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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
My first Monotub - growlog in the making
    #22027189 - 08/01/15 07:08 AM (8 years, 5 months ago)

Going to edit this post in the next dayto make it agrow log of sorts. -3 aug

've tried the pf tek several times now, and each time the jars just develop bacteria and dark spots and half of them don't even begin to germinate (if that's the correct term). However, I tried some WBS spawned to coir/verm, and had no trouble at all.

I may make another thread as a grow log if anyone was interested in a first monotub grow, as I have pictures of most progress made. But for now, Im currently fruiting 3 quarts of spawn to about 4.5 of substrate in a 35L tub (so substrate is quite deep, and am just starting to get pins. Strain is B+.

This mushroom popped up at about 3 days past fruiting. I'm now at day 6 and have lots of other pins starting to come up, but this was aggressive as hell and grew to this size from a pin in 3 days before any others even pined (excluding sidepins)



I'm guessing that I'm going to want to be cloning this mushroom, and am probably going to clone with a LC. If successful will all mushrooms that fruit on a tub spawned with the LC all come out like this? And can a short slice of this mushroom be kept in the fridge/freezer until I'm ready to make a LC?

Sorry for the long post just for a few questions, but if anything I thought I'd just share my first mushroom with you all :smile: Can post a grow log if anyones interested, I know I enjoy reading them.


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Edited by nwalker248 (08/02/15 07:05 PM)


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Invisiblepaperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22027249 - 08/01/15 07:51 AM (8 years, 5 months ago)

I'd go to agar and skip lcs for the time being.  Pasty plates are a great way to start.  Once the culture is clean you can use it to make a grain master.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


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OfflineSyntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
Re: My first Monotub + question about cloning? [Re: paperbackwriter]
    #22027415 - 08/01/15 09:04 AM (8 years, 5 months ago)

recently I selected my clones from a tub
1. Cut at base with sterile scapel
2. Cut open the base and recieve a tissue sample
3. Transfer sample to plastic bag and store in fridge until ready to use

Ready to use?

4. Prepre agar/ pasty plates
5. Flame tweezers/ scapel
6. Transfer tissue sample to plate (preferably in a SAB)

Before you make a LC
Make some good clean culture with Pasty plates, THEN after you have cleaned culture you can make LC or transfer the plate to a grain jar, ect. The point is you can do lots of things.
Also the way genetics works is you have a higher probability of replicating those traits of a selected clone, not necessarily the exact mushroom that you have cloned


Edited by Syntheticwords (08/01/15 09:04 AM)


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: My first Monotub + question about cloning? [Re: Syntheticwords]
    #22027544 - 08/01/15 09:50 AM (8 years, 5 months ago)

Quote:

paperbackwriter said:
I'd go to agar and skip lcs for the time being.  Pasty plates are a great way to start.  Once the culture is clean you can use it to make a grain master.




Well that will probably be ready o pick within the week and i don't think i'll have enough supplies for agar that soon. so lc is probably the easiest to do so soon.
Quote:

Syntheticwords said:
recently I selected my clones from a tub
1. Cut at base with sterile scapel
2. Cut open the base and recieve a tissue sample
3. Transfer sample to plastic bag and store in fridge until ready to use

Ready to use?

4. Prepre agar/ pasty plates
5. Flame tweezers/ scapel
6. Transfer tissue sample to plate (preferably in a SAB)

Before you make a LC
Make some good clean culture with Pasty plates, THEN after you have cleaned culture you can make LC or transfer the plate to a grain jar, ect. The point is you can do lots of things.
Also the way genetics works is you have a higher probability of replicating those traits of a selected clone, not necessarily the exact mushroom that you have cloned




What do you mean by that last sentence?


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Invisiblepaperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22027578 - 08/01/15 10:03 AM (8 years, 5 months ago)

LCs should be taken to agar first to ensure a clean culture.  Even then it's dicey and agar > g2g is generally better for new cultivators.

http://www.shroomery.org/forums/showflat.php/Number/18081573/fpart/1/vc/1

If you have an asian grocery store nearby you can get agar today.  The rest of the pasty plate tek is really easy to pick up too.

And that last sentence means that whatever genetics we see expressed are within the context of the environment.  That pin for instance was right at the edge of the tub, possibly getting better FAE and humidity than the rest of your tub.

It may not pin across a whole tub like that, especially without a casing layer.

*edit* Don't get me wrong, it is a good candidate to clone.  How it fairs later on though will depend on the environment it's grown in.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


Edited by paperbackwriter (08/01/15 10:05 AM)


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: My first Monotub + question about cloning? [Re: paperbackwriter]
    #22028052 - 08/01/15 11:47 AM (8 years, 5 months ago)

Next time when doing bulk try and make your substrate a nice, smooth surface. You get better flushes that way.
Also cloning small baby pins from a cluster will be your best bet. Just pick a small pin that's growing fast in a cluster, and drop it onto agar.

Your culture will also be more aggressive on agar since it is so young, and not old if you were to take a tissue sample of a mature mushroom.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22028118 - 08/01/15 12:05 PM (8 years, 5 months ago)

I wouldn't clone a fruit that's almost at the end of its life cycle.

also you need more fae in your FC!


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: My first Monotub + question about cloning? [Re: spacechildo]
    #22028600 - 08/01/15 01:45 PM (8 years, 5 months ago)

Quote:

spacechildo said:
I wouldn't clone a fruit that's almost at the end of its life cycle.

also you need more fae in your FC!






Looks like i have a bit of reading to do on cloning and agar and things. Seems like i can save a tissue sample in the fridge for a week or so while i look into it,

Alright, I think I'm going to add a third whole each long side as the wholes are only about an inch wide. May I ask what shows that I need more fae for the future?


One more question, is it a good idea to have the fan facing directly towards the holes if i need more fae? or will this dry the substrate out? sorry


--------------------


Edited by nwalker248 (08/01/15 02:04 PM)


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Offlinespacechildo
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Registered: 01/24/13
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Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22028659 - 08/01/15 01:58 PM (8 years, 5 months ago)

your mushroom is trying to escape :wink:


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OfflinetaGyo
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Registered: 10/16/14
Posts: 18,802
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Re: My first Monotub + question about cloning? [Re: spacechildo]
    #22028885 - 08/01/15 02:50 PM (8 years, 5 months ago)

Fan facing directly will dry out the substrate. If you need more FAE, loosen the top holes.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflinecronicrFacebook
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Re: My first Monotub + question about cloning? [Re: Syntheticwords]
    #22028897 - 08/01/15 02:53 PM (8 years, 5 months ago)

Quote:

Syntheticwords said:
recently I selected my clones from a tub
1. Cut at base with sterile scapel
2. Cut open the base and recieve a tissue sample
3. Transfer sample to plastic bag and store in fridge until ready to use

Ready to use?

4. Prepre agar/ pasty plates
5. Flame tweezers/ scapel
6. Transfer tissue sample to plate (preferably in a SAB)

Before you make a LC
Make some good clean culture with Pasty plates, THEN after you have cleaned culture you can make LC or transfer the plate to a grain jar, ect. The point is you can do lots of things.
Also the way genetics works is you have a higher probability of replicating those traits of a selected clone, not necessarily the exact mushroom that you have cloned



Ya dont cut the fruit open you tear it, cutting pushes contams to the inside, i also agree with tag and space, you certainly can clone a more mature fruit bu pins are better.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: My first Monotub + question about cloning? [Re: taGyo]
    #22029210 - 08/01/15 04:04 PM (8 years, 5 months ago)

Anyone interested in a grow log when its done? hanks for all your helo, might just update here and maybe add some pictures to the op


--------------------


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: My first Monotub + question about cloning? [Re: cronicr]
    #22029216 - 08/01/15 04:05 PM (8 years, 5 months ago)

Quote:

cronicr said:
Quote:

Syntheticwords said:
recently I selected my clones from a tub
1. Cut at base with sterile scapel
2. Cut open the base and recieve a tissue sample
3. Transfer sample to plastic bag and store in fridge until ready to use

Ready to use?

4. Prepre agar/ pasty plates
5. Flame tweezers/ scapel
6. Transfer tissue sample to plate (preferably in a SAB)

Before you make a LC
Make some good clean culture with Pasty plates, THEN after you have cleaned culture you can make LC or transfer the plate to a grain jar, ect. The point is you can do lots of things.
Also the way genetics works is you have a higher probability of replicating those traits of a selected clone, not necessarily the exact mushroom that you have cloned



Ya dont cut the fruit open you tear it, cutting pushes contams to the inside, i also agree with tag and space, you certainly can clone a more mature fruit bu pins are better.




Isnt the point of cloning to get the best growing fruits so that you can repeat that? How would you know if they were just pins?


--------------------


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22029223 - 08/01/15 04:06 PM (8 years, 5 months ago)

Because you can tell.
If there's 20 pins in a cluster, and one or two are much larger and faster growing, its pretty safe to assume that will be a large mushroom.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflinecronicrFacebook
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Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22029228 - 08/01/15 04:07 PM (8 years, 5 months ago)

well it was indeed the first to push out, way more factors then size thopugh, i look for fast fruiteers and or nice clusters myself


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinespacechildo
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Re: My first Monotub + question about cloning? [Re: cronicr]
    #22029248 - 08/01/15 04:10 PM (8 years, 5 months ago)

I like those traits you cant see :smirk:

stuff like the peyote ksss is really cool tho!


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: My first Monotub + question about cloning? [Re: spacechildo]
    #22032352 - 08/02/15 08:10 AM (8 years, 5 months ago)

Damn they grow quick, this is the next picture a day apart.


How long do you think it will be until I should decapitate it? (/when spores will drop).


--------------------


Edited by nwalker248 (08/02/15 08:27 AM)


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22032449 - 08/02/15 08:54 AM (8 years, 5 months ago)

Now


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: My first Monotub + question about cloning? [Re: Buck513]
    #22033061 - 08/02/15 11:37 AM (8 years, 5 months ago)

just checked on it and the mushroom has nearly fallen over, because some sidepins have pulled the substrate away from the wall and poked up haha. might just pick it toight instead.


--------------------


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OfflinetaGyo
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Re: My first Monotub + question about cloning? [Re: nwalker248]
    #22033188 - 08/02/15 12:08 PM (8 years, 5 months ago)

It looks ready.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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