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Offlinekierbob
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Registered: 06/18/15
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Last seen: 5 years, 5 months
Grain to Grain? (Pleurotus osteatus)
    #22030173 - 08/01/15 07:17 PM (8 years, 5 months ago)

Hey guys,

So I've recently found some packets of commercial spawn (Pleurotus osteatus) and I'm wondering what would be the best way to expand the spawn onto grain?

The spawn is on dried grain in a sealed foil pack.
Would I be okay to perform a grain to grain transfer straight from the foil pack without a high risk of contaminates?
Or should I play it safe and transfer some spawn to agar and clean it if needed?

All my work will be done in a SAB so I'm just worried about contaminates from the original spawn.

Thanks guys!


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OfflineHitsuzen
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Re: Grain to Grain? (Pleurotus osteatus) [Re: kierbob]
    #22030251 - 08/01/15 07:35 PM (8 years, 5 months ago)

From this thread, it might be best to isolate it on agar. That spawn might not be clean.


--------------------
Hate never yet dispelled hate,
Only love dispels hate.
This is the law,
Ancient and inexhaustible.
-Buddha


                           


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Offlinekierbob
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Re: Grain to Grain? (Pleurotus osteatus) [Re: Hitsuzen]
    #22030295 - 08/01/15 07:45 PM (8 years, 5 months ago)

Ah, don't know how I missed that thread!
It seeems I will be going the agar route for sure then.


Cheers Hitsuzen,


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OfflineHitsuzen
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Re: Grain to Grain? (Pleurotus osteatus) [Re: kierbob]
    #22030301 - 08/01/15 07:46 PM (8 years, 5 months ago)

:cheers:


--------------------
Hate never yet dispelled hate,
Only love dispels hate.
This is the law,
Ancient and inexhaustible.
-Buddha


                           


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Invisibleuncle_rico
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Re: Grain to Grain? (Pleurotus osteatus) [Re: kierbob]
    #22030327 - 08/01/15 07:52 PM (8 years, 5 months ago)

I assume the Pleurotus ostreatus is a commercial culture?  in which case, it will already be isolated.

I would take a grain and place it on fresh agar and grow it out.  This will give you a really good look at what you have and ensure it is clean.

Then, put agar wedges into grain.  Grow a clean grain jar (master).

Now,  G2G !!!!


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Offlinekierbob
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Re: Grain to Grain? (Pleurotus osteatus) [Re: uncle_rico]
    #22030373 - 08/01/15 08:00 PM (8 years, 5 months ago)

What's the ideology behind growing one master grain jar from agar and then waiting for that to colonize and then performing G2G for additional jars?

Would there be fault in making up a few agar plates and then inoculating multiple jars straight away with multiple agar wedges?

Cheers


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InvisibleMunchauzen
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Re: Grain to Grain? (Pleurotus osteatus) [Re: kierbob]
    #22030414 - 08/01/15 08:09 PM (8 years, 5 months ago)

Quote:

kierbob said:
What's the ideology behind growing one master grain jar from agar and then waiting for that to colonize and then performing G2G for additional jars?

Would there be fault in making up a few agar plates and then inoculating multiple jars straight away with multiple agar wedges?

Cheers




the term master jar just means you are expanding it. you are by no means limited to started just one jar

for instance, I have like 40 jars of rye and whole oats that I started all with little agar wedges.  I might g2g 4-5 of them, typically one of every species/variety that I am growing.

its not that a master makes for better chances or better grows... its just a quick way to produce more grain spawn once you have some going.


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Offlinekierbob
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Registered: 06/18/15
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Re: Grain to Grain? (Pleurotus osteatus) [Re: Munchauzen]
    #22030438 - 08/01/15 08:15 PM (8 years, 5 months ago)

Of course! That makes sense, always have a colonized grain jar as a master to save starting from agar again everytime.

:laugh:


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