs or you got some Oust into the sub.  How long did you wait after spraying with the disinfectant?

I'd start over, sorry.


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: G2G isn't colonizing after 5 days. Stalled? [Re: ShroominMe]
    #22035610 - 08/02/15 10:15 PM (8 years, 5 months ago)

Any pics of your jars? Contams seem likely at this point.


Extras: Filter Print Post Top
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
    #22036360 - 08/03/15 04:38 AM (8 years, 5 months ago)

I can send pics but I waited the same time i normally do. 15 min before I go into the SAB.


--------------------


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22036405 - 08/03/15 05:30 AM (8 years, 5 months ago)

Quote:

I can see a couple of seeds in some jars that have myc on them, but not fuzzy myc that shows actual expansion of mycelium like it normally would.




sounds like bacteria.  post a pic.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22036636 - 08/03/15 08:28 AM (8 years, 5 months ago)

Quote:

DinkinFlicka84 said:
I made sure to dry the seeds on towels after 24 hr soak where they are not sticky to the touch and rattle in the jar. I'm fairly certain that all of WBS is hydrated, because they were sticking to the jar a little after PCing.



I think not simmering your seeds after the 24 hour soak might be the issue here. Although the seeds may have look hydrated on the outside, you want to make sure the inside of the grain is hydrated and can retain moisture. The grains will expand in size as you simmer them (15 minutes is perfect) and will look a bit puffy. Then cut the heat before you get bursted kernels which will turn into a starchy mess and clump together.


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
    #22036816 - 08/03/15 09:37 AM (8 years, 5 months ago)

Quote:

Psilosoulful said:
Quote:

DinkinFlicka84 said:
I made sure to dry the seeds on towels after 24 hr soak where they are not sticky to the touch and rattle in the jar. I'm fairly certain that all of WBS is hydrated, because they were sticking to the jar a little after PCing.



I think not simmering your seeds after the 24 hour soak might be the issue here. Although the seeds may have look hydrated on the outside, you want to make sure the inside of the grain is hydrated and can retain moisture. The grains will expand in size as you simmer them (15 minutes is perfect) and will look a bit puffy. Then cut the heat before you get bursted kernels which will turn into a starchy mess and clump together.



:asianofapproval:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
    #22036945 - 08/03/15 10:20 AM (8 years, 5 months ago)

I prepped these last night, this is after 15 min on a light simmer. Notice how the seeds are all puffed up. At this point I cut the heat, strain them (don't rinse them, you want the steam to help dry the grains), and let them sit on a window screen until the outside is mostly dry ( I use the toilet-paper test). Then load em up and you're ready to PC!! :super:



Edited by Psilosoulful (08/03/15 10:21 AM)


Extras: Filter Print Post Top
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
Re: G2G isn't colonizing after 5 days. Stalled? [Re: Psilosoulful]
    #22039059 - 08/03/15 07:03 PM (8 years, 5 months ago)

Sorry, I forgot to mention that I simmered for 10 min as well. Any other possible causes? The jar I used the G2G with was 100% colonized and looked very health. I've been doing this for about 2 years, so I'm fairly certain what looks healthy. I know that it's possible for trich to hide, but none of the jars have any mold. They're just sitting there with nothing happening. And I use 2 diff. Jars to do G2G with so as to make sure if one was bad then the other would make up for it.


--------------------


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: G2G isn't colonizing after 5 days. Stalled? [Re: DinkinFlicka84]
    #22039069 - 08/03/15 07:07 PM (8 years, 5 months ago)

bacteria dude.

grain prep method doesn't really mean much.  u can get a contam with any method.

excess moisture…kinda bad
excess incubation heat….kinda bad

both together, really bad.

thats if ur donor jar is clean.

if ur donor jar is bacterial, nothing can save it.

agar>g1 most important step if u expanding grains.  do not compromise.  spawn iffy jars instead of expanding.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Offline9mmNATO
Stranger

Registered: 08/03/15
Posts: 28
Last seen: 8 years, 5 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22039085 - 08/03/15 07:10 PM (8 years, 5 months ago)

Most likely a moisture issue, or bacterial contamn. LC culture is much better way to inoculate jars.


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22039089 - 08/03/15 07:11 PM (8 years, 5 months ago)

Quote:

9mmNATO said:
Most likely a moisture issue, or bacterial contamn. LC culture is much better way to inoculate jars.




yes, because that solves both the moisture issue, and the bacteria issue!!  genius :mindblown:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22039093 - 08/03/15 07:12 PM (8 years, 5 months ago)

:ducklol:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22039103 - 08/03/15 07:13 PM (8 years, 5 months ago)

:lmafo:


Extras: Filter Print Post Top
Offline9mmNATO
Stranger

Registered: 08/03/15
Posts: 28
Last seen: 8 years, 5 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: blindingleaf]
    #22039108 - 08/03/15 07:14 PM (8 years, 5 months ago)

I dont know if you are being smart or whatever. The common logical solution would to be start over. Lol you act like as if theres a solution to doomed fucked up jars. I threw in the LC suggestion because next time it will be even easier because of the higher volume of mycelium and multiple points of inoculation the jar will colonize quicker giving the guy some looser tolerances for fucking up the said moisture and bacterial issues.

GENIUS.


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22039119 - 08/03/15 07:16 PM (8 years, 5 months ago)

And if he's having jar problems you think he's going to pull off a clean LC?

:goodluck:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
    #22039128 - 08/03/15 07:18 PM (8 years, 5 months ago)

LC FASTER THEN G2G.....:laugh2:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offline9mmNATO
Stranger

Registered: 08/03/15
Posts: 28
Last seen: 8 years, 5 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: taGyo]
    #22039132 - 08/03/15 07:18 PM (8 years, 5 months ago)

Of course he can.


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO]
    #22039137 - 08/03/15 07:18 PM (8 years, 5 months ago)

You're new here 9mm.

Welcome to mush cult.

Do some reading :wink:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: G2G isn't colonizing after 5 days. Stalled? [Re: 9mmNATO] * 1
    #22039145 - 08/03/15 07:19 PM (8 years, 5 months ago)

IMO, an LC is another vector.

take out grain prep vector, take out possibly contaminated innoculant vector, and then I'm 100% in support of liquid cultures

i agree, they are fastest way to colonize.  but if i was having problems, that would be one of the first things i eliminate until i narrowed down what else it could be.

yea, i was being smart.  beer…u know?  take me with a grain of salt tonight, i promise, i m as laid back as they come.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: Kraken Kratom Kratom Capsules for Sale   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* HELP!!...colonization stalled jamm71 1,349 11 02/05/04 05:58 AM
by jamm71
* Re: colonization stalled Lizard King 1,612 2 06/28/00 09:11 PM
by Anonymous
* Colonization stalling??? roykinn 1,037 2 01/24/02 03:49 AM
by MAIA
* G2G transfers
( 1 2 all )
kjb1891 32,782 20 12/19/18 11:45 AM
by Nichrome
* the great sunflower seed debate.... Magician26 2,032 13 12/11/02 01:51 PM
by Skikid16
* No Colonization on Bottom Of Jars? botanist 3,376 8 03/26/03 08:30 AM
by fleshofgods
* WBS jar stalling after agar inoculation (photo) Josborne 866 14 05/04/21 02:29 PM
by Stipe-n Cap
* Top COLONIZED Bottom ISN'T KaliedoscopEyes 1,365 5 08/19/02 10:48 AM
by KaliedoscopEyes

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,899 topic views. 43 members, 328 guests and 53 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.01 seconds on 16 queries.