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Offlinebluegill
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PF cakes to coir grow log
    #22033215 - 08/02/15 12:14 PM (8 years, 5 months ago)

This is a grow log for spawning cakes to bulk with coir. I made this thread initially (originally titled "BRF/Verm ratio and growth rates"; see original post below) because I was curious about PF tek growth rates and was wondering why there was such a difference between the batch for this project and my last batch. Both used multispore inoculant (likely the reason why), but there was a difference in substrate mixture ratios, incubation temps, light exposure, and more. Since it is MS it is really hard to say because it could be genetics as well; If I really wanted to experiment, I could have done some agar work to isolate a culture to take genetics out of the equation, but I really don't give a fuck.

Looking back at all the PF grows I have done, my non-research-backed gut feeling is that Fungifun PF tek for simple minds (http://www.fungifun.org/English/Pftek) is the best version of PF tek to follow and it seems like I get faster growth because the recipe calls for more water and a higher vermiculite to brown rice flour ratio. Of course, for all noobs to PF tek, I recommend reading the original PF guide, the one on shroomery, and watching the Let's grow mushrooms videos by RR, and make an informed decision on which guide to follow.

Some other projects I am doing with this batch include: attempting to grow in a drinking glass instead of a jar (success), cloning from a fruit to a PF jar using a syringe without a SAB (success, but I haven't ruled out contams yet). Below are pics as part of my grow log and I will document the procedures and my success/failure with spawning to bulk as well.

Grow log:

I followed the Fungifun guide. Sterilized all subs/jars on 7/28 by steaming in a stock pot.I removed foil from all the lids afterward except the drinking glass. All jars were inoculated on 7/29.

This pic was taken on 8/2, 5 days after inoculation. Seems like a speedy growth compared to the last batch. They have been incubating in a cab above a fridge at about 74-76F in the dark.


This pic was taken on 8/8, 10 days after inoculation. 5 jars are near full colonization, a couple are straggling behind. You can see my whiskey glass experiment (left foil on after sterilizing; covered holes with electrical tape) and it is growing wicked fast! I am considering stalling a few if they begin pinning before the others are ready (thanks Gyo for the tips).


Here is my clone experiment, 5 days after the tissue transfer. I see two germination points and I haven't ruled out contams yet. I also inoculated the other side, but shows no signs of growth.


I anticipate that I will be spawning these cakes by next weekend at the earliest.

**************************************************************************************************************

Original Post:

My last batch just followed the PF tek recipe, 2:1:1 ratio for verm/BRF/water. Each jar got 1cc. It was very slow growing. Incubation temps were in the 71-73 range. It took more than 3 weeks to birth all 10 cakes and some were very dehydrated at birth. My current batch follows the fungifun recipe, which specifies 3.5:1 ratio for verm/BRF and has different instructions for water. I think it was about 1-1/2 cups of water. Each jar got about 0.5cc. Incubation temps are in the 74-76 range. I am seeing explosive growth rates in 6 out of 12 jars. My whiskey glass experiment is almost halfway colonized after 4 days. I wonder if the faster growth is caused by incubation temps or the substrate mixture. Both batches are MS

On a side note, I had a couple mature fruits from the last batch that I decided to print. I took one of the stems and cut it open to poke it with a syringe full of sterilized water to try and (lol) clone it. I then took it and inoculated to a PF jar from this batch that is showing 0 signs of growth. When I stabbed the stem with the needle, I poked it multiple times, but I didn't see any visible tissue on or in the needle as I inoculated. I hope it works, but I did it purely for shits and giggles in open air. If it works, it will at least tell me whether or not the success is coming from MS. It is incubating in 71 temps too FYI


Edited by bluegill (08/29/15 09:16 PM)


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: bluegill]
    #22033414 - 08/02/15 01:05 PM (8 years, 5 months ago)

Since it's MS this really means nothing :shrug:


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22033423 - 08/02/15 01:07 PM (8 years, 5 months ago)

Those jars are also notorious for stalling.


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InvisibleFriedEggS
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22033432 - 08/02/15 01:09 PM (8 years, 5 months ago)

:whathesaid: :wow:


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Offlinebluegill
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: FriedEgg]
    #22033513 - 08/02/15 01:27 PM (8 years, 5 months ago)

Maybe it means nothing, but I sure as fuck am excited!  :cookiemonster:


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: bluegill]
    #22033541 - 08/02/15 01:32 PM (8 years, 5 months ago)

:kenthumbup:


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Offlinebluegill
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22064643 - 08/08/15 09:29 PM (8 years, 5 months ago)

Update on growth.

All jars are healthy. I'm starting to see growth in my clone experiment. (first pic)

I'm going to spawn to bulk with these. Should I just refrigerate the ones that consolidate quickest so I can spawn simultaneously? I might birth the 2 slowest ones as cakes.





--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


Edited by bluegill (08/08/15 09:31 PM)


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: bluegill]
    #22064645 - 08/08/15 09:30 PM (8 years, 5 months ago)

Just leave 'em be unless you see pins :thumbup:


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Offlinebluegill
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22064654 - 08/08/15 09:32 PM (8 years, 5 months ago)

Does it matter if they start pinning? I saw a post about "fruiting mode" and wondered if that would hurt spawning time.


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: bluegill]
    #22064660 - 08/08/15 09:34 PM (8 years, 5 months ago)

If they start pinning I'd birth them. Full colonization is a primary pinning trigger so if they've colonized 100% and digested the small bits left over and begin pinning before birthing it means the mycelium is a ready freddy.

If you're crumbling all the cakes to bulk you can use the fridge after consolidation if there appears to be a large window between the jars. Just make sure to bring it back up to room temperature over the course of a day or two, dunk for 24 hours and then crumble to bulk.

:super:


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Offlinebeatone
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22065265 - 08/09/15 12:07 AM (8 years, 5 months ago)

Uh of course incubation Temps make a difference in growth rates !!!!!!!!!


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Offlinebeatone
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22065298 - 08/09/15 12:15 AM (8 years, 5 months ago)

85 ° incubation temp, keep the humidity low. the temp for fruiting is 75° that'll also induce fruiting. Read some teks tho brother, "let's grow mushrooms" on YouTube is a good one.


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OfflinePussyFart
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: beatone]
    #22065312 - 08/09/15 12:18 AM (8 years, 5 months ago)

Quote:

beatone said:
85 ° incubation temp, keep the humidity low. the temp for fruiting is 75° that'll also induce fruiting.



Totally false info.....


Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 83F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR


Edited by PussyFart (08/09/15 12:20 AM)


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Offlinebeatone
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: PussyFart]
    #22065390 - 08/09/15 12:37 AM (8 years, 5 months ago)

Oh, will take that into consideration on my next grow. I've noticed faster colonization every time I've switched to from room temps to incubator , I currently have cakes going now. I'll message results of the alternated methods you mention. Attach sources if possible.. id like to hear more about it.


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OfflinecronicrFacebook
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: beatone]
    #22065416 - 08/09/15 12:44 AM (8 years, 5 months ago)

he is the source, that's all ya need:grin:


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: cronicr]
    #22066719 - 08/09/15 11:18 AM (8 years, 5 months ago)

Did this guy just ask a TC to attach a source?

Do some more research bro, that's standard info.

:lmafo:


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Offlinebluegill
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: beatone]
    #22066811 - 08/09/15 11:39 AM (8 years, 5 months ago)

Quote:

beatone said:
85 ° incubation temp, keep the humidity low. the temp for fruiting is 75° that'll also induce fruiting. Read some teks tho brother, "let's grow mushrooms" on YouTube is a good one.




LOL love a noob coming in here to give me advice on temps.

I do have a question though Pussyfart. What is the difference between exposing colonizing jars to a light cycle vs keeping them in the dark? I only have them in the cab to keep them at about 74F constant. Would they have more vitamin D or something?


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: bluegill]
    #22066827 - 08/09/15 11:43 AM (8 years, 5 months ago)

RogerRabbit did the experiment.

He had a whole room full of jars and noticed the ones closer to the door where light would come in colonized faster then the ones all the way in the back. Not sure the exact process or what the myc gains from the light but it seems to help.


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Offlinebluegill
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: taGyo]
    #22066847 - 08/09/15 11:47 AM (8 years, 5 months ago)

Good to know, the last grow was exposed to light and this one is not. There are too many variables to say what exactly is causing this batch to grow faster so I think I might change the thread title to something more appropriate.


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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OfflinetaGyo
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Re: BRF/Verm ratio and growth rates [log with pics!] [Re: bluegill]
    #22066886 - 08/09/15 11:55 AM (8 years, 5 months ago)

Once it's MS nothing matters :shrug:


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