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OfflineNetRang3r
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Registered: 10/11/10
Posts: 35
Last seen: 8 years, 4 months
agar not growing
    #22034685 - 08/02/15 06:09 PM (8 years, 5 months ago)

so made some agar following pastywhites tek and its been 3 weeks and I see no growth on any of the agar. im trying to grow some ATL #7 and I wanted to use agar and transfer to grain but im thinking I might just have to inoc the grain with my spore syringe... any ideas what the problem might be?


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OfflineDarkhome
•Shaman•N•Training•
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Registered: 07/10/15
Posts: 517
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Re: agar not growing [Re: NetRang3r]
    #22034690 - 08/02/15 06:11 PM (8 years, 5 months ago)

Maybe I'm not understanding... Did you drop spores on the agar...or take a clone to agar....or just make agar?
Too many threads...sorry...I'm starting to go blind...lol
:sorry:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Edited by Darkhome (08/02/15 06:21 PM)


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OfflineDeTwizzle
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Re: agar not growing [Re: Darkhome]
    #22034722 - 08/02/15 06:18 PM (8 years, 5 months ago)

Sounds like he used a spore syringe as mentioned in the OP.

I had some pan cyan from a syringe never germinate on agar...nothing grew...I knocked up 3 quart jars and voila. Must have been rather old spores, especially with "exotics" that don't sell as fast on these vendors sites.

Probably your case.


Edited by DeTwizzle (08/02/15 06:22 PM)


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OfflinecronicrFacebook
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Re: agar not growing [Re: DeTwizzle]
    #22034920 - 08/02/15 07:14 PM (8 years, 5 months ago)

put some in a small half pint of grains and steal some grains once it colonizes to get to do some isolating/agar work


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineNetRang3r
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Registered: 10/11/10
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Re: agar not growing [Re: cronicr]
    #22035402 - 08/02/15 09:24 PM (8 years, 5 months ago)

thanks guys. im gonna scrap the agar and just noc some grains with the ATL (in the next couple days) I got a MS syringe im gonna test on some new agar to see if it grows.


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OfflineMachiavelliavore
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Re: agar not growing [Re: cronicr]
    #22035535 - 08/02/15 09:58 PM (8 years, 5 months ago)

Quote:

cronicr said:
put some in a small half pint of grains and steal some grains once it colonizes to get to do some isolating/agar work



:whathesaid:
No reason to prep shittons of grains when you can get all the info you need from a couple cups.

Any reason to isolate before strong fruiting strains have been identified from clone tissue?


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offline99.99
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Re: agar not growing [Re: NetRang3r]
    #22035556 - 08/02/15 10:03 PM (8 years, 5 months ago)

Quote:

NetRang3r said:
so made some agar following pastywhites tek and its been 3 weeks and I see no growth on any of the agar. im trying to grow some ATL #7 and I wanted to use agar and transfer to grain but im thinking I might just have to inoc the grain with my spore syringe... any ideas what the problem might be?




Mine worked just fine straight to grain
http://www.shroomery.org/forums/showflat.php/Number/22030275/page/1


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OfflineShroominMe
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Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: agar not growing [Re: 99.99]
    #22035645 - 08/02/15 10:27 PM (8 years, 5 months ago)

Agar is great, except when it doesn't work :rolleyes:

Go ahead and inoculate a few jars and you can continue with your agar experiments while your jars are slowly leading you to myco success :stoned:


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Offlinegoffa
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Registered: 10/20/17
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Last seen: 7 months, 27 days
Re: agar not growing [Re: ShroominMe]
    #25927096 - 04/10/19 03:10 PM (4 years, 9 months ago)

Going to have to try this...


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