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OfflineSillyPutty
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Registered: 07/05/13
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Rubbery Agar
    #22025957 - 07/31/15 07:56 PM (8 years, 5 months ago)

Hi,
Today I opened some agar plates that i pced in glass jar petri dish containers on June 29.

The recipe i used was...,
10 g DME
9g Agar
500Ml water

The agar must have dried out (or been cooked to long 15-10min at 15psi), because it had a very rubbery texture. The old f+ spores i dropped on them did not colonize. Upon smelling the agar it was fine, but i don't know cause this is my first agar attempt.

The agar may have been layed to thick some part were amost 2/8ths thick others around 1/16 inch.

Any advice would be appreciated.


Edited by SillyPutty (07/31/15 07:57 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Rubbery Agar [Re: SillyPutty]
    #22025976 - 07/31/15 08:02 PM (8 years, 5 months ago)

It could have dried out or its bacteria that made a film it would be hard to tell depending.


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OfflineSillyPutty
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Re: Rubbery Agar [Re: bodhisatta]
    #22025986 - 07/31/15 08:04 PM (8 years, 5 months ago)

Next time i will use myc. intstead of spores and maybe that will work.
Im concerned maybe the agar is hardening during PCing.


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InvisiblebodhisattaMDiscordReddit
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Re: Rubbery Agar [Re: SillyPutty]
    #22025990 - 07/31/15 08:05 PM (8 years, 5 months ago)

Agar hardens when it cools


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