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OfflineAuoraBorealis
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Registered: 06/09/15
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Last seen: 2 years, 10 months
What's happening to my jars?
    #22026379 - 07/31/15 10:13 PM (8 years, 5 months ago)

Everything seems to be going wrong and I just cant figure out why. First, every single cake from my first attempt got green mold before the first flush was complete. NOW...when looking at my second set of jars I see yellow discoloration here and there in some of them. I also see weird mycelium growth that I am not used to. Its all still white...but pushed up against the glass i see mushroom cap shapes. Im assuming this its not them fruiting though because it all still solid white for the most part.

SO..yellow discoloration..and weird mycelium growth. I already had to get rid of 3 jars for having mold in them even though I had pressure cooked them for 2 hours prior to inoculation and had a sterile enviroment the entire time. Im really starting to get discouraged.


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Don't forget to play them some chill music. They're people too. :p


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OfflineDarkhome
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Registered: 07/10/15
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Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026386 - 07/31/15 10:15 PM (8 years, 5 months ago)

Post pics... People on here give great advice.
Yellow might just be metabolites:shrug:


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Edited by Darkhome (07/31/15 10:16 PM)


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OfflineAuoraBorealis
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Re: What's happening to my jars? [Re: Darkhome]
    #22026424 - 07/31/15 10:27 PM (8 years, 5 months ago)

i dont think posting pics will help much...never seem to be able to get decent ones. its just a light yellow discoloration...not really worried about it as much as everything else. But i definitely see a different kind of mycelium growth than my first attempt...solid white arhces...basically looks like a shroom cap was pushed against the glass. Idk if its just because its a different strain or what.


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Don't forget to play them some chill music. They're people too. :p


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OfflineSyntheticwords
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Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026502 - 07/31/15 10:59 PM (8 years, 5 months ago)



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Invisiblewildernessjunkie
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Registered: 06/13/10
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Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026505 - 07/31/15 11:00 PM (8 years, 5 months ago)

The yellow is most likely metabolites.

If you are PCing for 2 hours, I would suspect that its either your sterile process, or a less than aseptic spore sample. Probably the latter.


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OfflineLaughingcowwa
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Registered: 05/02/15
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Re: What's happening to my jars? [Re: wildernessjunkie]
    #22026559 - 07/31/15 11:16 PM (8 years, 5 months ago)

Sounds like you got a bad print there if all your cakes are giving you issues. next time you PC some jars give them a week before inoculating so you know its not a problem with your PC. Toss all the contaminations they'll only give you more problems as the contam spreads.


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Those who doubt me, suck cock by choice


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OfflineAuoraBorealis
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Re: What's happening to my jars? [Re: Laughingcowwa]
    #22026644 - 07/31/15 11:55 PM (8 years, 5 months ago)

I am so overwhelmed....there are too many methods..too many conflicting opinions. i dont see how I can possible know what I am doing wrong when I don't even know what I am doing right. I can be on these forums for days and it wont make a difference. The amount of time i had to put into this for my first attempt that was a complete failure is very discouraging cuz now I feel like I have to revamp my methods completely and try again for another 2 months. I wish there was just a single..undisputed method for mixing substrate,innoculating,incubating and fruiting. Instead I get replies that undermine everything I have been told to do up til now, including a possibility that my pressure cooker could be the problem? It seems I have no way of knowing what i did wrong which completely eliminates the possibility of trial and error and makes it more of a situation where I have to wing it and pray. I have watched countless videos and been all through these forums....there are way too many different methods and conflicting opinions for me to get anywhere.


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Don't forget to play them some chill music. They're people too. :p


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026679 - 08/01/15 12:12 AM (8 years, 5 months ago)

The best thing you can do for yourself is 1) post pics so we can all see the things in which you describe and 2) post your sterile procedure in the best detail that you can. These 2 things will help us help you.


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Offlineslartibartfast
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Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026684 - 08/01/15 12:15 AM (8 years, 5 months ago)

While it can be frustrating there are ways to figure out what is wrong. As mentioned above leaving a few jars alone after being pc'd will let you know if your pc and they way you use it are working out (No contams suggest all is fine in that department). If this works out you could PC some water and inject a small amount into a couple jars when you do  your spore syringe innoculations (if the jars inoculated with sterile water don't go bad there is a good chance that your inoculum is the problem and if some of each go bad it suggests that it is your inoculation methods that are introducing contams) It can take a while but most problems can be figured out with a little detective work and following the PF tec is about as close as you can come to a fool proof and agreed upon etc. Best of luck and don't give up, I have been doing this for years and am currently trying to figure out what is wrong with my grain spawn and while it can be time consuming to isolate and figure out what is causing the problem.
Slart


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Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit.
Trades welcome


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OfflineAuoraBorealis
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Re: What's happening to my jars? [Re: slartibartfast]
    #22026695 - 08/01/15 12:24 AM (8 years, 5 months ago)

Fair enough...I have to start somewhere... It wouldn't be as frustrating of I didn't have to move out of my apartment in 3 months. Kinda feel like I'm,on major time constraints. My pressure cooker is brand and as far as I can tell works fine. I'm just going to have to figure out what I did wrong when fruiting to have every single cake contaminated with trich before getting a flush.


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Don't forget to play them some chill music. They're people too. :p


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026739 - 08/01/15 12:48 AM (8 years, 5 months ago)

If it was your pc then you could have a bacteria problem but you mentioned that you had green mold. You don't get green mold from a pc problem because mold spores die well below pasteurization temps and I can garauntee, even if your pc wasn't working properly, it's still reaching above pasteurization temps. This means only one thing, your sterile procedure is not as up to par as it needs to be. This is where posting your sterile procedure will help us help you.

Your description of yellow discoloration most definitely can be from metabolites. What interests me and why it's crucial for you to post a pic is that we have no idea what is going on in your jars without visual aid. With the description you gave, I'm going to take a wild  guess and say your jars are pinning invitro. But without a pic, there really isn't a way for us to know for sure.

How old are your jars? Have your cakes become all white? and if so, how long has it been since they've fully colonized?


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OfflinecronicrFacebook
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Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026748 - 08/01/15 12:52 AM (8 years, 5 months ago)

if it is your syringe you will notice the mold starts right where you injected it, other then that go over every step with us from flaming and what not


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineblasteroid
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Registered: 08/01/15
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Last seen: 8 years, 4 months
Re: What's happening to my jars? [Re: AuoraBorealis]
    #22026967 - 08/01/15 03:07 AM (8 years, 5 months ago)

Hey man, I'm also just beginning and I decided to do 40 pf cakes (ha ha!) on my first attempt last year my jars also contaminated. I think the second you encounter contamination issues you should review and revise your sterilization methods and make sure you're not open air inoculating in a feces and mold filled bathroom or something like I did during my first grow.

I have encountered yellow discoloration on my most recent pf grow, 1 jar with slight yellow discoloration on the bottom and one yellow spot on the cake. I think they're metabolites or something because I birthed my jar and there were no odd smells, the yellow also never grew beyond what I spotted and never showed any signs that it was seriously damaging my cakes.

Solid white growth from what I understand is just another way the stuff grows. If your jars are going invitro though make sure they're fully colonized. I had a couple invitro jars(B+) one of which I was able to let consolidate for a week before harvest and two others which started pinning 95% colonization and then started producing fruits before I could even let them begin the consolidation phase.

Don't get discouraged. My first failed attempt I also got discouraged and didn't try again until a year later! Now I've tasted success and the fungus of my labor and I just love it. Improve your sterilizing technique. Make sure you're PCing those jars properly and everything else is on point. You'll succeed because it's just probability. From what I'm experiencing and have read properly sterilizing and properly following guidelines for proven teks nearly guarantees success.


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