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Syntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 10 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22023563 - 07/31/15 09:01 AM (8 years, 6 months ago) |
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When storing these plates it's fine to just have them sitting up right? Or flipped over?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 1 month
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Unless your plates are pooling with water I see no reason to store plates upside down
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PinPornProducer
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Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
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Hey pasty, I have 4 old colonized grain jars that have a few small area of bacteria. I cased them with coir/verm and stuck them in a mini mono. I never messed around with invitro, I just didn't want to spawn them to bulk and waste my time. Anything special I need to do with them?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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I bottom water once the pinset is in. Otherwise mist if they look dry. Thats all there really is too it.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 1 month
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22023750 - 07/31/15 09:58 AM (8 years, 6 months ago) |
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Thanks man
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burtburls
MycoAgroforestry


Registered: 09/08/14
Posts: 6
Last seen: 3 years, 2 months
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Re: Pastywhyte's Easy Agar Tek [Re: hamloaf]
#22025419 - 07/31/15 05:52 PM (8 years, 6 months ago) |
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I am also giving this a whirl, except using wide mouth half pint jars and these liquid culture lids 
Kinda just thought of the lid thing last second because I had extra, I'll report on how it works.
I used 2g LME to 2 g agar with 100 mL distilled water.
I also have some end of the boil cycle water frozen, was wondering what you thought about ratios to agar, or if you have any experience with this?
-------------------- the universe is in you theres universes in you
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: burtburls]
#22025996 - 07/31/15 08:07 PM (8 years, 6 months ago) |
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Just use the grain water as is, I usually don't bother to dilute when using it for agar.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22026005 - 07/31/15 08:10 PM (8 years, 6 months ago) |
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Quote:
Pastywhyte said: Just use the grain water as is, I usually don't bother to dilute when using it for agar.
are you using it same volume as the agar recipe?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22026010 - 07/31/15 08:11 PM (8 years, 6 months ago) |
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Quote:
Pastywhyte said: I bottom water once the pinset is in. Otherwise mist if they look dry. Thats all there really is too it.
im bottom watering from now on. with the summer heat misting has been causing some issues on dead aborts in tubs for me lately. ive recently been pulling back the liner, then running the sink in it and letting the substrate float for an hour. dump off put back into fruiting.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: eatyualive]
#22026019 - 07/31/15 08:12 PM (8 years, 6 months ago) |
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Yup all I do is a cup of grain water and 2.5 grams agar.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22026027 - 07/31/15 08:14 PM (8 years, 6 months ago) |
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need to make sure to save my next soak for the liquid cultures and the plates now.
man i have way too many pasty plates now and not enough jars to fill. ill have to crack open the bags i think. or do some a2g from hell to use up some of these plates. I feel like im back in the stone age doing A2G. its like slow motion or something.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22026031 - 07/31/15 08:15 PM (8 years, 6 months ago) |
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I have tried bottom watering bulk but it always gets water locked. To far for it to travel and too much pressure from the water already in the bulk. I find its less an issue with higher spawn ratios tho.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: eatyualive]
#22026047 - 07/31/15 08:18 PM (8 years, 6 months ago) |
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Quote:
eatyualive said: need to make sure to save my next soak for the liquid cultures and the plates now.
man i have way too many pasty plates now and not enough jars to fill. ill have to crack open the bags i think. or do some a2g from hell to use up some of these plates. I feel like im back in the stone age doing A2G. its like slow motion or something.
You will end up with a buttload of soak water once you start freezing it. I even label the type of grain it came from.
Do a tiger drop into a master and shake the hell outta it. The master will colonize very fast with that much agar and all the culture rubbing on the grains.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22026074 - 07/31/15 08:24 PM (8 years, 6 months ago) |
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Quote:
Pastywhyte said:
Quote:
eatyualive said: need to make sure to save my next soak for the liquid cultures and the plates now.
man i have way too many pasty plates now and not enough jars to fill. ill have to crack open the bags i think. or do some a2g from hell to use up some of these plates. I feel like im back in the stone age doing A2G. its like slow motion or something.
You will end up with a buttload of soak water once you start freezing it. I even label the type of grain it came from.
Do a tiger drop into a master and shake the hell outta it. The master will colonize very fast with that much agar and all the culture rubbing on the grains.
yeah i need to do that again. maybe 10 of them. Oh I got AA plus, Peu, pe, ape coming to a threatre near you. also burma clone, syzygy clone 1 and 2, pf classic clone, tc clone. the aa plus multispore syringe is clean. it went on a plate a few days ago and germiated already.
but then i have to g2g after that. i like doing 50 jars in one sitting with some LI or slurry. its like pouring people a beer only maybe a little easier. i get more for my money with the liquids rather than g2ging the quart to 10 quarts to get 6 day colonization. plus i can do it all in one sitting so it makes the clean work super easy and efficient. only so much time working 60 hours and doing the hobby for fun.
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Fly Wizard
Duder


Registered: 05/31/15
Posts: 49
Last seen: 3 years, 9 months
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Re: Pastywhyte's Easy Agar Tek [Re: eatyualive]
#22037225 - 08/03/15 11:28 AM (8 years, 6 months ago) |
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When transferring the spores over to the agar, 1) can I use a syringe a put a tiny droplet in the center of the agar? Or 2) use a sterile swab to transfer spores from the spore prints. Will both of those work?
Living in Europe I'm having trouble finding small and cheap enough plastic jars for this propose. IKEA is a possibility, so is ordering a Glad mini round pack from the UK or US, so is using petris of 100% polystyrene which is by far the cheapest.
IKEA Fotrtolig. 0.5 L
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pastywhyte's Easy Agar Tek [Re: Fly Wizard]
#22037232 - 08/03/15 11:30 AM (8 years, 6 months ago) |
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Yes both those methods will work. Might as well just let the drop fall on the center of the plate, so it has some moisture to germinate on.
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Fly Wizard
Duder


Registered: 05/31/15
Posts: 49
Last seen: 3 years, 9 months
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#22037904 - 08/03/15 02:20 PM (8 years, 6 months ago) |
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Ok. Thabks!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pastywhyte's Easy Agar Tek [Re: Fly Wizard]
#22059988 - 08/07/15 06:34 PM (8 years, 6 months ago) |
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We should have weekly/monthly sticky nominations. I nominate this sumbitch right hurr.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#22060025 - 08/07/15 06:43 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: We should have weekly/monthly sticky nominations. I nominate this sumbitch right hurr.
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 26 days
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22060556 - 08/07/15 08:54 PM (8 years, 6 months ago) |
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Second!
-------------------- "He's not altogether dense, but he's not altogether there."
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