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MidwestShroomin
SlackJawwed NeckBeard



Registered: 05/21/15
Posts: 195
Loc: Orion's Belt
Last seen: 7 years, 2 months
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Spore prints without a SAB..help!
#22026758 - 08/01/15 01:01 AM (8 years, 5 months ago) |
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I want to take a spore print soon, but I have no SAB. Even if there is a contam, and I put spores on agar, I can still sector out the contaminated parts right?
I am literally going to hold a piece of foil in my hand, flat, and snip the fruit while still in the monotub and try to get it on the foil with as little air movement as possible. Then cover with a glass, place in the kitchen and wait.
Have any of you all done it like this with success? I am aware how easy it is on how to make a SAB, but for now if you can just comment on this "un-sterile" tek that would be awesome
-------------------- "A good traveller has no fixed plans and is not intent on arriving." -Lao Tzu
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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yea man use a cardboard box atleast .Its totally possible to clean up a dirty print with agar.
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MidwestShroomin
SlackJawwed NeckBeard



Registered: 05/21/15
Posts: 195
Loc: Orion's Belt
Last seen: 7 years, 2 months
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Re: Spore prints without a SAB..help! [Re: mrbart4444]
#22026809 - 08/01/15 01:20 AM (8 years, 5 months ago) |
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So just cut a piece of cardboard and use a glass to cover it and then place inside of a baggie?
Would a piece of paper bag work better?
I figure it would be easier to fold compared to cardboard..
-------------------- "A good traveller has no fixed plans and is not intent on arriving." -Lao Tzu
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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no no get ajar full of aluminum foil square slowly bring oven to 400 degress for an hour or just pc for 30 mins. then get a card board box doesnt even have to be cardboard on its side to use as the SAB then take said squares once cooled carefully sit done mushrooms cap on foil then cover with jar.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Spore prints without a SAB..help! [Re: mrbart4444]
#22026835 - 08/01/15 01:42 AM (8 years, 5 months ago) |
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foil comes off the roll sterile
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Spore prints without a SAB..help! [Re: cronicr]
#22026838 - 08/01/15 01:43 AM (8 years, 5 months ago) |
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yea not when youve owned the same roll for ten years my conscious wouldnt let me do it anyways lol.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Spore prints without a SAB..help! [Re: mrbart4444]
#22026842 - 08/01/15 01:46 AM (8 years, 5 months ago) |
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get new foil cheap ass
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MidwestShroomin
SlackJawwed NeckBeard



Registered: 05/21/15
Posts: 195
Loc: Orion's Belt
Last seen: 7 years, 2 months
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Re: Spore prints without a SAB..help! [Re: mrbart4444]
#22026854 - 08/01/15 01:50 AM (8 years, 5 months ago) |
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Thanks!running to Walmart now to ask them for some big ass boxes 
Look at em!!
-------------------- "A good traveller has no fixed plans and is not intent on arriving." -Lao Tzu
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Spore prints without a SAB..help! [Re: cronicr]
#22026859 - 08/01/15 01:54 AM (8 years, 5 months ago) |
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spare foil
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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de sure is purrty
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Spore prints without a SAB..help! [Re: mrbart4444]
#22026864 - 08/01/15 01:55 AM (8 years, 5 months ago) |
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dealer grade
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Spore prints without a SAB..help! [Re: mrbart4444]
#22026893 - 08/01/15 02:12 AM (8 years, 5 months ago) |
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Thoughts:
-You can clean it with agar
-I have had successful grows from prints taken with the method you described, though little bits of bacteria in some of them.
-Unless it came from an invitro grow, you prolly wanna throw it on agar anyway
-You can always print in a jar or a ziploc
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
Machiavelliavore said:
-You can always print in a jar or a ziploc
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MidwestShroomin
SlackJawwed NeckBeard



Registered: 05/21/15
Posts: 195
Loc: Orion's Belt
Last seen: 7 years, 2 months
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Re: Spore prints without a SAB..help! [Re: cronicr]
#22026915 - 08/01/15 02:24 AM (8 years, 5 months ago) |
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I'll try both.
Foil and a plastic bag.
Wouldn't I have to leave a little hole u the baggie for gas exchange?
-------------------- "A good traveller has no fixed plans and is not intent on arriving." -Lao Tzu
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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i would just use foil and a clean cup to cover it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Haven't used that method personally. I'd just leave it in a room without much air movement with about 1/4 of the bag unzipped. The problems with the ziplock thing is that for the open air syringe, you end up using the whole print and taking any contams left by the cap when squiriting a sterile syringe of water into the bag n shaking it up. You can be more selective off foil.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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MidwestShroomin
SlackJawwed NeckBeard



Registered: 05/21/15
Posts: 195
Loc: Orion's Belt
Last seen: 7 years, 2 months
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Thanks guys.
Wish me luck
-------------------- "A good traveller has no fixed plans and is not intent on arriving." -Lao Tzu
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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MidwestShroomin
SlackJawwed NeckBeard



Registered: 05/21/15
Posts: 195
Loc: Orion's Belt
Last seen: 7 years, 2 months
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Well that was fast lol.
Used the cardboard pieces that were cut and put them back in place:

I was only going to take 1 print. Said f it, took 2!

Once again, thanks everyone who responded!
-------------------- "A good traveller has no fixed plans and is not intent on arriving." -Lao Tzu
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