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arkff7
Noob
Registered: 08/02/15
Posts: 1
Last seen: 3 years, 4 months
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No myce growth after a week
#22032874 - 08/02/15 10:53 AM (8 years, 5 months ago) |
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ive had 2 small grows before last year, but this year i cant seem to get the myce to grow. could this happen if pressure cooker fails to sterilize the brown-rice flower? (doing the cakes tek).
ive replaced the pressure cooker since i believe it wasnt sealing and simply did normal steam but have not made another run yet. figure can let these keep trying until i see contamination or something.
the only other thing i can think of is that when i heat the needle, waiting 10 seconds might not be enough to let it cool. but i dont want it to just stand there potentially gaining contams from the air.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: No myce growth after a week [Re: arkff7]
#22032946 - 08/02/15 11:08 AM (8 years, 5 months ago) |
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Carefully squirt out a tiny amount...cools the needle instantly... Don't panic if you hear a little hiss...sometimes it takes a while to germinate...give it a little longer...best to inoculate in a SAB
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
Edited by Darkhome (08/02/15 11:10 AM)
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Chakatron
JOOSA JOOSA



Registered: 07/09/13
Posts: 1,646
Loc: Your Face
Last seen: 3 months, 19 days
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Re: No myce growth after a week [Re: Darkhome]
#22033121 - 08/02/15 11:51 AM (8 years, 5 months ago) |
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Patience young grasshopper, patience.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: No myce growth after a week [Re: Chakatron]
#22033430 - 08/02/15 01:09 PM (8 years, 5 months ago) |
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Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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