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InvisibleSteveRogers
gandy dancer
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Registered: 10/24/06
Posts: 3,450
Re: Can I buy a syringe from the store to use as a spore syringe? [Re: LoveNaborFuckHater]
    #22028236 - 08/01/15 12:47 PM (8 years, 5 months ago)

If you simply make a syringe as you are saying you want to, it will more than likely be contaminated. You will end up not only with a shotgun of genetics, but also bacterial/moldy substrates more than likely.

Taking gill fragments and putting them on agar could potentially work depending on how old the specimen is and how it has been dried/stored. By the same reasoning a spore solution like you propose to make in your OP could be put onto agar.

If you successfully rehydrate and germinate spores from these dried samples, whether on agar or somehow on grains, then please update in MushCult. Others would like to see those results.

Regardless, your best option would be to obtain a fresh print or syringe somewhere online :wink: and go from there with isolates/cloning.


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"General, I am loyal to nothing......except The Dream"


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OfflineLoveNaborFuckHater
That one guy


Registered: 02/13/15
Posts: 861
Last seen: 4 years, 11 months
Re: Can I buy a syringe from the store to use as a spore syringe? [Re: SteveRogers]
    #22028530 - 08/01/15 01:30 PM (8 years, 5 months ago)

I don't plan on inncoluting with the syringe because of the risk of contamination, but rather to transfer to agar and see if I am able to get one to germinate.

I'm also considering the options of trying to force a print. I've been educating myself on mushroom hunting over the last week becuase I want to start hunting and a method I saw was if a cap has dried too much to produce spores after being picked, you can rehydrate by dropping water onto the cap or placing a sponge in the jar to raise the humidity and force a spore drop.

The gill method you said sounds promising and I might give that a try. The purpose of me trying to do this experiment though is so I don't have to order a syringe or print online. It would also be a good educational experiment


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"They told me drugs were bad, oh man, oh man, they had me fooled"


Edited by LoveNaborFuckHater (08/01/15 01:32 PM)


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InvisibleSteveRogers
gandy dancer
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Registered: 10/24/06
Posts: 3,450
Re: Can I buy a syringe from the store to use as a spore syringe? [Re: LoveNaborFuckHater]
    #22028580 - 08/01/15 01:41 PM (8 years, 5 months ago)

Good luck to you. Post results in MushCult
:fedora:


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"General, I am loyal to nothing......except The Dream"


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