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InvisibleMad Season
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Hidden contams * 20
    #22020260 - 07/30/15 03:05 PM (8 years, 5 months ago)

Bacteria

A prime example of hidden bacteria from kizzle:

Quote:

Kizzle said:

Sneaky bacteria


Not so sneaky bacteria



Quote:

Mad Season said:
Super sneaky.. where the hell is it kizzle lol. How do you know it has bacteria?

Is it the off center circle taking up almost all of the dish?



Quote:

Kizzle said:
It's the very light film covering the "uncolonized" area. I don't know how many dishes like that slipped by before I finally noticed it but I started to wonder why the mycelium was suddenly stalling instead of colonizing the whole plate :lol:



Quote:

blindingleaf said:
bacteria on WKZ culture.  both plates started off looking rhizo/normal, but as they aged, several sprouts popped up, and the second plate, u can see the edges of the growth are bubbling up in response to bacteria.  these are both from same clone, same day.



another one (slight bacteria/hidden something), matazapec, MS, unfruited


compare to a very clean Penis envy culture




Quote:

LocN9ne said:
Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...




Quote:

WeavieWonder said:
   




White molds


This is a random white contamination I got I wanted to share, showing how similar some white molds can be. This right here is why you NEED to be thorough. The contamination is circled.



This is another one, except the circled in part is healthy mycelium:



Since these were transfers that went wrong, I know for a fact everything outside of the spots I transfered to are contaminations (also I've been keeping a close eye on them since I noticed the white growths).

Exactly 3 weeks later

First one:

Notice how much the growth has grown out.. :thumbdown:

Second one:

The clean growth is black, but what's interesting is the mycelium colonized through the stuff highlighted in red. HOWEVER. The white growths and shit in the red shows how unorganized it is. This is something you SHOULD NEVER trust. Mycelium colonizing contams doesn't mean it vanquished it. The contamination is still there, and it's still dormant.

It's also interesting to note.. they both smelled extremely mushroomy.. If I was to use my nose, I'd think they were clean. Even though I know they're not. This is why I really don't like advising people to sniff shit (unless your spawning or birthing, then sniffing every jar is recommended :thumbup:). Your eyes are 10x better at spotting crap during colonization though.

Quote:

Kizzle said:
Here's one with hidden Aspergillus.


This is a clone of one of the agar pins.




Quote:

blindingleaf said:
here is a good pic of a hidden contam.
its verticillium fungicola and its a white mold.
it was a nuisance to me for many months.
found out it was riding on the myc, but would not show itself until a week+ after full colonization of plate.
top left is vert only
top right is vert + cube mycelium after plate consolidated for a week or two
bottom plate is healthy cube mycelium after 2 week consolidation.


note the intertwined plate has a 3d fuzz look to it.  starts in the middle, and works its way out




Quote:

bodhisatta said:


its hard to tell from pictures because of shitty white balance settings but though the cobweb on this plate looks white it was pretty off white. the cube mycelium is in the middle it's the brighter white and it was completely overcame.




cobweb is a bit of a bad word since there's cobweb disease which is a disease of the fruit body crop. and then what shroomery calls cobweb mold which can be many species of molds with a similar morphology. but may have very different spores for instance




Unknowns and mixture between the 2

Quote:

maddchef said:


From the back with a light through it



Honestly I'm not sure exactly what it is but it only showed up after about 15 days post transfer and is in every single transfer from the mother plate.

This is likely the reason I was throwing all my stuff away



This is a great example to show how sometimes it can get so intertwined you'd need to be :wizard: to get it undone.



If you look carefully, the areas that are clean are always organized. It's the main reason I go for rizmorphic organized areas


Here are some examples of organized clean plates:

For this one I would stay clear of the stuff on the bottom one at 6 o'clock.


Turned into:



Quote:

blindingleaf said:
a very clean Penis envy culture






Some species/varieties don't have rizmorphs. Like panaeolus species, but I still aspire for organization:



It's just crazy how close and hidden they all look, and makes me wonder how many contaminations I've had slip under the radar. The majority of these contams will most likely still have fruits if they don't turn green during spawning or fruiting. You'd just have a crappie pinset/BE. Or in the case of bl, a massive vert infestation on your fruits. Since I've become thorough, I RARELY see any contaminations. This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.


--------------------
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Edited by Mad Season (04/09/17 09:56 AM)


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OfflineSyntheticwords
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Re: Hidden contams [Re: Mad Season]
    #22020293 - 07/30/15 03:10 PM (8 years, 5 months ago)

I've just begun working with agar myself

I'll be keeping my eye out for these sneaky bastards

Performing my first transfer from a clone agar to another plate


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OfflinecronicrFacebook
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Re: Hidden contams [Re: Syntheticwords] * 1
    #22020298 - 07/30/15 03:12 PM (8 years, 5 months ago)

this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post


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InvisibleMad Season
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Re: Hidden contams [Re: cronicr]
    #22020330 - 07/30/15 03:17 PM (8 years, 5 months ago)

Quote:

cronicr said:
this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post




Thanks man, also I'm getting my hands on petris in bulk. You're absolutely right. That is what makes petris superior. I was actually just thinking about this the other day, and this is kind of the icing on the cake, so to speak.


--------------------
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OfflineSyntheticwords
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Re: Hidden contams [Re: Mad Season]
    #22020351 - 07/30/15 03:21 PM (8 years, 5 months ago)

I need something to not melt in the pc, to pour in my SAB


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OfflinetaGyo
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Re: Hidden contams [Re: cronicr]
    #22020365 - 07/30/15 03:24 PM (8 years, 5 months ago)

Quote:

cronicr said:
this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post



:super:


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Invisiblejbaby007
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Re: Hidden contams [Re: Mad Season]
    #22021420 - 07/30/15 06:40 PM (8 years, 5 months ago)

I've learned my lesson the hard way when it comes to hidden contams. The white molds looking so similar seemed OK to me until I ended up with a sneaky contaminated jar and g2ging it. I'm glad you guys post things like this. It really is very helpful. I'll definitely invest in some petris here soon.


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Re: Hidden contams [Re: jbaby007] * 1
    #22021624 - 07/30/15 07:18 PM (8 years, 5 months ago)

I still have a culture that has a mold intertwined so tight that you can't tell its there. Grow it out and try to fruit it and you can.

It's fucking spooky when it happens to you, you become paranoid that every piece of mycelium looks suspect.


--------------------
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InvisibleMad Season
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Re: Hidden contams [Re: maddchef]
    #22021702 - 07/30/15 07:35 PM (8 years, 5 months ago)

@synthetic, thanks :smile: and I hope it helps when you're working with agar. If you get sleeves of petris, they're pre-sterilized. They don't need to get pc'd. You can use glass petris too, but I think disposable petris are just easier, stack nicer, and nicer to look through. Plus they don't need a pc cycle.

@jbaby thanks :smile:

@madd haha honestly, if you look carefully, the areas that are clean are always organized. It's the main reason I go for rizmorphic organized areas.. However as you can see in kizzles first picture, clean and organized looking doesn't mean you're in the clear. So yeah being paranoid and thorough on agar is how you get success :thumbup:

Also do you have pics of said intertwined contamination? That'd be really cool to see :smile:


--------------------
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Edited by Mad Season (07/30/15 07:46 PM)


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OfflineKizzle
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Re: Hidden contams [Re: Mad Season]
    #22022812 - 07/31/15 01:10 AM (8 years, 5 months ago)

Here's one with hidden Aspergillus.


This is a clone of one of the agar pins.


Quote:

Plus they don't need a pc cycle.



Glass petris should really be oven sterilized anyway.


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InvisibleMad Season
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Re: Hidden contams [Re: Kizzle]
    #22023160 - 07/31/15 05:59 AM (8 years, 5 months ago)

Aspergillus! I've had a couple transfers that looked kinda fluffy/tomentose and pure white, that turned out to be aspergillus. Glad you got some pics of it kizzle :smile:


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InvisibleMad Season
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Re: Hidden contams [Re: Mad Season]
    #22045438 - 08/05/15 12:52 AM (8 years, 5 months ago)

How do I bump? :lol: god I love contams. Well it's a love hate relationship. I love it when you work your way around or through them. I'm just rambling. Cheddar cheese anyone?

In all seriousness, I hope this helps someone who's having major contamination issues realize that maybe they need to take a step back, read my improve your sterile techniques link in my signature, and try it all over again. I've had to go back to spores on agar before. Won't be the last time either


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Re: Hidden contams [Re: Mad Season]
    #22045446 - 08/05/15 12:52 AM (8 years, 5 months ago)

:bump:


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Offlinepurplecap
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Re: Hidden contams [Re: cronicr]
    #22045824 - 08/05/15 05:52 AM (8 years, 5 months ago)

nice thread. I was recently made aware that I have been probably transfering mold from one plate to the other just because I assumed that the myc of that variety was puffier. How many times can you transfer from plate to plate to keep stuff going ? I have no slants for cultures but I have a lot of petris :smile:


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InvisibleDr.Satan
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Re: Hidden contams [Re: purplecap]
    #22045895 - 08/05/15 06:33 AM (8 years, 5 months ago)

That was an interesting read :fuckinawesome:


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InvisibleMad Season
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Re: Hidden contams [Re: purplecap]
    #22046070 - 08/05/15 08:04 AM (8 years, 5 months ago)

Quote:

purplecap said:
nice thread. I was recently made aware that I have been probably transfering mold from one plate to the other just because I assumed that the myc of that variety was puffier. How many times can you transfer from plate to plate to keep stuff going ? I have no slants for cultures but I have a lot of petris :smile:



You can transfer weekly for well over 6 months. They say to g2g only 3 times max due to the amount you're expanding the mycelium, and rough housing it due to shaking it and breaking it up. It'll bring on senescence way quicker. With agar you can go well over 20 times. If you pour your dishes a bit thick, you can let it colonize and store it in the fridge in petris for a few months.

Generally I transfer once or twice to ensure it's clean. Also every variety can be fuzzy/tomentose. My ape and aa+ are extremely fuzzy looking. However there's contaminations that are fuzzy looking too :thumbdown:. That's why rizmorphic and organized is the best thing to go for. Especially when starting out.


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Invisibleblindingleaf
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Re: Hidden contams [Re: Mad Season]
    #22046133 - 08/05/15 08:22 AM (8 years, 5 months ago)

Quote:

That's why rizmorphic and organized is the best thing to go for. Especially when starting out.




QFT.
not saying tomentose can't perform, but as far as safety goes, i also go for rhizomorphic.

awesome thread man.  half of me hopes to have some more con tams to add pictures, the other half does not :P


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InvisibleMad Season
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Re: Hidden contams [Re: blindingleaf]
    #22046375 - 08/05/15 09:31 AM (8 years, 5 months ago)

:ilold: about the contams. Too true! I wanna get some more bacteria stuff. I've never really had a hidden bacteria contam problem. Not that I really should be complaining :lol:. I actually love getting contams. It's some good experience.


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InvisibleDr.Satan
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Re: Hidden contams [Re: Mad Season]
    #22046393 - 08/05/15 09:35 AM (8 years, 5 months ago)

Quote:

Mad Season said:
:ilold: about the contams. Too true! I wanna get some more bacteria stuff. I've never really had a hidden bacteria contam problem. Not that I really should be complaining :lol:. I actually love getting contams. It's some good experience.



Contams make me feel like I have poison growing in my house. :feelsbadman:


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InvisibleMad Season
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Re: Hidden contams [Re: Dr.Satan]
    #22046441 - 08/05/15 09:47 AM (8 years, 5 months ago)

:rofl: until you find out the different uses each of those contams have. Like the black mold aspergillus Niger. It can actually be eaten and used for enzymes. Penicillium is used to make penicillin and cheese. Certain yeasts can make different things with just sugar water. Like alcohol. I've heard of people isolating yeast in the air and using it to brew some insane wines.

There's lots of other things out there just waiting to be discovered through the processing of molds. Mycology is such a young science. If you get a microscope you can even start looking at the contams under the lens. Which I NEED to start doing. I can't believe I don't have a microscope yet :sad:.. here's a video showing their beauty:

But yeah for this particular hobby, contams are the bane of our existence :lol:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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InvisibleDr.Satan
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Re: Hidden contams [Re: Mad Season]
    #22046447 - 08/05/15 09:50 AM (8 years, 5 months ago)

Quote:

Mad Season said:
:rofl: until you find out the different uses each of those contams have. Like the black mold aspergillus Niger. It can actually be eaten and used for enzymes. Penicillium is used to make penicillin and cheese. Certain yeasts can make different things with just sugar water. Like alcohol. I've heard of people isolating yeast in the air and using it to brew some insane wines.

There's lots of other things out there just waiting to be discovered through the processing of molds.



That's actually some interesting stuff. I didn't know contams could be used for anything productive tbh. Learning something everyday on this site :feelsgoodman:


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InvisibleMad Season
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Re: Hidden contams [Re: Dr.Satan]
    #22046514 - 08/05/15 10:21 AM (8 years, 5 months ago)

Haha me too :smile: learning every day! Yeah molds are essential for us. There's actually lots of medicines utilizing them. Penicillin is just 1 of the most famous ones. Without penicillin I'd imagine the medical world would be a completely different place. If you're at all like me, and apparently the guys I respect on this thread :lol:, you'll start to find yourself reading up on contams, and actually liking them :O.

Also it seems to really increase your success rates if you understood where the contam came from, and how you got it. You can work around it EASILY if you know what the source is. I know I'm not the only successful grower who probably has their grow room filled with mold spores. Only place I'm really anal about is my sab


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Offlinebluegill
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Re: Hidden contams [Re: Mad Season]
    #22046526 - 08/05/15 10:25 AM (8 years, 5 months ago)

The second I get a yeast contam on agar I am going to isolate it for beer brewing.


--------------------
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InvisibleMad Season
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Re: Hidden contams [Re: bluegill]
    #22046543 - 08/05/15 10:30 AM (8 years, 5 months ago)

You need to identify it. Probably under the scope. You can't just take a random yeast and start making alcohol :lol: tho if you got a bunch of different samples, there's a chance you could accidentally come across it


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Offlinebluegill
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Re: Hidden contams [Re: Mad Season]
    #22046547 - 08/05/15 10:32 AM (8 years, 5 months ago)

Yep, two of my best friends are into beer brewing and they have wild yeast on agar right now, so they would be a lot of help! One of the yeasts is supposedly from a dog's ear.


--------------------
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InvisibleDr.Satan
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Re: Hidden contams [Re: Mad Season]
    #22046548 - 08/05/15 10:32 AM (8 years, 5 months ago)

Quote:

Mad Season said:
Haha me too :smile: learning every day! Yeah molds are essential for us. There's actually lots of medicines utilizing them. Penicillin is just 1 of the most famous ones. Without penicillin I'd imagine the medical world would be a completely different place. If you're at all like me, and apparently the guys I respect on this thread :lol:, you'll start to find yourself reading up on contams, and actually liking them :O.

Also it seems to really increase your success rates if you understood where the contam came from, and how you got it. You can work around it EASILY if you know what the source is. I know I'm not the only successful grower who probably has their grow room filled with mold spores. Only place I'm really anal about is my sab



You've convinced me to direct my reading towards contams for the day. I plained to continue reading on monos, but I feel like contams will be more interesting now :laugh:


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InvisibleMad Season
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Re: Hidden contams [Re: bluegill]
    #22046573 - 08/05/15 10:40 AM (8 years, 5 months ago)

A dog's ear... gross LOL. I'm actually trying to get into brewing too. I'm going to get some yeast on agar, and make my own malt. But that'll be way later. I got lots I want to do in mycology :wink:

@satan basically everything you need to know for monotubs: http://www.shroomery.org/forums/showflat.php/Number/20307891

I'd definitely recommend reading it if you haven't.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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InvisibleDr.Satan
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Re: Hidden contams [Re: Mad Season]
    #22046686 - 08/05/15 11:09 AM (8 years, 5 months ago)

Quote:

Mad Season said:

@satan basically everything you need to know for monotubs: http://www.shroomery.org/forums/showflat.php/Number/20307891

I'd definitely recommend reading it if you haven't.



Bookmarked that link, gunna read it tonight. Thanks man :rockon:


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Invisiblemaddchef
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Re: Hidden contams [Re: Dr.Satan]
    #22046698 - 08/05/15 11:11 AM (8 years, 5 months ago)

This plate


Is this plate from the back with a light through it



--------------------
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InvisibleMad Season
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Re: Hidden contams [Re: maddchef]
    #22046708 - 08/05/15 11:13 AM (8 years, 5 months ago)

Whoa that looks FUCKED! LOL! Is it infected with bacteria or a white mold?

Quote:

Dr.Satan said:
Quote:

Mad Season said:

@satan basically everything you need to know for monotubs: http://www.shroomery.org/forums/showflat.php/Number/20307891

I'd definitely recommend reading it if you haven't.



Bookmarked that link, gunna read it tonight. Thanks man :rockon:



Check our FrankHorrigan's dialing a monotub in like a boss if you haven't lol.


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Re: Hidden contams [Re: Mad Season]
    #22046747 - 08/05/15 11:18 AM (8 years, 5 months ago)

Yes lol.

Honestly I'm not sure exactly what it is but it only showed up after about 15 days post transfer and is in every single transfer from the mother plate.

This is likely the reason I was throwing all my stuff away


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Re: Hidden contams [Re: maddchef]
    #22046814 - 08/05/15 11:32 AM (8 years, 5 months ago)

Lol and you get your own section in the OP :lol:. If you were using plates like that, I can guarantee it was the problem lol. That's crazy looking though. :rofl: glad you shared :smile:


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Re: Hidden contams [Re: Mad Season]
    #22050619 - 08/05/15 10:15 PM (8 years, 5 months ago)

Interesting thread.  Just commenting so i can keep track.  :smile:

Petri dishes are superb.  Having a strong understanding of sterile technique is the difference between having to burn a building to the ground and a successful lab.


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Re: Hidden contams [Re: Alkaloids]
    #22051892 - 08/06/15 05:52 AM (8 years, 5 months ago)

Damn, that would explain so many of my problems ...
very informative thread !!!


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Re: Hidden contams *DELETED* [Re: Mad Season]
    #22051911 - 08/06/15 06:07 AM (8 years, 5 months ago)

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Re: Hidden contams [Re: RenegadeBlue]
    #22051968 - 08/06/15 06:41 AM (8 years, 5 months ago)

Hmm I have cloudy marshmallow looking myc in some of my jars.


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Re: Hidden contams [Re: Devon420]
    #22062296 - 08/08/15 11:24 AM (8 years, 5 months ago)

excellent idea for a thread madseason :thumbup:


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Re: Hidden contams [Re: FriedEgg]
    #22062575 - 08/08/15 12:44 PM (8 years, 5 months ago)

@renegade you'll get it down :smile: contams are also part of this hobby. When you get them you should stress where it came from. Don't go overboard and bleach the room every time. In fact I've never bleached a chamber let alone a whole room :lol:

@devon420 it's most likely normal. Mycelium behaves differently with amount of nutrients, and types of nutrients. As long as you are confident in the agar work, you really shouldn't have anything to worry about:)

@friedegg thanks man :smile: I'm surprised you found this little gem haha


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Re: Hidden contams [Re: Mad Season]
    #22062695 - 08/08/15 01:22 PM (8 years, 5 months ago)

Quote:

Mad Season said:
@friedegg thanks man :smile: I'm surprised you found this little gem haha



nothing hides from me! 

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Re: Hidden contams [Re: FriedEgg]
    #22098933 - 08/16/15 02:17 PM (8 years, 5 months ago)

bacteria on WKZ culture.  both plates started off looking rhizo/normal, but as they aged, several sprouts popped up, and the second plate, u can see the edges of the growth are bubbling up in response to bacteria.  these are both from same clone, same day.



another one (slight bacteria/hidden something), matazapec, MS, unfruited


compare to a very clean Penis envy culture


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Re: Hidden contams [Re: blindingleaf]
    #22099267 - 08/16/15 03:56 PM (8 years, 5 months ago)

:thumbup::rockon: added to op. Thanks :P


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Re: Hidden contams [Re: Mad Season]
    #22100470 - 08/16/15 09:14 PM (8 years, 5 months ago)

Quote:

This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.




i am too.  i hope we all are.  some people say they don't get con tams.

pasty said it best once, i can't remember exactly how he put it, but it was something like

"if you say you don't get contaminants, u are either lying, or ur not growing enough"

that is so true. it happens to us all.  sometimes in long streaks, sometimes short ones.  sometimes just here and there.  we are growing mushrooms indoors, in make shift clean rooms.  mold loves to hide in small crevices.  it happens to the best of us, and no one should be ashamed of it.  that kind of pride will be a persons downfall and should be avoided at all costs.  lets be real.  its ok, it doesn't make you a bad person, or a bad grower.  usually, the opposite is true, and it will make you a better one if ur observant enough about the situation.


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Re: Hidden contams [Re: blindingleaf]
    #22100491 - 08/16/15 09:21 PM (8 years, 5 months ago)

Quote:

blindingleaf said:
Quote:

This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.




i am too.  i hope we all are.  some people say they don't get con tams.

pasty said it best once, i can't remember exactly how he put it, but it was something like

"if you say you don't get contaminants, u are either lying, or ur not growing enough"

that is so true. it happens to us all.  sometimes in long streaks, sometimes short ones.  sometimes just here and there.  we are growing mushrooms indoors, in make shift clean rooms.  mold loves to hide in small crevices.  it happens to the best of us, and no one should be ashamed of it.  that kind of pride will be a persons downfall and should be avoided at all costs.  lets be real.  its ok, it doesn't make you a bad person, or a bad grower.  usually, the opposite is true, and it will make you a better one if ur observant enough about the situation.



aint that the truth well said:thumbup: seems like whenever I get a contam it's always an epic 20 jar fail lol but eh it is what it is


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Re: Hidden contams [Re: tetherface]
    #22100507 - 08/16/15 09:25 PM (8 years, 5 months ago)

lol yea, i hear that man.  truth be told, even g2g can fail us like that.
agar plates are key.  assuming technique is on point, the OG plate will always tell the whole story in those cases.


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Re: Hidden contams [Re: blindingleaf]
    #22101097 - 08/17/15 12:06 AM (8 years, 5 months ago)

Man I went for like a year and a half not able to grow a fucking thing past first flush, and only 50% of what I tried even made it that far.


But I'll be goddamned if I'm not an expert on contamination now though. Keep at it and learn as you go.


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Re: Hidden contams [Re: maddchef]
    #22122009 - 08/21/15 11:29 AM (8 years, 5 months ago)

:bump: and a quick update. This contam.. I've been keeping it around out of laziness really. Too lazy to throw it out :lol:

Quote:

This is a random white contamination I got I wanted to share, showing how similar some white molds can be. This right here is why you NEED to be thorough. The contamination is circled.



This is another one, except the circled in part is healthy mycelium:



Since these were transfers that went wrong, I know for a fact everything outside of the spots I transfered to are contaminations (also I've been keeping a close eye on them since I noticed the white growths).




Well here they are now! Exactly 3 weeks later!

First one:

Notice how much the growth has grown out.. :thumbdown:

Second one:

The clean growth is black, but what's interesting is the mycelium colonized through the stuff highlighted in red. HOWEVER. The white growths and shit in the red shows how unorganized it is. This is something you SHOULD NEVER trust. Mycelium colonizing contams doesn't mean it vanquished it. The contamination is still there, and it's still dormant.

It's also interesting to note.. they both smelled extremely mushroomy.. If I was to use my nose, I'd think they were clean. Even though I know they're not. This is why I really don't like advising people to sniff shit (unless your spawning or birthing, then sniffing every jar is recommended :thumbup:). Your eyes are 10x better at spotting crap during colonization though.


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Re: Hidden contams [Re: Mad Season]
    #22374614 - 10/13/15 05:36 PM (8 years, 3 months ago)

Quote:

Mad Season said:
If you get sleeves of petris, they're pre-sterilized.






I know this is a somewhat old thread but i was wondering if its okay to only use half of the petris in the sleeve. I know they come with some type of gas in it that keeps it sterile but once i open the sleeve is it okay to use half and then reuse them later?


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Re: Hidden contams [Re: MysticMoteToter]
    #22374968 - 10/13/15 06:36 PM (8 years, 3 months ago)

There's no sterilizing gas. They're sterilized with radiation. You just have to keep them sterile by closing off the sleeve they're before you take them out of the SAB/FH.


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Re: Hidden contams [Re: Kizzle]
    #22375480 - 10/13/15 08:12 PM (8 years, 3 months ago)

awesome man thank you!


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Re: Hidden contams [Re: MysticMoteToter]
    #23157718 - 04/26/16 03:59 PM (7 years, 9 months ago)

Anyone got more pics of hidden/meshed molds?

Ever since I suspected a few plates of having them every piece of mycelium seems suspect now.

This thread is a goldmine by the way, shame it is not full of pics. Hopefully I won't have resurrected an old thread for nothing :P.


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Re: Hidden contams [Re: Supalemonhaze]
    #23164691 - 04/28/16 10:38 AM (7 years, 8 months ago)

I wish. I haven't seen many with infused molds. Really just the one from madd is about all I've seen.


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Re: Hidden contams [Re: Mad Season]
    #23168672 - 04/29/16 09:58 AM (7 years, 8 months ago)

Too bad. Been noticing some suspect mycelium in some of my cultures. Not knowing for sure is a nuisance, guess I will have to test them out by fruiting one by one.


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Re: Hidden contams [Re: Supalemonhaze]
    #23168732 - 04/29/16 10:22 AM (7 years, 8 months ago)

Make sure they're uniform and organized. It can take many, many transfers sometimes. If they're not organized and uniform, they're not clean in my books. I've never had a culture I couldn't clean up. Even plates covered in bacteria before any cube germination I've saved with just transfers (no antibacterial agents used). I've seen lots tell others it is garbage when it's pure bacteria, but it isn't the case if you enjoy cleaning cultures lol. You get a huge sense of fulfillment when it works :smile:


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Re: Hidden contams [Re: Mad Season]
    #23171724 - 04/30/16 05:24 AM (7 years, 8 months ago)

That's the problem though, they look organised but strange, like having trouble growing over the agar surface or going from rhizo to wispy after a xfer when the recipe is the same.

I'm stuck changing my decision every 15 mins I spend staring down at them. :rofl:


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Re: Hidden contams [Re: Supalemonhaze]
    #23171743 - 04/30/16 05:44 AM (7 years, 8 months ago)

Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.



There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.



I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.



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Re: Hidden contams [Re: Supalemonhaze]
    #23171972 - 04/30/16 08:08 AM (7 years, 8 months ago)

Would you say this culture has a slight hidden bacterial issue, Mad? It was grown out with slow pin formation, and results weren't anything to write home about.



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Re: Hidden contams [Re: Supalemonhaze]
    #23172254 - 04/30/16 10:17 AM (7 years, 8 months ago)

Quote:

SloppyJoseph said:
Would you say this culture has a slight hidden bacterial issue, Mad? It was grown out with slow pin formation, and results weren't anything to write home about.





Looks off. Theres a lot of "aerial" growths. I would try to transfer until it is smooth.

Quote:

Supalemonhaze said:
Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.



There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.



I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.





So you're concerned because it grows under the surface? Like to the bottom of the dish under the agar? If you use food colouring, you'll see the myc break it down.


The first has many sectors because it still has lots of genetics in it. Keep transferring from the leading edge until you get to it looking like this:



If you're talking about the thin film around it, that is just mycelium.

The last 3 pics, the first 2 need to grow out more before making much of a call on it, but so far they look alright to me, the last one has leading edges perfect for transfers. It can take lots of transfers before they look uniform and organized.


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Re: Hidden contams [Re: Mad Season]
    #23172495 - 04/30/16 11:46 AM (7 years, 8 months ago)

Yeah, the stuff under the surface is what I meant.

I have never really looked at them with this much detail before so that's why I'm not entirely sure about it but it does seem awfully suspicious to me. I have never seen mycelium do this before. I know mycelium does colonize agar completely but never really done so like this before.

Guess I will know for sure once I try and fruit some of these.


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Re: Hidden contams [Re: Supalemonhaze]
    #23178202 - 05/01/16 10:47 PM (7 years, 8 months ago)

Quote:

Supalemonhaze said:
Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.



There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.



I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.





Sometimes that just happens, especially when transferring to a different agar mix. Keep transferring until you get a monoculture though. It's the best way to help ensure it's pure.


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Re: Hidden contams [Re: Kizzle]
    #23178328 - 05/01/16 11:17 PM (7 years, 8 months ago)

Does mycelium climbing the walls of a colonized plate definitely mean it's bacterial, or could it be because some of the agar liquid is actually on the sides of the plate (or in my case glad mini rounds)?


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Re: Hidden contams [Re: dankington]
    #23178335 - 05/01/16 11:19 PM (7 years, 8 months ago)

Myc will climb the walls regardless. Oyster myc will bust out of a plate.


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Re: Hidden contams [Re: maddchef]
    #23178362 - 05/01/16 11:29 PM (7 years, 8 months ago)

whew, thanks. That's a relief! :cool:


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Re: Hidden contams [Re: dankington]
    #23178371 - 05/01/16 11:30 PM (7 years, 8 months ago)

:awesome:


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Re: Hidden contams [Re: Mad Season]
    #23178401 - 05/01/16 11:39 PM (7 years, 8 months ago)

Mycelium normally does colonize the sides if you let it. The extent to which it seems to vary but it's not related to bacteria. You should try not let it colonize to that point though since it can grow into a contaminated part of the petri.


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Re: Hidden contams [Re: Kizzle]
    #23178687 - 05/02/16 02:31 AM (7 years, 8 months ago)

Quote:

Kizzle said:
Quote:

Supalemonhaze said:
Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.



There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.



I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.





Sometimes that just happens, especially when transferring to a different agar mix. Keep transferring until you get a monoculture though. It's the best way to help ensure it's pure.




Thanks for the re-assurance. I am still isolating it, I'll use the plates I took transfers from on grains to see what they do as well.

Pretty sure I used a shitload of spores in that inoculation, this is taking forever :lol:. God help me if I isolated and kept all the strains, I would have well over a hundred, at least. I am trying to choose around 20ish of the most good looking and rhizomorphic ones, that should give me a good isolate I reckon.


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Re: Hidden contams [Re: Mad Season]
    #24625222 - 09/13/17 03:16 AM (6 years, 4 months ago)

This is one of the most important and useful threads I've come across. Many thanks!

Marking for reference.


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New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


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Invisibleelasticaltiger
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Re: Hidden contams [Re: Mad Season]
    #25014914 - 02/22/18 11:56 PM (5 years, 11 months ago)

:trollhide:


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OfflineFailboat
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Re: Hidden contams [Re: elasticaltiger]
    #25015037 - 02/23/18 01:48 AM (5 years, 11 months ago)

I need to start doing proper transfers, just got 100 dishes so ill start in the morning. If anybodys interested in yeasts for brewing you can collect the yeasts from the air with an open container over a  few weeks the traditional way. I have some mead made this way thats got some dried cherries soaking in it meow. Also should you buy some beer you enjoy, you can collect it's yeast from the bottom of the bottles if you poured em right. Just did this the other day drom magic hats ticket to rye.


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Offlinepacmanbreed
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Re: Hidden contams [Re: Failboat]
    #25015574 - 02/23/18 09:48 AM (5 years, 11 months ago)



Mycogone hard to spot before it matures.
Damn have spend hundreds of petri. Can you still isolate once it hits?


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Invisibleplurfekt
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Re: Hidden contams [Re: pacmanbreed] * 1
    #25259028 - 06/10/18 06:56 AM (5 years, 7 months ago)

Quote:

pacmanbreed said:
Mycogone hard to spot before it matures.
Damn have spend hundreds of petri. Can you still isolate once it hits?




So I'm not the only one.. XD

Yea, it's a motherfucker.  It basically pretends to be healthy mycelium right up until pinning.  I've tossed most of my plates and am starting new ones from spore while trying to isolate healthy tissue off the most promising ones remaining.

Lost a bunch of plates, two tubs, one LC, and counting.

Also have 14 quarts that could easily be contaminated with it and there would be no way to know until fruit.

When in doubt...toss it out.  But man, that was an entire bag of Pennington Classic and 5 hours of grain prep.  Not man about the few $, but my few hours... :lol:

I'm currently reading Mad Season's thread on hidden contaminants (very helpful) and figuring out if I need a steam cleaner/flowhood or not... lol

Definitely going to revise my techniques once again and if nothing else, this is some seriously good practice/experience.

I didn't even know there was a mold that could bruise blue and hide up until fruiting.  Murphy's law...



Now, onto pouring plates and isolating healthy tissue.  Only been doing agar a few months, and this was the only contamination I've seen out of all of my plates.

Appreciate the effort put into this thread to help others.

Here's some info on mycogone I found off google:

Mycogone. This fungal disease is transmitted primarily by contaminated casing. It infects the pins or mushroom initials as they start to form but symptoms do not appear for 10-14 days. As you say - by then it is too late. The best defense is to make sure the casing is well steamed prior to application to the beds. If necessary, treatment of the casing with 1% formalin can be used if this is legal where you are growing. Treatment of the casing with benomyl, carbendazim, prochloraz manganese or thiabendazole can also be effective. If this is a chronic problem on the farm, I suggest application of these fungicides as a preventative to the casing at every crop. There has been no reported resistance to the benzimadazole fungicides but rotation of these chemicals is always a good practice if possible. Many times Mycogone is seasonal so if you have a season of the year (typically drier) when Mycogone is not so prevalent you can suspend the fungicide treatments during those months.  Sanitation and hygiene is of utmost importance and must be part of farm management year round. . If you find diseased mushrooms, weeping caps, distorted shapes, infected stipes - these mushrooms must be picked off by your best pickers wearing gloves before watering or harvest and disposed of off the farm. Watering is an important way the spores of this fungus are spread. Infected casing / compost should be isolated with heavy applications of salt or by pushing a plastic pot deep into the compost casing. Steaming after the crop has been harvested is very important to eliminate this threat from the farm. The spores can become air-borne so this must be done before cleanout.


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Offlinepacmanbreed
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Re: Hidden contams [Re: plurfekt]
    #25300228 - 06/30/18 05:13 AM (5 years, 6 months ago)

Yes mycogone can be a pain in the ass.
But it can be sometimes a false positive under a scope acording to bhod.

If my memory serves me right. I think ive grown fruits out of those plate once ive said fukit, ill try it on grains and shoeboxes.

I still wonder if i really had this contam which bothered me for months or is it something else eg. Due to Highend temp, a mutation or some bacterial response.

Hope we can identify this in the future. Will try to recreate this if i can produced fruits if i was hit by that mycogone looking plate nexttime around.

Mycogone Perniciosa AKA "wet bubble


Edited by pacmanbreed (06/30/18 05:17 AM)


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Invisibleplurfekt
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Re: Hidden contams [Re: pacmanbreed]
    #25300862 - 06/30/18 01:01 PM (5 years, 6 months ago)

Due to lack of a microscope and unnecessary debate over whether or not TC's are right in all cases, or Bod is right in -one- case, I've pretty much stopped giving a shit as I destroyed anything that could possibly contain mycogone, and bleached all my tubs/gave them a cool water bath afterwards.

Based on what I've read steam cleaning is the way to go if mycogone ever reaches fruiting stage (which if mine was mycogone, it did), as well as certain pesticides in commercial applications for farming to prevent future rashes.  The best budget steam cleaner is about 140$ on the zon' and I figured it is a solid investment for a clean freak looking to rule out vectors when I relocate.

Not a bad idea for people who live in old houses/trailers that have a really high spore load, as I've been looking at places in the country, they are common.

One clean tub does not mean that every TC has been wrong.  Also stand by not feeding that nasty shit to anyone...

Whatever, people gonna do what people gonna do

:shrug:


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OfflineFailboat
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Re: Hidden contams [Re: plurfekt]
    #25300906 - 06/30/18 01:26 PM (5 years, 6 months ago)

Steam cleaner, nice. I should get one.


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Re: Hidden contams [Re: Kizzle]
    #25306100 - 07/03/18 09:04 AM (5 years, 6 months ago)

Quote:

Kizzle said:
Here's one with hidden Aspergillus.


This is a clone of one of the agar pins.


Quote:

Plus they don't need a pc cycle.



Glass petris should really be oven sterilized anyway.



I dont see the contam in the first pic can do.eone help me out here


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Re: Hidden contams [Re: bugman]
    #25306847 - 07/03/18 05:32 PM (5 years, 6 months ago)

Its hidden in the mycelium mat it migth be on 8oclock outer edge of the dish, The mat look growth. You can see em if you look @ dishes under a light and backlit from my exp.


Edited by pacmanbreed (07/03/18 05:36 PM)


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OfflineDr3
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Re: Hidden contams [Re: pacmanbreed]
    #26513165 - 03/02/20 04:23 PM (3 years, 10 months ago)

I'm getting these white spots on 3 week old plates. Are they primordia or something like aspergillus?
Thanks




so I presume that wispy thing on the borders is mold, right?

The plate turn into this




Edited by Dr3 (03/04/20 05:14 PM)


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Offlinepolko
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Re: Hidden contams [Re: Mad Season]
    #26940038 - 09/17/20 02:19 AM (3 years, 4 months ago)

Good info! Thanks man! Beginners appreciate it!


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OfflineStevie Ray Spawn
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Re: Hidden contams [Re: Mad Season]
    #27727167 - 04/09/22 11:13 AM (1 year, 9 months ago)

Lurking newb here, trying to read as much as possible but this site is so dense with info It would take year to read it all! ha

I have some GT LC into Pc'd Millet from 3/12.  I'm ready to dump into Bod's monotub coir substrate.

I'm nt sure if my grain spawn is contaminated.  I shook up last week.

I have 7 qts, 1 pic each, any thoughts from the experts?  I have trouble telling contamination from non-contamination, 90 days into this journey.



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IN 70 days I went from Spray Kits to grain on substrate to Liquid Culture in PC'd Millet to spore swab on agar, grow tents, fans, heaters, CO2 monitors, lights, wifi controllers.  I expect to be talking to aliens by December!


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