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Amazon Shop: ½ Pint Jars, Paul Stamets

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Offlinepietruk
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Liquid inoculation techniques -Importance of stir bar?
    #2202843 - 12/26/03 10:33 PM (13 years, 6 months ago)

In Stamet's GG&MM his liguid inoc tek advises the use of a floating stir bar. these bars differ from standard stir bars in that they don't grind the organic material being stirred. Or so they say. Well my question is wheather or not it is cricial to use floating bars as they are around $60 each. This figure times 3 ( three flasks to multiply and advance the volume of mycelium) winds up being a $180 + shipping expense.

I am hoping that he is simply being a true professional / anal and this is a consideration I can play with after initial experimentation.

Also when stirring the broth for the ensuing 48 to 72 hours is any amount of heat benificial?

Thanks for any and all help.


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Offlineragadinks
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: pietruk]
    #2203456 - 12/27/03 05:45 AM (13 years, 6 months ago)

I only use normal stir bars and they work great. It works even without stir bar, but then you have to shake it sometimes to get some oxygen into the liquid.
The incubation times vary vastly depending on species, temperature, stirring or not and the composition of the liquid broth.
The longest was 20 days for a pleurotus pulmonaris without stirring at a temperature of about 20 ? Celsius ( 68 ? Fahrenheit ) using the honey tek.
The shortest was 4 days for p. pulmonaris with stirring ( normal stir bar ) at a temperature of about 24 ? Celsius ( 75,2 ? Fahrenheit ) in a liquid media that consisted of 15 g dextrose, 1 g yeast and 500 ml tap water.


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Offlinetchyted
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: pietruk]
    #2203817 - 12/27/03 12:59 PM (13 years, 6 months ago)

you are correct in your charicterization of Mr. Stamet's instructions.  you can go way lower tek than that and still achieve good results.  i don't own any of his equipment.  i use karo or honey in a standard quart mason jar, which happens to fit the cutter base of my oster blender just fine.

i never get an entire jar of mycelium, but a little goes a long way.  usually i get about a half inch of cells on the bottom of the jar.  i use a plastic lid which lets all the gas exchage needed (plus contams) :frown: 

still i get sufficient to load half a dozen syringes by just sucking the stuff in.  if it is good star-shaped or bb looking clumps, one syringe can be used as starter for half a dozen new jars, and the rest used immidiatly to innoculate whatever you want knocked.  because risk of contam increases exponentially with time i never let my licuid knock get old.


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Invisibleeric_the_red
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: pietruk]
    #2204147 - 12/27/03 04:05 PM (13 years, 6 months ago)

i put a marble in the jar to stir with.


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Offlineiggy
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: eric_the_red]
    #2204274 - 12/27/03 05:50 PM (13 years, 6 months ago)

a marble.... what a great idea. think ill try that


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Offlinepietruk
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: iggy]
    #2204283 - 12/27/03 05:58 PM (13 years, 6 months ago)

Well I'm luck in that I live near a big university which sells alot of equipment for super cheap. I've been picking up stir / hot plates for $4-7.50 Generally a bunch of other useless stuff also.

Since I have the plates I may as well speed up the inoc time.

Does anyone have any feelings as to wheather or not the type of bar has shown any significant difference?

Lastly, I imagine some heat helps things grow. I just don't want to wing up cooking my broth. Any guideline as to what temp ranges are best?

Thanks for the replies thus far.


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Invisiblemicro
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: pietruk]
    #2204525 - 12/27/03 08:46 PM (13 years, 6 months ago)

If you have a stir bar in the liquid stirring it you should be able to get it to a constant temp with a hotplate/stirrer in a couple of hours. I've never tried airating it with a stirbar, though, so I don't know what's o.k. I'll be trying it in a little bit, though -- I'll probably use one of those fat octagonal ones....

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Invisiblemycofile
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: pietruk]
    #2208241 - 12/29/03 07:08 PM (13 years, 6 months ago)

I SERIOUSLY doubt that it makes any difference what kind of stir bar you use. On the heat issue, stamets is clear in his instructions that he doesn't raise the temperature to incubation parameters, but uses room temps (75* F I think). That being said, I see no reason to raise the temp up to 84*F if you would like. I've often incubated liquid cultures at 84*F with no problems. Growth slows any hotter than this though I'm sure you knew that.

Why don't you get a bunch of those plates and offer them for trade, sell, donation? $4-7.50 is cheaper than dirt, and poor quality dirt at that.


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Offlinepietruk
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: mycofile]
    #2208264 - 12/29/03 07:18 PM (13 years, 6 months ago)

Thanks for the post,

I think some heat will be benificial. Just don't have any peramiters.

I plan to offer most of my extra stuff for trade once I get my back room tweeked out. In the meantime I pretty much pick thins up when they're supper cheap. Trading is what I'm up for most i figure middle of Jan I'll be dumping a ton.

Thanks for the reply


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Offlineragadinks
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Re: Liquid inoculation techniques -Importance of stir bar? [Re: pietruk]
    #2209239 - 12/30/03 05:37 AM (13 years, 6 months ago)

I think that Staments uses the stir bars he shows in his books because he needs to aerate a lot of liquid media ( several liters/quarts ).
Normal stir bars might be too small to stir up such a huge amount of liquid. For less amounts of liquid a smaller stir bar should be sufficient.


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Amazon Shop: ½ Pint Jars, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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